{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:08:15Z","timestamp":1785434895930,"version":"3.56.0"},"reference-count":55,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Institute of Health (NIH)\/ National Eye Institute","doi-asserted-by":"publisher","award":["R01EY032495"],"award-info":[{"award-number":["R01EY032495"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"award":["R01EY032495"],"award-info":[{"award-number":["R01EY032495"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018719","name":"University of Missouri School of Medicine","doi-asserted-by":"publisher","award":["Research start-up funds"],"award-info":[{"award-number":["Research start-up funds"]}],"id":[{"id":"10.13039\/100018719","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Research start-up funds"],"award-info":[{"award-number":["Research start-up funds"]}],"id":[{"id":"https:\/\/ror.org\/02ets8c94","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Zika virus (ZIKV) remains a significant global public health threat due to its association with severe neurological and ocular abnormalities, including microcephaly and congenital glaucoma in infants. Viruses often exploit host metabolic programs, such as energy and lipid metabolism, to support their replication. However, how ZIKV-driven metabolic reprogramming affects the anterior segment of the eye, especially trabecular meshwork (TM) cells, remains poorly defined. In this study, we investigated the roles of AMP-activated protein kinase (AMPK) signaling, fatty acid (FA) metabolism, and lipid droplet (LD) biogenesis in ZIKV-induced ocular pathogenesis using primary human TM cells and an IFNAR1-deficient mouse model. ZIKV infection triggered time-dependent activation of the LKB1-AMPK-ACC signaling axis and significantly increased LD accumulation. Pharmacological activation of AMPK suppressed viral replication, whereas its inhibition enhanced infection, highlighting an antiviral role for AMPK signaling. In contrast, ZIKV promoted LD biogenesis, and inhibition of DGAT1 reduced both LD formation and viral replication, indicating a proviral role for LDs. Modulation of FA metabolism further revealed differential effects on ZIKV infection: saturated FA (palmitate) enhanced viral replication, whereas inhibition of FA oxidation with etomoxir reduced infection. Conversely, unsaturated FAs (oleate and linoleate) suppressed viral replication, in part by impairing viral binding and entry. Collectively, these findings show that ZIKV reshapes host metabolic pathways in TM by differentially engaging AMPK signaling, FA metabolism, and LD biogenesis to promote viral replication and spread in ocular tissue. Targeting these metabolic pathways may offer promising therapeutic avenues for preventing and\/or treating ZIKV-associated ocular complications.<\/jats:p>","DOI":"10.3390\/cells15090817","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T10:47:28Z","timestamp":1777546048000},"page":"817","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zika Virus-Induced Metabolic Reprogramming Drives Lipid Droplet Biogenesis, Promoting Viral Replication and Ocular Pathogenesis"],"prefix":"10.3390","volume":"15","author":[{"given":"Prince","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Mason Eye Institute, University of Missouri School of Medicine, 1 Hospital Dr, Columbia, MO 65212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Mason Eye Institute, University of Missouri School of Medicine, 1 Hospital Dr, Columbia, MO 65212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Mason Eye Institute, University of Missouri School of Medicine, 1 Hospital Dr, Columbia, MO 65212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5517-1120","authenticated-orcid":false,"given":"Pawan Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Mason Eye Institute, University of Missouri School of Medicine, 1 Hospital Dr, Columbia, MO 65212, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101209","DOI":"10.1016\/j.spen.2025.101209","article-title":"Pediatric Zika virus: The virus and its neurologic impact","volume":"54","author":"Rojas","year":"2025","journal-title":"Semin. Pediatr. Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mittal, R., Nguyen, D., Debs, L.H., Patel, A.P., Liu, G., Jhaveri, V.M., Si, S.K., Mittal, J., Bandstra, E.S., and Younis, R.T. (2017). Zika Virus: An Emerging Global Health Threat. Front. Cell. Infect. Microbiol., 7.","DOI":"10.3389\/fcimb.2017.00486"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Saba Villarroel, P.M., Hamel, R., Gumpangseth, N., Yainoy, S., Koomhin, P., Misse, D., and Wichit, S. (2024). Global seroprevalence of Zika virus in asymptomatic individuals: A systematic review. PLoS Neglected Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0011842"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e0103425","DOI":"10.1128\/spectrum.01034-25","article-title":"Zika virus impairs autophagic flux in trabecular meshwork, and inhibition of autophagy restricts ocular viral transmission and associated pathology","volume":"13","author":"Ahmad","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"ref_5","first-page":"e92340","article-title":"Zika virus infects cells lining the blood-retinal barrier and causes chorioretinal atrophy in mouse eyes","volume":"2","author":"Singh","year":"2017","journal-title":"J. Clin. Investig. Insight"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e10-1128","DOI":"10.1128\/mSphere.00173-19","article-title":"Zika Virus Infects Trabecular Meshwork and Causes Trabeculitis and Glaucomatous Pathology in Mouse Eyes","volume":"4","author":"Singh","year":"2019","journal-title":"mSphere"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1001\/jamaophthalmol.2016.0267","article-title":"Ocular Findings in Infants With Microcephaly Associated With Presumed Zika Virus Congenital Infection in Salvador, Brazil","volume":"134","author":"Prazeres","year":"2016","journal-title":"JAMA Ophthalmol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1001\/jamaophthalmol.2017.0561","article-title":"Ophthalmic Manifestations of Congenital Zika Syndrome in Colombia and Venezuela","volume":"135","author":"Yepez","year":"2017","journal-title":"JAMA Ophthalmol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1001\/jamaophthalmol.2016.4283","article-title":"Optical Coherence Tomography of Retinal Lesions in Infants With Congenital Zika Syndrome","volume":"134","author":"Ventura","year":"2016","journal-title":"JAMA Ophthalmol."},{"key":"ref_10","first-page":"e005161","article-title":"Corrigendum: Optic neuropathy and congenital glaucoma associated with probable Zika virus infection in Venezuelan patients","volume":"5","author":"Pettito","year":"2018","journal-title":"JMM Case Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e0055625","DOI":"10.1128\/jvi.00556-25","article-title":"Metabolic hijackers: How viral proteins redefine host cell landscapes","volume":"100","author":"Hafner","year":"2026","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yang, L.J., Tang, M., and Yang, L. (2026). Lipid metabolism, viral infection, and antiviral immunity: A new host-pathogen interface. Front. Cell. Infect. Microbiol., 16.","DOI":"10.3389\/fcimb.2026.1765502"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.molcel.2017.05.032","article-title":"AMPK: Mechanisms of Cellular Energy Sensing and Restoration of Metabolic Balance","volume":"66","author":"Garcia","year":"2017","journal-title":"Mol. Cell"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.cmet.2015.05.005","article-title":"Beyond energy homeostasis: The expanding role of AMP-activated protein kinase in regulating metabolism","volume":"21","author":"Carling","year":"2015","journal-title":"Cell Metab."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1074\/jbc.M116.763268","article-title":"AMP-activated Kinase (AMPK) Promotes Innate Immunity and Antiviral Defense through Modulation of Stimulator of Interferon Genes (STING) Signaling","volume":"292","author":"Prantner","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e0039425","DOI":"10.1128\/jvi.00394-25","article-title":"Direct pharmacological AMPK activation inhibits mucosal SARS-CoV-2 infection by reducing lipid metabolism, restoring autophagy flux and the type I IFN response","volume":"99","author":"Marteau","year":"2025","journal-title":"J. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bhutta, M.S., Gallo, E.S., and Borenstein, R. (2021). Multifaceted Role of AMPK in Viral Infections. Cells, 10.","DOI":"10.3390\/cells10051118"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.4049\/jimmunol.1901310","article-title":"AMP-Activated Protein Kinase Restricts Zika Virus Replication in Endothelial Cells by Potentiating Innate Antiviral Responses and Inhibiting Glycolysis","volume":"204","author":"Singh","year":"2020","journal-title":"J. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Prado, L.G., Camara, N.O.S., and Barbosa, A.S. (2023). Cell lipid biology in infections: An overview. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1148383"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41392-025-02357-x","article-title":"Lipid metabolism in homeostasis and disease","volume":"11","author":"Li","year":"2026","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e0395424","DOI":"10.1128\/mbio.03954-24","article-title":"Mammalian fatty acid synthase: A commonly used viral host dependency factor and a putative target for host-targeted broad-spectrum antiviral therapeutic development","volume":"16","author":"Karthigeyan","year":"2025","journal-title":"mBio"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhang, W., Xu, L., Zhu, L., Liu, Y., Yang, S., and Zhao, M. (2021). Lipid Droplets, the Central Hub Integrating Cell Metabolism and the Immune System. Front. Physiol., 12.","DOI":"10.3389\/fphys.2021.746749"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"105141","DOI":"10.1016\/j.micinf.2023.105141","article-title":"Controlling viral inflammatory lesions by inhibiting fatty acid metabolism","volume":"25","author":"Berber","year":"2023","journal-title":"Microbes Infect."