{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:59:08Z","timestamp":1785405548363,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Save Sight Institute, the University of Sydney"},{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}]},{"name":"private family foundation"},{"name":"University of Delaware, USA"},{"id":[{"id":"https:\/\/ror.org\/01sbq1a82","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Transforming Growth Factor-beta (TGF-\u03b2) promotes lens epithelial\u2013mesenchymal transition (EMT) and fibrosis, contributing to anterior subcapsular cataract (ASC) formation. Transgenic mice overexpressing TGF-\u03b21 in the lens have been studied for over three decades, and yet the impact of active TGF-\u03b21-overexpression on the lens epithelial transcriptome is undefined. We have addressed this knowledge gap by examining the gene expression landscape of these unique lens epithelia. High-throughput RNA-sequencing was performed on isolated lens epithelia from three-week-old TGF-\u03b21-overexpression transgenic mice from two independent lines, OVE853 and OVE918, and wild-type mice. Downstream analyses included comparisons with lens datasets (e.g., cataract surgery model) and investigations using various resources\/tools (e.g., Gene Ontology, CompBio, and iSyTE). Compared to wild-type murine lens epithelia, 384 differentially expressed genes (DEGs) were commonly identified in the lens of both transgenic lines. Candidates involved in EMT, inflammatory response, extracellular matrix organization, and mechano-sensation were elevated, while those involved in lipid metabolism, Wnt-suppression, Bmp- and Notch-activation were reduced. Comparative analyses with temporal transcriptomes on a mouse cataract surgery model identified overlapping pathological pathways, and some elevated genes, for example, endoplasmic reticulum stress genes, were consistent with human ASC data. This study provides the first comprehensive transcriptomic characterization of two independent TGF-\u03b21 transgenic ASC models and identifies novel candidate downstream genes and pathways associated with TGF-\u03b21 overexpression. All our data is made user-friendly and accessible through iSyTE.<\/jats:p>","DOI":"10.3390\/cells15141263","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T08:04:31Z","timestamp":1784016271000},"page":"1263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome Changes Driving Multiple Regulatory Pathways Involved in TGF-\u03b2-Induced Anterior Subcapsular Cataract"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6023-0235","authenticated-orcid":false,"given":"Sarah Y.","family":"Coomson","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4873-2385","authenticated-orcid":false,"given":"Chirag","family":"Parsania","sequence":"additional","affiliation":[{"name":"Cancer & Gene Regulation Laboratory, Centenary Institute, Sydney, NSW 2006, Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4725-7829","authenticated-orcid":false,"given":"Charles G.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Cancer & Gene Regulation Laboratory, Centenary Institute, Sydney, NSW 2006, Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Metierre","sequence":"additional","affiliation":[{"name":"Cancer & Gene Regulation Laboratory, Centenary Institute, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-4511","authenticated-orcid":false,"given":"Mary","family":"Flokis","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8845-010X","authenticated-orcid":false,"given":"Salil A.","family":"Lachke","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA"},{"name":"Center for Bioinformatics and Computational Biology, University of Delaware, Newark, DE 19716, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5492-9200","authenticated-orcid":false,"given":"Frank J.","family":"Lovicu","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia"},{"name":"Save Sight Institute, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1159\/000084508","article-title":"Transforming Growth Factor-Beta-Induced Epithelial-Mesenchymal Transition in the Lens: A Model for Cataract Formation","volume":"179","author":"Wederell","year":"2005","journal-title":"Cells Tissues Organs"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1016\/j.ajpath.2014.03.013","article-title":"Matrix Metalloproteinase-9\u2013Null Mice Are Resistant to TGF-\u03b2\u2013Induced Anterior Subcapsular Cataract Formation","volume":"184","author":"Korol","year":"2014","journal-title":"Am. J. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8362","DOI":"10.1038\/srep08362","article-title":"Quantitative Analysis of Injury-Induced Anterior Subcapsular Cataract in the Mouse: A Model of Lens Epithelial Cells Proliferation and Epithelial-Mesenchymal Transition","volume":"5","author":"Xiao","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s10911-010-9181-1","article-title":"The Pathophysiology of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor-\u03b2 in Normal and Malignant Mammary Epithelial Cells","volume":"15","author":"Taylor","year":"2010","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kubo, E., Shibata, T., Singh, D.P., and Sasaki, H. (2018). Roles of TGF \u03b2 and FGF Signals in the Lens: Tropomyosin Regulation for Posterior Capsule Opacity. