{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:17:30Z","timestamp":1780082250274,"version":"3.54.0"},"reference-count":52,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natures Science Foundation of Nantong City","award":["JC2023041"],"award-info":[{"award-number":["JC2023041"]}]},{"name":"Postgraduate Research and Practice Innovation Program of Jiangsu Province","award":["KYCX25_3838"],"award-info":[{"award-number":["KYCX25_3838"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Ginkgolic acid (GA) exhibits various biological activities, but its role in vascular restenosis remains unreported. GA (13:0) is a relatively abundant natural congener. This study aims to investigate and clarify the effects and mechanisms of GA (13:0) on vascular smooth muscle cell (VSMC) proliferation and migration in vitro, as well as on balloon injury-induced vascular restenosis in rats. The results showed that GA (13:0) significantly inhibited VSMC proliferation, migration, and intimal thickening both in vitro and in vivo. Moreover, GA (13:0) reduced the expression of cyclin D1, cyclin E1, CDK2, and CDK4, as well as cyclin D1-CDK4 and cyclin E1-CDK2 binding, leading to G0\/G1 arrest. Additionally, GA (13:0) suppressed vimentin expression and actin cytoskeleton polymerization and altered F-actin morphology. Comparative proteomics identified tectonic family member 1 (TCTN1) as a potential molecular target of GA (13:0). GA (13:0) reduced TCTN1 expression both in vitro and in vivo. Crucially, TCTN1 overexpression notably reversed the inhibitory effects of GA (13:0) on VSMC proliferation, migration, intimal thickening, expression and binding of cell cycle-related proteins, and vimentin expression. Concurrently, TCTN1 overexpression also reversed GA (13:0)-induced F-actin depolymerization and rearrangement and G0\/G1 arrest. GA (13:0) significantly inhibited TCTN1 co-localization with vimentin and actin in vitro and in vivo. Furthermore, we found that CCCTC binding factor (CTCF) binds to the 162\u2013176 site of the TCTN1 promoter to regulate TCTN1 transcription, and CTCF knockout significantly down-regulated TCTN1 protein levels. This study reveals that GA (13:0) inhibits TCTN1 transcription and expression, hindering G1\/S transition, vimentin expression, and F-actin rearrangement, thereby suppressing vascular restenosis.<\/jats:p>","DOI":"10.3390\/cells14231922","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T15:02:48Z","timestamp":1764774168000},"page":"1922","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ginkgolic Acid Inhibits VSMC Proliferation and Migration and Vascular Restenosis by Regulating Cell Cycle Progression and Cytoskeleton Rearrangement Through TCTN1"],"prefix":"10.3390","volume":"14","author":[{"given":"Yuting","family":"Shao","sequence":"first","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyan","family":"Yi","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Nantong University, 9 Seyuan Road, Nantong 226019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jacc.2015.05.002","article-title":"Late restenosis after paclitaxel-coated balloon angioplasty occurs in patients with drug-eluting stent restenosis","volume":"66","author":"Habara","year":"2015","journal-title":"J. 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