{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T19:01:32Z","timestamp":1785438092939,"version":"3.56.0"},"reference-count":49,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"ICMR","doi-asserted-by":"publisher","award":["Ref # VBD\/NIMR\/Intra\/002-ECD-II"],"award-info":[{"award-number":["Ref # VBD\/NIMR\/Intra\/002-ECD-II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Ref # VBD\/NIMR\/Intra\/002-ECD-II"],"award-info":[{"award-number":["Ref # VBD\/NIMR\/Intra\/002-ECD-II"]}],"id":[{"id":"https:\/\/ror.org\/0492wrx28","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"UGC","doi-asserted-by":"publisher","award":["Ref # 191620022266"],"award-info":[{"award-number":["Ref # 191620022266"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]},{"award":["Ref # 191620022266"],"award-info":[{"award-number":["Ref # 191620022266"]}],"id":[{"id":"https:\/\/ror.org\/04p800546","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Our study investigated how adult female Anopheles culicifacies mosquitoes regulate systemic iron homeostasis after blood feeding, a process essential for reproduction, and revealed striking parallels to iron deficiency disorders in mammals. This study identifies that coordinated transcriptional regulation of ferritin and transferrin plays a crucial role in follicle development and egg maturation. Silencing of both genes using ribonucleic acid interference led to severe reproductive impairment, including ovarian arrest in 50% of females, a 40% reduction in oocyte number, and a decrease in first instar larval size. These outcomes correlate with increased reactive oxygen species and altered serotonin receptor expression in the brain, possibly driven by alterations in microbial gut\u2013brain axis communication due to disrupted iron metabolism. In summary, our research provides the first molecular proof and a new conceptual understanding of how iron metabolism disorders may affect microbiome\u2013gut\u2013brain-axis communication and, in turn, reproductive outcomes.<\/jats:p>","DOI":"10.3390\/cells15151315","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:11:42Z","timestamp":1784787102000},"page":"1315","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Iron Homeostasis and Reproduction: Unveiling the Microbiome\u2013Gut\u2013Brain Axis Connection in the Mosquito Anopheles culicifacies"],"prefix":"10.3390","volume":"15","author":[{"given":"Pooja","family":"Yadav","sequence":"first","affiliation":[{"name":"Laboratory of Host\u2013Parasite Interaction Studies, Department of Vector Genomics, ICMR\u2014National Institute of Malaria Research, Dwarka, New Delhi 110077, India"},{"name":"Academy of Scientific and 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Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0378-1119(01)00602-3","article-title":"Transcriptional regulation of the mosquito vitellogenin gene via a blood meal-triggered cascade","volume":"274","author":"Kokoza","year":"2001","journal-title":"Gene"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ibmb.2005.02.013","article-title":"Nutritional regulation of vitellogenesis in mosquitoes: Implications for anautogeny","volume":"35","author":"Attardo","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Das De, T., Sharma, P., Tevatiya, S., Chauhan, C., Kumari, S., Yadav, P., and Dixit, R. (2022). Bidirectional microbiome-gut-brain-axis communication influences metabolic switch-associated responses in the mosquito Anopheles culicifacies. Cells, 11.","DOI":"10.3390\/cells11111798"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Whiten, S.R., Eggleston, H., and Adelman, Z.N. (2018). Ironing out the details: Exploring the role of iron and heme in blood-sucking arthropods. Front. Physiol., 8.","DOI":"10.3389\/fphys.2017.01134"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100965","DOI":"10.1016\/j.cois.2022.100965","article-title":"Metal ions in the physiology of insects","volume":"54","author":"Missirlis","year":"2022","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1146\/annurev.ento.47.091201.145237","article-title":"Iron metabolism in insects","volume":"47","author":"Nichol","year":"2002","journal-title":"Annu. Rev. Entomol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"26729","DOI":"10.1074\/jbc.R110.113217","article-title":"Redox cycling in iron uptake, efflux, and trafficking","volume":"285","author":"Kosman","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-3-030-62026-4_3","article-title":"Iron homeostasis and metabolism: Two sides of a coin","volume":"1301","author":"Venkataramani","year":"2021","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_12","first-page":"229","article-title":"Iron: Effect of overload and deficiency","volume":"13","author":"Hider","year":"2013","journal-title":"Met. Ions Life Sci."