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19103093"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1136\/bjo.86.2.220","article-title":"TGF\u03b2 Induces Morphological and Molecular Changes Similar to Human Anterior Subcapsular Cataract","volume":"86","author":"Lovicu","year":"2002","journal-title":"Br. J. Ophthalmol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3450","DOI":"10.1167\/iovs.05-1208","article-title":"Lens-Specific Expression of TGF-Beta Induces Anterior Subcapsular Cataract Formation in the Absence of Smad3","volume":"47","author":"Banh","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"69","DOI":"10.2353\/ajpath.2006.041089","article-title":"Matrix Metalloproteinase Inhibitors Suppress Transforming Growth Factor-\u03b2-Induced Subcapsular Cataract Formation","volume":"168","author":"Dwivedi","year":"2006","journal-title":"Am. J. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"110585","DOI":"10.1016\/j.exer.2025.110585","article-title":"Posterior Capsular Opacification: Pathogenesis, Challenges, and Innovative Therapeutic Strategies","volume":"259","author":"Wang","year":"2025","journal-title":"Exp. Eye Res."},{"key":"ref_10","first-page":"3094","article-title":"Propagation and Immortalization of Human Lens Epithelial Cells in Culture","volume":"35","author":"Andley","year":"1994","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.exer.2015.04.021","article-title":"Experimental Models for Posterior Capsule Opacification Research","volume":"142","author":"Wormstone","year":"2016","journal-title":"Exp. Eye Res."},{"key":"ref_12","first-page":"102522","article-title":"Crucial Involvement of Transforming Growth Factor Beta (TGF-\u03b2)\/Smad Signaling Regulation by miR-204-5p in Cataract and Posterior Capsular Opacification","volume":"45","author":"Sadeghi","year":"2026","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"376","DOI":"10.2741\/s32","article-title":"TGF Beta in Fibroproliferative Diseases in the Eye","volume":"1","author":"Saika","year":"2009","journal-title":"Front. Biosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"149338","DOI":"10.1016\/j.gene.2025.149338","article-title":"Crosstalk between Signaling Pathways (Rho\/ROCK, TGF-\u03b2 and Wnt\/\u03b2-Catenin Pathways\/PI3K-AKT-mTOR) in Cataract: A Mechanistic Exploration and Therapeutic Strategy","volume":"947","author":"Khan","year":"2025","journal-title":"Gene"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Taiyab, A., Belahlou, Y., Wong, V., Pandi, S., Shekhar, M., Chidambaranathan, G.P., and West-Mays, J. (2023). Understanding the Role of Yes-Associated Protein (YAP) Signaling in the Transformation of Lens Epithelial Cells (EMT) and Fibrosis. Biomolecules, 13.","DOI":"10.3390\/biom13121767"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1172\/JCI1360","article-title":"Lens-Specific Expression of Transforming Growth Factor Beta1 in Transgenic Mice Causes Anterior Subcapsular Cataracts","volume":"101","author":"Srinivasan","year":"1998","journal-title":"J. Clin. Investig."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Siddam, A.D., Duot, M., Coomson, S.Y., Anand, D., Aryal, S., Weatherbee, B.A.T., Audic, Y., Paillard, L., and Lachke, S.A. (2023). High-Throughput Transcriptomics of Celf1 Conditional Knockout Lens Identifies Downstream Networks Linked to Cataract Pathology. Cells, 12.","DOI":"10.3390\/cells12071070"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes Adapter Sequences from High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet.journal"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast Universal RNA-Seq Aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinforma. Oxf. Engl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: An Efficient General Purpose Program for Assigning Sequence Reads to Genomic Features","volume":"30","author":"Liao","year":"2014","journal-title":"Bioinforma. Oxf. Engl."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1186\/gb-2010-11-3-r25","article-title":"A Scaling Normalization Method for Differential Expression Analysis of RNA-Seq Data","volume":"11","author":"Robinson","year":"2010","journal-title":"Genome Biol."