{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:45:32Z","timestamp":1785415532046,"version":"3.56.0"},"reference-count":20,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Anusandhan National Research Foundation","award":["CRG\/2020\/002100"],"award-info":[{"award-number":["CRG\/2020\/002100"]}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"crossref","award":["79\/I\/IIRP-NIOP\/Onco\/2023-NCD-III"],"award-info":[{"award-number":["79\/I\/IIRP-NIOP\/Onco\/2023-NCD-III"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]},{"award":["79\/I\/IIRP-NIOP\/Onco\/2023-NCD-III"],"award-info":[{"award-number":["79\/I\/IIRP-NIOP\/Onco\/2023-NCD-III"]}],"id":[{"id":"https:\/\/ror.org\/0492wrx28","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Gallbladder cancer (GBC) is an aggressive malignancy with limited treatment options and poor clinical outcomes. Identifying novel molecular targets is critical for improving therapeutic strategies. Sorcin (SRI), a calcium-binding protein implicated in tumor progression, has not been comprehensively investigated in GBC. SRI expression was analyzed by immunohistochemistry (IHC) in a large cohort of gallstone disease (GSD) controls (n = 85) and GBC tissues (n = 85). Functional assays, including cell proliferation, wound healing, transwell invasion, and Western blot analyses of epithelial\u2013mesenchymal transition (EMT) markers, were performed in the NOZ GBC cell line following siRNA-mediated SRI knockdown. IHC revealed that 67% of GBC cases exhibited positive staining whereas all the GSD cases exhibited negative staining of SRI, demonstrating a significant upregulation of SRI in GBC (p &lt; 0.001). SRI knockdown resulted in reduced proliferative capacity and markedly impaired migration and invasion. Further, SRI knockdown decreased vimentin levels, indicating suppression of EMT. SRI is significantly overexpressed in GBC and promotes key oncogenic traits, including proliferation, migration, invasion, and EMT. These findings highlight SRI as a potential therapeutic target in GBC. Further validation in animal models may facilitate translation into clinical applications.<\/jats:p>","DOI":"10.3390\/cells15080678","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:38:26Z","timestamp":1776080306000},"page":"678","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein Expression Analysis and Functional Characterization of Sorcin in Gallbladder Cancer"],"prefix":"10.3390","volume":"15","author":[{"given":"Vaishali","family":"Jain","sequence":"first","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"},{"name":"Faculty of Health Sciences, Manipal Academy of Higher Education (MAHE), Manipal 576104, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Saklani","sequence":"additional","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"},{"name":"Faculty of Health Sciences, Manipal Academy of Higher Education (MAHE), Manipal 576104, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srishti","family":"Kawatra","sequence":"additional","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"},{"name":"Faculty of Health Sciences, Manipal Academy of Higher Education (MAHE), Manipal 576104, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7286-5941","authenticated-orcid":false,"given":"Puja","family":"Sakhuja","sequence":"additional","affiliation":[{"name":"Department of Pathology, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi 110002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surbhi","family":"Goyal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi 110002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Department of Surgery, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research (GIPMER), New Delhi 110002, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6857-2425","authenticated-orcid":false,"given":"Parveen","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7643-7936","authenticated-orcid":false,"given":"Fouzia","family":"Siraj","sequence":"additional","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"},{"name":"Faculty of Health Sciences, Manipal Academy of Higher Education (MAHE), Manipal 576104, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3804-6157","authenticated-orcid":false,"given":"Poonam","family":"Gautam","sequence":"additional","affiliation":[{"name":"ICMR-Centre for Cancer Pathology (Formerly a Part of ICMR-National Institute of Pathology), Safdarjung Hospital Campus, New Delhi 110029, India"},{"name":"Faculty of Health Sciences, Manipal Academy of Higher Education (MAHE), Manipal 576104, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,12]]},"reference":[{"key":"ref_1","first-page":"22","article-title":"Gallbladder cancer","volume":"8","author":"Roa","year":"2022","journal-title":"Nat. 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