{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:59:08Z","timestamp":1785437948764,"version":"3.56.0"},"reference-count":65,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CAM, Consejeria de Educaci\u00f3n Ciencia y Universidades","award":["IND2024\/bio-34751"],"award-info":[{"award-number":["IND2024\/bio-34751"]}]},{"award":["IND2024\/bio-34751"],"award-info":[{"award-number":["IND2024\/bio-34751"]}],"id":[{"id":"https:\/\/ror.org\/038tahs86","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Biology Department at Universidad Autonoma de Madrid","award":["BIOUAM07-2021"],"award-info":[{"award-number":["BIOUAM07-2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Non-invasive assessment of oocyte quality remains a challenge in assisted reproductive technology (ART). Through their bidirectional communication with the gamete, cumulus cells (CCs) act as a functional mirror of oocyte competence; however, the specific angiogenic signature within this microenvironment is still poorly understood. In the present study, we performed RNA-seq on CCs from healthy oocyte donors and infertile patients, utilizing a multi-pipeline bioinformatic approach (STAR-Cufflinks, TopHat-HTSeq, and HISAT2-StringTie) to establish a high-confidence, exploratory transcriptomic profile. A set of 234 differentially expressed genes (DEGs) consistently identified across pipelines was obtained, with functional enrichment highlighting blood vessel morphogenesis and angiogenesis as primary drivers of transcriptomic divergence between groups. RT-qPCR validation in individual samples confirmed statistically significant differences for ANKRD22 (upregulated) and E2F7 (downregulated) in infertile patients, while other angiogenesis-related genes, including ANGPT1, ANGPT2 and THBS1, showed consistent but non-significant expression trends, suggesting alterations in angiogenesis-related processes within the follicular microenvironment. These findings support the presence of coordinated angiogenesis-related alterations in cumulus cells and provide a basis for future studies exploring their potential relevance in oocyte competence and ART outcomes.<\/jats:p>","DOI":"10.3390\/cells15080677","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:38:26Z","timestamp":1776080306000},"page":"677","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNA-Seq Analysis of Human Cumulus Cells Identifies Angiogenic Pathways Associated with Infertility"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5937-6514","authenticated-orcid":false,"given":"Alejandro","family":"Baratas","sequence":"first","affiliation":[{"name":"Grupo REDA, Departamento de Biolog\u00eda (Gen\u00e9tica), Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"P\u00e9rez-Quiroga","sequence":"additional","affiliation":[{"name":"Grupo REDA, Departamento de Biolog\u00eda (Gen\u00e9tica), Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3730-9549","authenticated-orcid":false,"given":"Rosario","family":"Planello","sequence":"additional","affiliation":[{"name":"Grupo de Entomolog\u00eda Molecular, Biomarcadores y Estr\u00e9s Ambiental, Facultad de Ciencias, Universidad Nacional de Educaci\u00f3n a Distancia (UNED), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2817-8997","authenticated-orcid":false,"given":"M\u00f3nica","family":"Aquilino","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Gen\u00e9tica, Facultad Ciencias de la Salud, Universidad Rey Juan Carlos, 28922 Alcorc\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1621-3102","authenticated-orcid":false,"given":"Magdalena","family":"Serrano","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA-CSIC), Ctra. de La Coru\u00f1a km 7,5, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mois\u00e9s de la","family":"Casa","sequence":"additional","affiliation":[{"name":"GINEFIV, Assisted Reproduction Centre, 28703 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosu","family":"Franco-Iriarte","sequence":"additional","affiliation":[{"name":"Hospital Ruber Internacional, 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0729-3849","authenticated-orcid":false,"given":"Rosa","family":"Roy","sequence":"additional","affiliation":[{"name":"Grupo REDA, Departamento de Biolog\u00eda (Gen\u00e9tica), Facultad de Ciencias, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103614","DOI":"10.1016\/j.semcdb.2025.103614","article-title":"The role of granulosa cells in oocyte development and aging: Mechanisms and therapeutic opportunities","volume":"171","author":"Wang","year":"2025","journal-title":"Semin. 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