},{"key":"ref_22","first-page":"1438","article-title":"From Reads to Genes to Pathways: Differential Expression Analysis of RNA-Seq Experiments Using Rsubread and the edgeR Quasi-Likelihood Pipeline","volume":"5","author":"Chen","year":"2016","journal-title":"F1000Research"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"D875","DOI":"10.1093\/nar\/gkx837","article-title":"iSyTE 2.0: A Database for Expression-Based Gene Discovery in the Eye","volume":"46","author":"Kakrana","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s00439-018-1958-0","article-title":"RNA Sequencing-Based Transcriptomic Profiles of Embryonic Lens Development for Cataract Gene Discovery","volume":"137","author":"Anand","year":"2018","journal-title":"Hum. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1167\/iovs.66.4.42","article-title":"Transcriptome Meta-Analysis Uncovers Cell-Specific Regulatory Relationships in Embryonic, Juvenile, Adult, and Aged Mouse Lens Epithelium and Fibers","volume":"66","author":"Duot","year":"2025","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"clusterProfiler: An R Package for Comparing Biological Themes among Gene Clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics J. Integr. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cels.2015.12.004","article-title":"The Molecular Signatures Database (MSigDB) Hallmark Gene Set Collection","volume":"1","author":"Liberzon","year":"2015","journal-title":"Cell Syst."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1038\/s41592-023-02014-7","article-title":"Extending Support for Mouse Data in the Molecular Signatures Database (MSigDB)","volume":"20","author":"Castanza","year":"2023","journal-title":"Nat. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Novo, S.G., Faranda, A.P., Shihan, M.H., Wang, Y., Garg, A., and Duncan, M.K. (2022). The Immediate Early Response of Lens Epithelial Cells to Lens Injury. Cells, 11.","DOI":"10.3390\/cells11213456"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4986","DOI":"10.1167\/iovs.18-25067","article-title":"Lens Epithelial Cells Initiate an Inflammatory Response Following Cataract Surgery","volume":"59","author":"Jiang","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.matbio.2020.02.004","article-title":"Fibronectin Has Multifunctional Roles in Posterior Capsular Opacification (PCO)","volume":"90","author":"Shihan","year":"2020","journal-title":"Matrix Biol. J. Int. Soc. Matrix Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1167\/iovs.66.9.53","article-title":"LIRTS Viewer: A Web-Based Resource to View the Transcriptional Response of Lens Epithelial Cells to Injury","volume":"66","author":"Gorai","year":"2025","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and Integrative Analysis of Large Gene Lists Using DAVID Bioinformatics Resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: A Web Server for Functional Enrichment Analysis and Functional Annotation of Gene Lists (2021 Update)","volume":"50","author":"Sherman","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1080\/00949658508810839","article-title":"Computer Generation of Hypergeometric Random Variates\u2020","volume":"22","author":"Kachitvichyanukul","year":"1985","journal-title":"J. Stat. Comput. Simul."},{"key":"ref_36","first-page":"348","article-title":"Human Lens Epithelial Cells Induce the Inflammatory Response When Placed into the Lens Capsular Bag Model of Posterior Capsular Opacification","volume":"30","author":"Novo","year":"2024","journal-title":"Mol. Vis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Barve, R.A., Storer, C.E., Hoxsie, W.D., Marcum, C., McMichael, J.F., Lalmansingh, J.M., Smith, B.K., Johnson, M.R., Kuster, D.J., and Head, R.D. (2025). CompBio and MIRaS\u2014A Multi-Omic Analysis Platform Built on a Memory-Based Intelligence Engine. bioRxiv, bioRxiv:2025.12.11.693741.","DOI":"10.64898\/2025.12.11.693741"},{"key":"ref_38","first-page":"35","article-title":"PERK Regulates Epithelial-Mesenchymal Transition Through Autophagy and Lipid Metabolism in Lens Epithelial Cells","volume":"66","author":"Wang","year":"2025","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1086\/346090","article-title":"Connexin 43 (GJA1) Mutations Cause the Pleiotropic Phenotype of Oculodentodigital Dysplasia","volume":"72","author":"Paznekas","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"ref_40","first-page":"309","article-title":"Heterozygous GJA1 Variants with Ocular Phenotype: Missense in Domain but Truncation out of Domain","volume":"27","author":"Li","year":"2021","journal-title":"Mol. Vis."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"108546","DOI":"10.1016\/j.exer.2021.108546","article-title":"Contrasting Roles for BMP-4 and Ventromorphins (BMP Agonists) in TGF\u03b2-Induced Lens EMT","volume":"206","author":"Shu","year":"2021","journal-title":"Exp. Eye Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1167\/iovs.64.4.12","article-title":"BMP-4 and BMP-7 Inhibit EMT in a Model of Anterior Subcapsular Cataract in Part by Regulating the Notch Signaling Pathway","volume":"64","author":"Jiang","year":"2023","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Rakib-Uz-Zaman, S.M., Werner, L., and Duncan, M.K. (2025). Unanswered Questions Regarding the Pathogenesis of Late Onset Posterior Capsular Opacification. Front. Ophthalmol., 5.","DOI":"10.3389\/fopht.2025.1680042"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1136\/bjo.84.3.332","article-title":"Role of Cytokines in the Pathogenesis of Posterior Capsule Opacification","volume":"84","author":"Meacock","year":"2000","journal-title":"Br. J. Ophthalmol."