{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:46:21Z","timestamp":1770749181809,"version":"3.50.0"},"reference-count":46,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"crossref","award":["F30AA030472"],"award-info":[{"award-number":["F30AA030472"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"crossref","award":["F30AA031632"],"award-info":[{"award-number":["F30AA031632"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"crossref","award":["R21AA029747"],"award-info":[{"award-number":["R21AA029747"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000738","name":"Veterans Affairs","doi-asserted-by":"crossref","award":["VA SRCS IK6BX004016 (BC)"],"award-info":[{"award-number":["VA SRCS IK6BX004016 (BC)"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000738","name":"Veterans Affairs","doi-asserted-by":"crossref","award":["VA Merit I01BX5845 (BC)"],"award-info":[{"award-number":["VA Merit I01BX5845 (BC)"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000027","name":"NIAAA","doi-asserted-by":"crossref","award":["T32AA007577"],"award-info":[{"award-number":["T32AA007577"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Chronic alcohol misuse is the leading cause of non-ischemic dilated cardiomyopathy, and the molecular mechanisms underlying the development of alcohol-associated cardiomyopathy (ACM), particularly regarding sex-specific susceptibility and mitochondrial contributions, are not fully known. In this study, we utilized a preclinical model of chronic + binge ethanol consumption to investigate sex differences in disease severity and mitochondrial function. Male and female C57BL\/6J mice were fed ethanol or control liquid diets for 30 days, with 2 binge episodes on days 10 and 30. Cardiac morphology was assessed via echocardiography and cardiac function via left ventricular pressure\u2013volume catheterization. Mitochondrial function was evaluated ex vivo using Seahorse XF analysis, ATP luminescence, and AmplexTM Red fluorescence in isolated ventricular mitochondria. Ethanol feeding induced significant cardiac dysfunction and increased transcriptional expression of inflammatory and fibrotic markers in males, while these effects were not seen in females. Despite these sex-specific cardiac effects, mitochondrial respiration, ATP production, collagen protein expression, and oxidative stress were not significantly altered following alcohol exposure in either sex. Further investigation is warranted to assess the potential role of ovarian hormones in this female cardioprotection against chronic + binge ethanol.<\/jats:p>","DOI":"10.3390\/cells14211682","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T11:47:19Z","timestamp":1761652039000},"page":"1682","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Female Cardioprotection in a Mouse Model of Alcohol-Associated Cardiomyopathy"],"prefix":"10.3390","volume":"14","author":[{"given":"Joshua M.","family":"Edavettal","sequence":"first","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meagan","family":"Donovan","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas R.","family":"Harris","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xavier R.","family":"Chapa-Dubocq","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keishla M.","family":"Rodr\u00edguez-Graciani","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7691-4138","authenticated-orcid":false,"given":"Janos","family":"Paloczi","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liz","family":"Simon","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8212-5354","authenticated-orcid":false,"given":"Bysani","family":"Chandrasekar","sequence":"additional","affiliation":[{"name":"Department of Medicine, School of Medicine, University of Missouri, Columbia, MO 65212, USA"},{"name":"Harry S. Truman Veterans Memorial Hospital Columbia, MO 65212, USA"},{"name":"Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65212, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason D.","family":"Gardner","sequence":"additional","affiliation":[{"name":"LSU Health Sciences Center, Department of Physiology, New Orleans, LA 70112, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2018). Global Status Report on Alcohol and Health 2018, World Health Organization. Available online: https:\/\/iris.who.int\/handle\/10665\/274603."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"853175","DOI":"10.1155\/2012\/853175","article-title":"Oxidative stress and inflammation: Essential partners in alcoholic liver disease","volume":"2012","author":"Ambade","year":"2012","journal-title":"Int. J. 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