{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T19:50:55Z","timestamp":1788292255490,"version":"build-2803163510"},"reference-count":21,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T00:00:00Z","timestamp":1762473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Through binding to complementary mRNAs, microRNAs (miRNAs) mediate gene silencing. The stability and half-life of microRNAs are controlled by two isoforms of the RNA-binding protein Roquin. This study aimed at identifying the role of Roquin to miRNA-dependent regulation of the transcriptome in the post-ischemic heart. Both Roquin isoforms are highly conserved between rats and humans and constitutively expressed in cardiomyocytes. In both cell species, hypoxia induces a down-regulation of Roquin-1 and Roquin-2. An integrative miRNA-and-mRNA analysis (MMIA) identified miR-23b-5p as a potential interaction partner of Roquins. The open data bank TargetScan8.0 suggests that the transcription factor ZBTB20 is a potential target of miR-23b-5p. The level of expression of ZBTB20 correlated with the functional recovery of rat hearts after myocardial infarction. Moreover, the down-regulation of Roquin-2 in AC16 cells by siRNA under normoxic conditions was associated with an up-regulation of miR-23b-5p and a down-regulation of ZBTB20. Furthermore, in the case of hypoxia-dependent down-regulation of Roquin, the subsequent down-regulation of ZBTB20 was reversed with the help of an antagomir against miR-23b-5p. In conclusion, hypoxia-induced down-regulation of the two Roquin isoforms was associated with an increased stability of miR-23b-5p, a Roquin-2-dependent miRNA, which subsequently led to silencing of the transcription factor ZBTB20.<\/jats:p>","DOI":"10.3390\/cells14221748","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T14:55:15Z","timestamp":1762527315000},"page":"1748","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Roquin Modulates Cardiac Post-Infarct Remodeling via microRNA Stability Control"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6457-0944","authenticated-orcid":false,"given":"Nadja","family":"Itani","sequence":"first","affiliation":[{"name":"Physiologisches Institut, Justus-Liebig-Universit\u00e4t Gie\u00dfen, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Schreckenberg","sequence":"additional","affiliation":[{"name":"Physiologisches Institut, Justus-Liebig-Universit\u00e4t Gie\u00dfen, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3017-0476","authenticated-orcid":false,"given":"Rainer","family":"Schulz","sequence":"additional","affiliation":[{"name":"Physiologisches Institut, Justus-Liebig-Universit\u00e4t Gie\u00dfen, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1936-6232","authenticated-orcid":false,"given":"Peter","family":"Bencsik","sequence":"additional","affiliation":[{"name":"Pharmahungary Group, 6722 Szeged, Hungary"},{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, 6722 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ferdinandy","sequence":"additional","affiliation":[{"name":"Pharmahungary Group, 6722 Szeged, Hungary"},{"name":"Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1094 Budapest, Hungary"},{"name":"Center for Pharmacology and Drug Research & Development, Semmelweis University, 1085 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6093-4919","authenticated-orcid":false,"given":"Klaus-Dieter","family":"Schl\u00fcter","sequence":"additional","affiliation":[{"name":"Physiologisches Institut, Justus-Liebig-Universit\u00e4t Gie\u00dfen, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,7]]},"reference":[{"key":"ref_1","first-page":"1201","article-title":"RNA-binding protein roquin negatively regulates STING-dependent innate immune response in Drosophila","volume":"42","author":"Du","year":"2020","journal-title":"Yi Chuan"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e1683","DOI":"10.1002\/wrna.1683","article-title":"The RNA regulatory programs that govern lymphocyte development and function","volume":"13","year":"2022","journal-title":"Wiley Interdiscip. 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