{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T05:01:08Z","timestamp":1784869268382,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016692","name":"Key Research and Development Program of Ningxia","doi-asserted-by":"publisher","award":["2023BEG03053"],"award-info":[{"award-number":["2023BEG03053"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016692","name":"Key Research and Development Program of Ningxia","doi-asserted-by":"publisher","award":["2026BBF02008"],"award-info":[{"award-number":["2026BBF02008"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["32460172"],"award-info":[{"award-number":["32460172"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"crossref","award":["2026AAC020016"],"award-info":[{"award-number":["2026AAC020016"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>LncRNA NR_003508 has been implicated in lipopolysaccharide (LPS)-induced acute respiratory distress syndrome. Yet, the involvement of LncRNA NR_003508 in Pseudomonas aeruginosa (PA)-induced autophagy remains elusive. This study aims to reveal the underlying mechanisms of LncRNA NR_003508 in PA-induced autophagy, thereby enhancing the basic research content of the pathogenesis of PA infection. Fluorescence in situ hybridization (FISH) probes and q-PCR revealed that PA infection induced macrophage autophagy accompanied by increasing LncRNA NR_003508, suggesting that LncRNA NR_003508 may participate in competing endogenous RNA (ceRNA)-mediated regulatory networks. Concurrently, autophagic clearance of PA requires LncRNA NR_003508. Then, we used the Gene Expression Omnibus (GEO) database to find that LC3 was considered a core protein in PA-infected autophagy. Furthermore, LncRNA NR_003508 promoted the formation of autophagosomes, which played a positive role in PA clearance. Functionally, bioinformatics analysis and luciferase reporter assays demonstrated that LncRNA NR_003508 promotes the conversion of LC3I to LC3II by sponging miR-344i and facilitates p62 degradation by binding to miR-344g-5p. Taken together, our data established that LncRNA NR_003508 enhanced the formation of autophagosomes induced by PA infection via the miR-344i\/LC3 and miR-344g-5p\/p62 axes, partly boosting the clearance of PA and providing a theoretical basis and experimental support for understanding the mechanisms underlying PA infection.<\/jats:p>","DOI":"10.3390\/cells15141306","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"1306","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LncRNA NR_003508 Boosts Pseudomonas aeruginosa-Induced Autophagy by Facilitating the Conversion of LC3-I to LC3-II and p62 Degradation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4388-7778","authenticated-orcid":false,"given":"Li","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Science, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8812-0905","authenticated-orcid":false,"given":"Jiaxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Science, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangcun","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Life Science, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6390-3528","authenticated-orcid":false,"given":"Xiaoling","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Science, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tuon, F.F., Dantas, L.R., Suss, P.H., and Tasca Ribeiro, V.S. 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