},{"key":"ref_45","first-page":"2301","article-title":"TGF-Beta2-Induced Matrix Modification and Cell Transdifferentiation in the Human Lens Capsular Bag","volume":"43","author":"Wormstone","year":"2002","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"O\u2019Neill, L.M., Wang, Y., and Duncan, M.K. (2023). Modeling Cataract Surgery in Mice. J. Vis. Exp. JoVE.","DOI":"10.3791\/66050"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jtos.2013.12.002","article-title":"Clinical and Genetic Aspects of the TGFBI-Associated Corneal Dystrophies","volume":"12","author":"Lakshminarayanan","year":"2014","journal-title":"Ocul. Surf."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/s42003-026-09601-2","article-title":"TGFBI Promotes Liver Fibrosis through Remodeling the Profibrotic Microenvironment by a Positive Feedback Regulatory Loop","volume":"9","author":"Wu","year":"2026","journal-title":"Commun. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e70112","DOI":"10.1111\/cpr.70112","article-title":"Piezo2 in Mechanosensory Biology: From Physiological Homeostasis to Disease-Promoting Mechanisms","volume":"59","author":"Cheng","year":"2026","journal-title":"Cell Prolif."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e69626","DOI":"10.7554\/eLife.69626","article-title":"Inhibition of Itch by Neurokinin 1 Receptor (Tacr1)-Expressing ON Cells in the Rostral Ventromedial Medulla in Mice","volume":"11","author":"Follansbee","year":"2022","journal-title":"eLife"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Imanaka-Yoshida, K., and Aoki, H. (2014). Tenascin-C and Mechanotransduction in the Development and Diseases of Cardiovascular System. Front. Physiol., 5.","DOI":"10.3389\/fphys.2014.00283"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3073","DOI":"10.1523\/JNEUROSCI.3699-14.2015","article-title":"The PDZ-Domain Protein Whirlin Facilitates Mechanosensory Signaling in Mammalian Proprioceptors","volume":"35","author":"Simon","year":"2015","journal-title":"J. Neurosci. Off. J. Soc. Neurosci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/jcmm.12213","article-title":"The Roles of \u03b1V Integrins in Lens EMT and Posterior Capsular Opacification","volume":"18","author":"Mamuya","year":"2014","journal-title":"J. Cell. Mol. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"111042","DOI":"10.1016\/j.exer.2026.111042","article-title":"ITGA11 Regulates Lens Epithelial-Mesenchymal Transition by Modulating PTGS2-Mediated Lipid Metabolism","volume":"268","author":"Wang","year":"2026","journal-title":"Exp. Eye Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.ydbio.2012.05.022","article-title":"Parallels between Neuron and Lens Fiber Cell Structure and Molecular Regulatory Networks","volume":"368","author":"Frederikse","year":"2012","journal-title":"Dev. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1007\/s11064-016-2156-x","article-title":"Lens Biology Is a Dimension of Neurobiology","volume":"42","author":"Frederikse","year":"2017","journal-title":"Neurochem. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1038\/s41598-021-81611-y","article-title":"Absence of S100A4 in the Mouse Lens Induces an Aberrant Retina-Specific Differentiation Program and Cataract","volume":"11","author":"Maddala","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1167\/iovs.65.4.42","article-title":"miR-26 Deficiency Causes Alterations in Lens Transcriptome and Results in Adult-Onset Cataract","volume":"65","author":"Upreti","year":"2024","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fan, C., Wang, C., Wang, Y., and Jiang, J. (2024). Transcriptome Exploration of Ferroptosis-Related Genes in TGF\u03b2- Induced Lens Epithelial to Mesenchymal Transition during Posterior Capsular Opacification Development. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10244-y"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1167\/iovs.64.13.37","article-title":"Single-Cell RNA Sequencing Analysis of the Early Postnatal Mouse Lens Epithelium","volume":"64","author":"Giannone","year":"2023","journal-title":"Investig. Ophthalmol. Vis. Sci."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/14\/1263\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T08:11:32Z","timestamp":1784016692000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/14\/1263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":60,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["cells15141263"],"URL":"https:\/\/doi.org\/10.3390\/cells15141263","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]}}}