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, J., Lan, Y., and Sanyal, S. (2017). Modulation of Lipid Droplet Metabolism-A Potential Target for Therapeutic Intervention in Flaviviridae Infections. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02286"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Pombo, J.P., and Sanyal, S. (2018). Perturbation of Intracellular Cholesterol and Fatty Acid Homeostasis During Flavivirus Infections. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.01276"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Camps, J., Iftimie, S., Jimenez-Franco, A., Castro, A., and Joven, J. (2025). Metabolic Reprogramming in Respiratory Viral Infections: A Focus on SARS-CoV-2, Influenza, and Respiratory Syncytial Virus. Biomolecules, 15.","DOI":"10.20944\/preprints202506.0375.v1"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e0182625","DOI":"10.1128\/jvi.01826-25","article-title":"Short-chain fatty acids butyrate and acetate limit Zika virus replication and associated ocular manifestations via the G-protein coupled receptor 43\/FFAR2","volume":"100","author":"Deshmukh","year":"2026","journal-title":"J. Virol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1097\/SHK.0000000000000730","article-title":"The AMPK Activator Aicar Ameliorates Age-Dependent Myocardial Injury in Murine Hemorrhagic Shock","volume":"47","author":"Matsiukevich","year":"2017","journal-title":"Shock"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"109337","DOI":"10.1016\/j.intimp.2022.109337","article-title":"Systemic administration of dorsomorphin relieves inflammatory nociception in the mouse formalin test","volume":"113","author":"Xinqiang","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s00430-024-00807-x","article-title":"Inhibiting lipid droplet biogenesis enhances host protection against hypervirulent Klebsiella pneumoniae infections","volume":"213","author":"Jung","year":"2024","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/srep00079","article-title":"Inhibition of fatty acid metabolism ameliorates disease activity in an animal model of multiple sclerosis","volume":"1","author":"Shriver","year":"2011","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e70004","DOI":"10.1111\/crj.70004","article-title":"Linoleic Acid Promotes Mitochondrial Biogenesis and Alleviates Acute Lung Injury","volume":"18","author":"Liu","year":"2024","journal-title":"Clin. Respir. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e2412895","DOI":"10.1002\/advs.202412895","article-title":"Palmitic Acid Accelerates Endothelial Cell Injury and Cardiovascular Dysfunction via Palmitoylation of PKM2","volume":"12","author":"He","year":"2025","journal-title":"Adv. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.jtos.2019.03.006","article-title":"Interferon-stimulated gene 15 (ISG15) restricts Zika virus replication in primary human corneal epithelial cells","volume":"17","author":"Singh","year":"2019","journal-title":"Ocul. Surf."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"109088","DOI":"10.1016\/j.isci.2024.109088","article-title":"Targeting ABCG1 and SREBP-2 mediated cholesterol homeostasis ameliorates Zika virus-induced ocular pathology","volume":"27","author":"Singh","year":"2024","journal-title":"iScience"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12974-023-02736-7","article-title":"Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells","volume":"20","author":"Dias","year":"2023","journal-title":"J. Neuroinflammation"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Samsa, M.M., Mondotte, J.A., Iglesias, N.G., Assuncao-Miranda, I., Barbosa-Lima, G., Da Poian, A.T., Bozza, P.T., and Gamarnik, A.V. (2009). Dengue virus capsid protein usurps lipid droplets for viral particle formation. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000632"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Hsia, J.Z., Liu, D., Haynes, L., Cruz-Cosme, R., and Tang, Q. (2024). Lipid Droplets: Formation, Degradation, and Their Role in Cellular Responses to Flavivirus Infections. Microorganisms, 12.","DOI":"10.3390\/microorganisms12040647"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Dias, S.S.G., Soares, V.C., Ferreira, A.C., Sacramento, C.Q., Fintelman-Rodrigues, N., Temerozo, J.R., Teixeira, L., Nunes da Silva, M.A., Barreto, E., and Mattos, M. (2020). Lipid droplets fuel SARS-CoV-2 replication and production of inflammatory mediators. