{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:40:34Z","timestamp":1789764034372,"version":"4.0.1"},"reference-count":65,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020423","name":"Texas Alzheimer\u2019s Research and Care Consortium","doi-asserted-by":"crossref","award":["2020-61-51-II"],"award-info":[{"award-number":["2020-61-51-II"]}],"id":[{"id":"10.13039\/100020423","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institutes of Health; Neurobiology of Aging and Alzheimer\u2019s Disease Training Grant","award":["NIH -T32 AG 020494"],"award-info":[{"award-number":["NIH -T32 AG 020494"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Alzheimer\u2019s disease (AD) is the leading cause of dementia and is often prefaced by mild cognitive impairment (MCI). Detection of AD-related changes via blood-based biomarkers would enable critical therapeutic interventions early in disease progression. Neuronal enriched extracellular vesicle (NEEV) miRNAs regulate peripheral genes as a response to early AD brain changes and hence may have biomarker potential. Plasma NEEVs were captured from plasma samples of Mexican Americans (MAs) and Non-Hispanic Whites (NHWs) using an antibody against the neuronal surface marker CD171. miRNAs isolated from NEEVs were sequenced and analyzed using miRDeep2\/DEseq2 and QIAGEN RNA-seq portal for differential expression between cognitively impaired (CI) and cognitively unimpaired controls. hsa-miR-122-5p was significantly underrepresented in the CI group in both MAs and NHWs compared to the healthy control. Other population-specific miRNAs (MAs: hsa-miR-26a-5p, hsa-let-7f-5p, and hsa-miR-139-5p, NHWs: hsa-miR-133a-3p, hsa-miR-125b-5p, and hsa-miR-100-5p) identified may have biomarker potential in AD precision medicine. Some of these differentially expressed miRNAs were associated with key AD-related comorbidities such as APOE genotype, age, and metabolic burden and were predicted to target genes within NF-\u03baB -regulated inflammatory pathways. Together, these findings suggest that dysregulated miRNA networks may serve as a mechanistic link between comorbidity burden and AD-related neuroinflammation and neurodegeneration.<\/jats:p>","DOI":"10.3390\/cells14221784","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T09:06:36Z","timestamp":1763111196000},"page":"1784","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Neuronal Enriched Extracellular Vesicle miR-122-5p as a Potential Biomarker for Alzheimer\u2019s Disease"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9429-1993","authenticated-orcid":false,"given":"Kumudu","family":"Subasinghe","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Genetics, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5962-1229","authenticated-orcid":false,"given":"Megan","family":"Rowe","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Genetics, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Population and Community Health, College of Public Health, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6857-0286","authenticated-orcid":false,"given":"Robert","family":"Barber","sequence":"additional","affiliation":[{"name":"Department of Family Medicine & Manipulative Medicine, Texas College of Osteopathic Medicine, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"},{"name":"Institute for Translational Medicine, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Genetics, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"},{"name":"Institute for Translational Medicine, UNT Health, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mahaman, Y.A.R., Embaye, K.S., Huang, F., Li, L., Zhu, F., Wang, J.Z., Liu, R., Feng, J., and Wang, X. 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