{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T09:23:15Z","timestamp":1787304195550,"version":"build-2736575974"},"reference-count":82,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 1631"],"award-info":[{"award-number":["GRK 1631"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC2049-390688087"],"award-info":[{"award-number":["EXC2049-390688087"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["400728090"],"award-info":[{"award-number":["400728090"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"award":["GRK 1631"],"award-info":[{"award-number":["GRK 1631"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EXC2049-390688087"],"award-info":[{"award-number":["EXC2049-390688087"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["400728090"],"award-info":[{"award-number":["400728090"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Muscle stem cells (MuSCs) are the cellular source for the generation and regeneration of skeletal muscle. Proper muscle growth requires precise control over the differentiation and self-renewal of MuSCs. Signaling systems, such as bone morphogenetic proteins (BMPs) and Notch, suppress the myogenic differentiation of MuSCs. This allows the expansion of the progenitor pool necessary for muscle growth. To better understand the molecular mechanisms and target genes of BMPs during myogenesis, we examined the response of adult mouse MuSCs to BMP6. BMP6 stimulation of freshly isolated MuSCs suppressed myogenic differentiation. Short-term stimulation (one hour) rapidly increased the expression of classical BMP target genes, such as Id1, as well as Notch pathway genes, including Hes1, Hey1, Lfng, and Snai1. We used Cleavage Under Targets and Tagmentation (CUT&amp;Tag) to generate whole-genome binding profiles for pSMAD1\/5\/9 and SMAD4, which are transcriptional effectors of the BMP pathway. This method detected dynamic binding in promoters and regulatory elements of direct BMP targets, including Notch pathway genes. Our data demonstrate that BMP6 is a potent suppressor of MuSC differentiation and reveal that a subset of well-characterized anti-myogenic genes (i.e., Hes1 and Hey1) are shared targets of the BMP and Notch pathways.<\/jats:p>","DOI":"10.3390\/cells15161490","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T09:30:53Z","timestamp":1787131853000},"page":"1490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stimulation of Adult Muscle Stem Cells with BMPs Results in Direct Activation of Notch Pathway Genes"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8538-7344","authenticated-orcid":false,"given":"Birthe Katrin Alexandra","family":"Lange","sequence":"first","affiliation":[{"name":"Department of Neuropediatrics, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"Department of Biology, Humboldt-Universit\u00e4t zu Berlin, Unter den Linden 6, 10099 Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioanna","family":"Polydorou","sequence":"additional","affiliation":[{"name":"Department of Neuropediatrics, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"},{"name":"U1179 Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines (UVSQ)\u2014Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), 78180 Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1691-4919","authenticated-orcid":false,"given":"Viktoriia","family":"Huryn","sequence":"additional","affiliation":[{"name":"Berlin Institute for Medical Systems Biology (BIMSB), Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), Hannoversche Str. 28, 10115 Berlin, Germany"},{"name":"Department of Computer Science, Humboldt-Universit\u00e4t zu Berlin, Unter den Linden 6, 10099 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3215-427X","authenticated-orcid":false,"given":"Angelina M.","family":"Georgieva","sequence":"additional","affiliation":[{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"You","sequence":"additional","affiliation":[{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"},{"name":"Integrative Biomedicine, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany"},{"name":"Department of Biology, Chemistry and Pharmacy, Freie Universit\u00e4t Berlin, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"},{"name":"Integrative Biomedicine, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-2092","authenticated-orcid":false,"given":"Susanne","family":"Morales-Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Neuropediatrics, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Brandt","sequence":"additional","affiliation":[{"name":"Integrative Biomedicine, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-8872","authenticated-orcid":false,"given":"Carmen","family":"Birchmeier","sequence":"additional","affiliation":[{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"},{"name":"Integrative Biomedicine, Max-Delbr\u00fcck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helge","family":"Amthor","sequence":"additional","affiliation":[{"name":"U1179 Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines (UVSQ)\u2014Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), 78180 Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2824-3891","authenticated-orcid":false,"given":"Markus","family":"Schuelke","sequence":"additional","affiliation":[{"name":"Department of Neuropediatrics, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charit\u00e9\u2014University Medical Center Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Charit\u00e9platz 1, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.4161\/cc.4.10.2114","article-title":"Self-Renewal of the Adult Skeletal Muscle Satellite Cell","volume":"4","author":"Collins","year":"2005","journal-title":"Cell Cycle"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.semcdb.2015.09.017","article-title":"PAX3 and PAX7 as Upstream Regulators of Myogenesis","volume":"44","author":"Buckingham","year":"2015","journal-title":"Semin. 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