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1009127"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1038\/nm.2238","article-title":"Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1","volume":"16","author":"Herker","year":"2010","journal-title":"Nat. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1099\/vir.0.050146-0","article-title":"Inhibition of rotavirus replication by downregulation of fatty acid synthesis","volume":"94","author":"Gaunt","year":"2013","journal-title":"J. Gen. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.chom.2010.10.006","article-title":"Dengue virus-induced autophagy regulates lipid metabolism","volume":"8","author":"Heaton","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.theriogenology.2012.11.025","article-title":"Linoleic acid stimulates neutral lipid accumulation in lipid droplets of maturing bovine oocytes","volume":"79","author":"Carro","year":"2013","journal-title":"Theriogenology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"146484","DOI":"10.1016\/j.brainres.2019.146484","article-title":"Oleic acid is a potent inducer for lipid droplet accumulation through its esterification to glycerol by diacylglycerol acyltransferase in primary cortical astrocytes","volume":"1725","author":"Nakajima","year":"2019","journal-title":"Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/s42003-024-06248-9","article-title":"Palmitic acid induces lipid droplet accumulation and senescence in nucleus pulposus cells via ER-stress pathway","volume":"7","author":"Chen","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Soto-Acosta, R., Bautista-Carbajal, P., Cervantes-Salazar, M., Angel-Ambrocio, A.H., and Del Angel, R.M. (2017). DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006257"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s12944-019-1136-y","article-title":"Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK\/SREBP-1c signaling","volume":"18","author":"Meng","year":"2019","journal-title":"Lipids Health Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"105666","DOI":"10.1016\/j.antiviral.2023.105666","article-title":"Identification of alpha-linolenic acid as a broad-spectrum antiviral against zika, dengue, herpes simplex, influenza virus and SARS-CoV-2 infection","volume":"216","author":"Feng","year":"2023","journal-title":"Antivir. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5207","DOI":"10.1038\/s41598-021-84850-1","article-title":"Polyunsaturated omega-3 fatty acids inhibit ACE2-controlled SARS-CoV-2 binding and cellular entry","volume":"11","author":"Goc","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/BF01320626","article-title":"Unsaturated free fatty acids inactivate animal enveloped viruses","volume":"66","author":"Kohn","year":"1980","journal-title":"Arch. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Lampova, B., Doskocil, I., Kourimska, L., and Kopec, A. (2022). N-3 polyunsaturated fatty acids may affect the course of COVID-19. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.957518"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"106364","DOI":"10.1016\/j.antiviral.2026.106364","article-title":"Targeting the fatty acid metabolism pathway represents a promising antiviral strategy against diverse viral infections","volume":"248","author":"Liu","year":"2026","journal-title":"Antivir. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1038\/s42255-021-00479-4","article-title":"Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication","volume":"3","author":"Chu","year":"2021","journal-title":"Nat. Metab."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ohol, Y.M., Wang, Z., Kemble, G., and Duke, G. (2015). Direct Inhibition of Cellular Fatty Acid Synthase Impairs Replication of Respiratory Syncytial Virus and Other Respiratory Viruses. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0144648"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"109829","DOI":"10.1016\/j.fsi.2024.109829","article-title":"Involvement of mitochondrial fatty acid beta-oxidation in the antiviral innate immune response in head kidney macrophages of large yellow croaker (Larimichthys crocea)","volume":"153","author":"Liu","year":"2024","journal-title":"Fish Shellfish. Immunol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/9\/817\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:02:06Z","timestamp":1777546926000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/9\/817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":55,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["cells15090817"],"URL":"https:\/\/doi.org\/10.3390\/cells15090817","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]}}}