{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T07:22:31Z","timestamp":1772349751156,"version":"3.50.1"},"reference-count":81,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel, and its activator, the alcohol breakdown product acetaldehyde, plays a key role in the pathomechanism of alcoholic liver disease (ALD). We hypothesized that TRPA1 is expressed in the liver, can be activated by alcohol breakdown products, and plays a role in ALD. We aimed (1) to confirm the presence of TRPA1 in liver samples from C57BL6\/J mice by RNAscope in situ hybridization combined with immunostaining, (2) to prove that alcohol breakdown products may activate human TRPA1 by calcium-imaging, and (3) to investigate the role of TRPA1 in a chronic continuous 20% alcohol drinking model involving Trpa1 gene-deficient (KO) mice. The liver enzyme levels were evaluated; moreover, the steatosis, portal and interface inflammatory infiltrations were assessed in PAS\u2013hematoxylin-stained sections. We detected Trpa1 expression in both hepatocytes and liver macrophages. We observed elevated liver enzyme levels in wild-type mice. Significant inflammatory infiltration and steatosis developed in both WT and KO mice in response to alcohol; however, no significant differences were found between the genotypes. We conclude that Trpa1 is expressed in hepatocytes and liver macrophages; however, the chronic alcohol-induced steatosis and inflammatory infiltration develop through a TRPA1-independent mechanism.<\/jats:p>","DOI":"10.3390\/cells15050423","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:05:16Z","timestamp":1772211916000},"page":"423","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TRPA1 Expressed by Hepatocytes and Liver Macrophages Does Not Mediate Inflammatory Infiltration and Steatosis in a Mouse Model of Chronic Alcohol-Induced Liver Injury"],"prefix":"10.3390","volume":"15","author":[{"given":"Dorottya Luca","family":"Feh\u00e9r","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4175-9402","authenticated-orcid":false,"given":"Ammar","family":"Al-Omari","sequence":"additional","affiliation":[{"name":"Department of Cosmetic Science, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, Jordan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zolt\u00e1n","family":"S\u00e1ndor","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e1niel","family":"Heged\u00fcs","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2830-2732","authenticated-orcid":false,"given":"Bal\u00e1zs","family":"Gaszner","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Szombati","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e1s","family":"Fincsur","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3360-2425","authenticated-orcid":false,"given":"Vikt\u00f3ria","family":"Kormos","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, Medical School, University of P\u00e9cs, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2026, February 24). 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