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Arosio, P. (2022). New advances in iron metabolism, ferritin and hepcidin research. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232314700"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1146\/annurev-ento-040622-092836","article-title":"Iron homeostasis in insects","volume":"68","author":"Gorman","year":"2023","journal-title":"Annu. Rev. Entomol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Talyuli, O.A.C. (2023). The Aedes aegypti peritrophic matrix controls arbovirus vector competence through HPx1, a heme\u2013induced peroxidase. PLoS Pathog., 19.","DOI":"10.1371\/journal.ppat.1011149"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8030","DOI":"10.1073\/pnas.0602224103","article-title":"A heme-degradation pathway in a blood-sucking insect","volume":"103","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Walter-Nuno, A.B., Taracena, M.L., Mesquita, R.D., Oliveira, P.L., and Paiva-Silva, G.O. (2018). Silencing of iron and heme-related genes revealed a paramount role of iron in the physiology of the hematophagous vector Rhodnius prolixus. Front. Genet., 9.","DOI":"10.3389\/fgene.2018.00019"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1016\/j.bbamcr.2012.03.004","article-title":"Ferroportin-mediated iron transport: Expression and regulation","volume":"1823","author":"Ward","year":"2012","journal-title":"Biochim. Biophys. Acta (BBA)-Mol. Cell Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1073\/pnas.0807961106","article-title":"Knockdown of proteins involved in iron metabolism limits tick reproduction and development","volume":"106","author":"Hajdusek","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.jinsphys.2007.06.009","article-title":"Fate of blood meal iron in mosquitoes","volume":"53","author":"Zhou","year":"2007","journal-title":"J. Insect Physiol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rani, J., De, T.D., Chauhan, C., Kumari, S., Sharma, P., Tevatiya, S., Chakraborti, S., Pandey, K.C., Singh, N., and Dixit, R. (2022). Functional disruption of transferrin expression alters reproductive physiology in Anopheles culicifacies. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0264523"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s12011-018-1442-7","article-title":"Iron deficiency reduces synapse formation in the Drosophila clock circuit","volume":"189","author":"Rudisill","year":"2019","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00361","DOI":"10.1016\/j.heliyon.2017.e00361","article-title":"Sex specific molecular responses of quick-to-court protein in Indian malarial vector Anopheles culicifacies: Conflict of mating versus blood feeding behaviour","volume":"3","author":"Tevatiya","year":"2017","journal-title":"Heliyon"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43618","DOI":"10.1038\/srep43618","article-title":"Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti","volume":"7","author":"Dzaki","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ahmed, T.H., Saunders, T.R., Mullins, D., Rahman, M.Z., and Zhu, J. (2020). Molecular action of pyriproxyfen: Role of the methoprene-tolerant protein in the pyriproxyfen-induced sterilization of adult female mosquitoes. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008669"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1038\/s41598-022-06531-x","article-title":"A testis-expressing heme peroxidase HPX12 regulates male fertility in the mosquito Anopheles stephensi","volume":"12","author":"Kumari","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Teufel, M., and Sobetzko, P. (2022). Reducing costs for DNA and RNA sequencing by sample pooling using a metagenomic approach. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08831-y"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Clausen, P.T.L.C., Aarestrup, F.M., and Lund, O. (2018). Rapid and precise alignment of raw reads against redundant databases with KMA. BMC Bioinform., 19.","DOI":"10.1186\/s12859-018-2336-6"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: An ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1101\/gr.228429.117","article-title":"Genome-reconstruction for eukaryotes from complex natural microbial communities","volume":"28","author":"West","year":"2018","journal-title":"Genome Res."},{"key":"ref_32","first-page":"50892","article-title":"Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response","volume":"82","author":"Ajjuri","year":"2013","journal-title":"J. Vis. Exp."