{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:17:41Z","timestamp":1790201861885,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Novel therapeutic strategies are required to protect the heart from acute ischaemia-reperfusion injury (IRI) and improve outcomes in patients with acute myocardial infarction (AMI). Mitochondria play a critical role in determining cardiomyocyte fate following acute IRI, with genetic and pharmacological inhibition of Drp1-mediated mitochondrial fission limiting cardiomyocyte death. We investigated the role of the mitochondrial Drp1 receptors, MiD49 and MiD51, as novel targets for cardioprotection. In cardiac cell lines subjected to simulated IRI, dual genetic knockdown of both MiD49 and MiD51 reduced cell death, inhibited mitochondrial fission, prevented mitochondrial permeability transition pore opening, and attenuated mitochondrial calcium overload compared with wild-type cells. However, individual knockdown of either MiD49 or MiD51 did not induce mitochondrial elongation or inhibit MPTP opening. Whole-body genetic ablation of MiD49 in adult mice modestly altered mitochondrial morphology but did not affect myocardial infarct size or cardiac function following AMI. Together with the in vitro protection seen with dual MiD49\/51 knockdown, these findings suggest that MiD49 deficiency alone is insufficient and that coordinated inhibition of MiD49 and MiD51 may be required for cardioprotection.<\/jats:p>","DOI":"10.3390\/cells15060559","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T11:44:46Z","timestamp":1774007086000},"page":"559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mitochondrial Dynamic Proteins MiD49 and MiD51 as Novel Targets of Cardioprotection"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2127-1869","authenticated-orcid":false,"given":"Parisa","family":"Samangouei","sequence":"first","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"},{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"},{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2283-6434","authenticated-orcid":false,"given":"Gustavo E.","family":"Crespo-Avilan","sequence":"additional","affiliation":[{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"},{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Hall","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sauri","family":"Hernandez-Resendiz","sequence":"additional","affiliation":[{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"},{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Maeve","family":"Elder","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura D.","family":"Osellame","sequence":"additional","affiliation":[{"name":"La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3083, Australia"},{"name":"Olivia Newton-John Cancer Research Institute, Heidelberg, VIC 3084, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole G. Z.","family":"Tee","sequence":"additional","affiliation":[{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khairunnisa","family":"Katwadi","sequence":"additional","affiliation":[{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Bing","family":"Ong","sequence":"additional","affiliation":[{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1278-7408","authenticated-orcid":false,"given":"Xiu-Yi","family":"Kwek","sequence":"additional","affiliation":[{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siavash","family":"Beikoghli Kalkhoran","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"},{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"},{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niall","family":"Burke","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7791-9320","authenticated-orcid":false,"given":"Derek M.","family":"Yellon","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek J.","family":"Hausenloy","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, Institute of Cardiovascular Science, University College London, London WC1E 6HX, UK"},{"name":"National Heart Research Institute Singapore, National Heart Centre, Singapore 16909, Singapore"},{"name":"Cardiovascular and Metabolic Disorders Program, Duke-National University of Singapore Medical School, Singapore 169857, Singapore"},{"name":"Yong Loo Lin School of Medicine, National University Singapore, Singapore 117597, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e139","DOI":"10.1161\/CIR.0000000000000757","article-title":"Heart Disease and Stroke Statistics\u20142020 Update: A Report from the American Heart Association","volume":"141","author":"Virani","year":"2020","journal-title":"Circulation"},{"key":"ref_2","first-page":"1082","article-title":"2017 ESC Guidelines for the management of acute myocardial infarction in patients presenting with ST-segment elevation","volume":"70","author":"Ibanez","year":"2017","journal-title":"Rev. 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