{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:13:45Z","timestamp":1791922425617,"version":"4.3.4"},"reference-count":83,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Cynthia Van Rompay","doi-asserted-by":"publisher","award":["1S10525N"],"award-info":[{"award-number":["1S10525N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012331","name":"VLAIO","doi-asserted-by":"publisher","award":["HBC.2022.0569"],"award-info":[{"award-number":["HBC.2022.0569"]}],"id":[{"id":"10.13039\/100012331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Three-dimensional (3D) cardiac models, including spheroids, organoids, and organ-on-chips, are advanced systems for studying human physiology, disease, and drug responses with greater biological relevance than 2D models. As their use expands in biomedical research, tissue engineering, and regenerative medicine, reliable preservation methods are needed. However, cryopreservation often fails to protect 3D systems due to limited cryoprotectant penetration, ice formation, and mechanical stress during freezing and thawing. Room-temperature (RT) preservation has emerged as a promising alternative for short-term transport. This study evaluated a RT-based transport medium (CellShip\u00ae) for preserving cardiac organoids for up to seven days, compared with conventional cryopreservation using slow-freezing in Cryostor\u00aeCS10. Viability and functionality were assessed using apoptosis, ATP levels, beating activity, proliferation, and size. During maturation, organoids showed increased size, ATP levels, and beating capacity. Cryopreservation reduced size, proliferation, ATP levels, and altered beating, while increasing apoptosis. In contrast, RT preservation maintained stable viability and functionality after recovery. These findings demonstrate that RT preservation effectively maintains cardiac organoid integrity and function, offering a promising alternative for short-term storage and transport, with potential terrestrial and nonterrestrial applications.<\/jats:p>","DOI":"10.3390\/cells15121065","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:57:17Z","timestamp":1781161037000},"page":"1065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of a Room-Temperature Preservation Method Maintaining Viability and Function in Human Cardiac Organoids"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0283-3204","authenticated-orcid":false,"given":"Cynthia","family":"Van Rompay","sequence":"first","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"},{"name":"Biomimetics Group, Department of Biosystems, Division Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8004-3718","authenticated-orcid":false,"given":"Kevin","family":"Tabury","sequence":"additional","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"},{"name":"Department of Biomedical Engineering, College of Engineering and Computing, University of South Carolina, Columbia, SC 29208, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5318-5139","authenticated-orcid":false,"given":"Emil","family":"Rehnberg","sequence":"additional","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"},{"name":"Department of Physics and Astronomy, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00eb","family":"Janssen","sequence":"additional","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8110-751X","authenticated-orcid":false,"given":"Sarah","family":"Baatout","sequence":"additional","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"},{"name":"Department of Physics and Astronomy, Faculty of Sciences, Ghent University, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8263-0350","authenticated-orcid":false,"given":"Marianne S.","family":"Carlon","sequence":"additional","affiliation":[{"name":"Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), Department of Chronic Diseases and Metabolism, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier Casadevall i","family":"Solvas","sequence":"additional","affiliation":[{"name":"Biomimetics Group, Department of Biosystems, Division Mechatronics, Biostatistics and Sensors (MeBioS), KU Leuven, 3001 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9840-2822","authenticated-orcid":false,"given":"Bjorn","family":"Baselet","sequence":"additional","affiliation":[{"name":"Nuclear Medical Applications, Radiobiology Unit, Belgian Nuclear Research Centre SCK CEN, 2400 Mol, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yaqinuddin, A., Jabri, A., Mhannayeh, A., Taftafa, B., Alsharif, M., Abbad, T., Khan, J., Elsalti, A., Chinnappan, R., and Alshehri, E.A. 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