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/S0965-1748(03)00124-3","article-title":"Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti","volume":"33","author":"Sanders","year":"2003","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"13726","DOI":"10.1038\/s41598-019-49396-3","article-title":"Non-canonical transcriptional regulation of heme oxygenase in Aedes aegypti","volume":"9","author":"Pereira","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1017\/S0029665113003637","article-title":"Iron deficiency during pregnancy: The consequences for placental function and fetal outcome","volume":"73","author":"McArdle","year":"2014","journal-title":"Proc. Nutr. Soc."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Sonawane, M.S., Salunkhe, R.C., and Sayyed, R.Z. (2021). Insect gut bacteria and iron metabolism in insects. Probiotics, the Natural Microbiota in Living Organisms, CRC Press.","DOI":"10.1201\/9781351027540-13"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Chen, S., Johnson, B.K., Yu, T., Nelson, B.N., and Walker, E.D. (2020). Elizabethkingia anophelis: Physiologic and transcriptomic responses to iron stress. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00804"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"He, S., and Qi, Y. (2025). The microbiota, the malarial parasite, and the mice\u2014A three-sided relationship. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1615846"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e41617","DOI":"10.1097\/MD.0000000000041617","article-title":"Gut microbiota and risk of iron deficiency anemia: A two-sample Mendelian randomization study","volume":"104","author":"Lei","year":"2025","journal-title":"Medicine"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Malesza, I.J., Bartkowiak-Wieczorek, J., Winkler-Galicki, J., Nowicka, A., Dzi\u0119cio\u0142owska, D., B\u0142aszczyk, M., Gajniak, P., S\u0142owi\u0144ska, K., Niepolski, L., and Walkowiak, J. (2022). The dark side of iron: The relationship between iron, inflammation and gut microbiota in selected diseases associated with iron deficiency anaemia\u2014A narrative review. Nutrients, 14.","DOI":"10.3390\/nu14173478"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Bao, H., Wang, Y., Xiong, H., Xia, Y., Cui, Z., and Liu, L. (2024). Mechanism of iron ion homeostasis in intestinal immunity and gut microbiota remodeling. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25020727"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Medeiros, M.S., Schumacher-Schuh, A., Cardoso, A.M., Rieder, C.R., and Fighera, M.R. (2016). Iron and oxidative stress in Parkinson\u2019s disease: An observational study of injury biomarkers. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146129"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1002\/tox.23543","article-title":"Iron-induced cellular in vitro neurotoxic responses in rat C6 cell line","volume":"37","author":"Urati","year":"2022","journal-title":"Environ. Toxicol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/0022-1910(89)90113-3","article-title":"Changes in haemolymph serotonin levels associated with feeding in the blood-sucking bug, Rhodnius prolixus","volume":"35","author":"Lange","year":"1989","journal-title":"J. Insect Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1186\/s13071-023-05957-0","article-title":"MicroRNA-989 targets 5-hydroxytryptamine receptor1 to regulate ovarian development and eggs production in Culex pipiens pallens","volume":"16","author":"Zheng","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Gao, L., Zhang, B., Feng, Y., Yang, W., Zhang, S., and Wang, J. (2024). Host 5-HT affects Plasmodium transmission in mosquitoes via modulating mosquito mitochondrial homeostasis. PLoS Pathog., 20.","DOI":"10.1101\/2024.02.19.580972"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/1753495X20932426","article-title":"Iron deficiency anaemia in pregnancy: A contemporary review","volume":"14","author":"Benson","year":"2021","journal-title":"Obstet. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Ferreira, A., Neves, P., and Gozzelino, R. (2019). Multilevel impacts of iron in the brain: The cross talk between neurophysiological mechanisms, cognition, and social behavior. Pharmaceuticals, 12.","DOI":"10.3390\/ph12030126"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"145810","DOI":"10.1016\/j.gene.2021.145810","article-title":"Hemocyte RNA-Seq analysis of Indian malarial vectors Anopheles stephensi and Anopheles culicifacies: From similarities to differences","volume":"798","author":"Rani","year":"2021","journal-title":"Gene"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/15\/1315\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:17:46Z","timestamp":1784787466000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/15\/15\/1315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":49,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["cells15151315"],"URL":"https:\/\/doi.org\/10.3390\/cells15151315","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]}}}