{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:53:05Z","timestamp":1785433985069,"version":"3.56.0"},"reference-count":24,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["22K19390"],"award-info":[{"award-number":["22K19390"]}]},{"DOI":"10.13039\/501100004043","name":"Kanazawa Medical University","doi-asserted-by":"publisher","award":["K2025-3"],"award-info":[{"award-number":["K2025-3"]}],"id":[{"id":"10.13039\/501100004043","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004043","name":"Kanazawa Medical University","doi-asserted-by":"publisher","award":["K2025-16"],"award-info":[{"award-number":["K2025-16"]}],"id":[{"id":"10.13039\/501100004043","id-type":"DOI","asserted-by":"publisher"}]},{"award":["K2025-3"],"award-info":[{"award-number":["K2025-3"]}],"id":[{"id":"https:\/\/ror.org\/0535cbe18","id-type":"ROR","asserted-by":"publisher"}]},{"award":["K2025-16"],"award-info":[{"award-number":["K2025-16"]}],"id":[{"id":"https:\/\/ror.org\/0535cbe18","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The C-terminus of the Y14 protein, which is also known as RBM8A and is encoded by the gene responsible for human thrombocytopenia absent radius syndrome, contains two serines that undergo phosphorylation inside an intrinsically disordered region (IDR). Although both serines are frequently phosphorylated in cells, their biological role remains unclear; therefore, we estimated the peptide structure using PEPstrMOD, which predicts peptide conformations through molecular dynamics. For this analysis, amino acid residues 151\u2013174 of Y14, identified as an IDR in UniProt, were targeted. Structural prediction via PEPstrMOD revealed that the target peptide adopts an elongated structure in its unphosphorylated state, while simulating its phosphorylated state revealed an increase in hydrogen bonds and a more compact conformation. The compact structure of Y14 induced by phosphorylation may aid in the formation of the exon\u2013exon junction complex at the exon\u2013exon junction, which facilitates mRNA transport and translation. The prevalence of phosphorylated Y14 in cells may indicate that this higher-order structure is also essential for mRNA metabolism.<\/jats:p>","DOI":"10.3390\/cells15070648","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T04:10:27Z","timestamp":1775448627000},"page":"648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Estimation of Double-Serine Phosphorylation\u2019s Effects on the Intrinsically Disordered Region Structure in Y14 (RBM8A) Protein via Molecular Dynamics Simulation"],"prefix":"10.3390","volume":"15","author":[{"given":"Yuka","family":"Nakamura","sequence":"first","affiliation":[{"name":"Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2095-9425","authenticated-orcid":false,"given":"Tetsuhiro","family":"Horie","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-4510","authenticated-orcid":false,"given":"Takuya","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8731-3015","authenticated-orcid":false,"given":"Hisayoshi","family":"Yoshizaki","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, School of Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-1070","authenticated-orcid":false,"given":"Hideaki","family":"Okajima","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, School of Medicine, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1012-8430","authenticated-orcid":false,"given":"Yasuhito","family":"Ishigaki","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku 920-0293, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1261\/rna.080334.124","article-title":"Exploring the therapeutic potential of modulating nonsense-mediated mRNA decay","volume":"31","author":"McMahon","year":"2025","journal-title":"RNA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"155677","DOI":"10.1016\/j.prp.2024.155677","article-title":"Nonsense-mediated mRNA decay: Physiological significance, mechanistic insights and future implications","volume":"264","author":"Patro","year":"2024","journal-title":"Pathol. 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