{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:57:48Z","timestamp":1792789068017,"version":"4.5.0"},"reference-count":59,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Special Scientific Research Funds for Central Non-profit Institutes"},{"name":"Chinese Academy of Fishery Sciences","award":["2025A008"],"award-info":[{"award-number":["2025A008"]}],"id":[{"id":"https:\/\/ror.org\/02bwk9n38","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Chinese Academy of Fishery Sciences","award":["2023XT0102"],"award-info":[{"award-number":["2023XT0102"]}],"id":[{"id":"https:\/\/ror.org\/02bwk9n38","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2025A008"],"award-info":[{"award-number":["2025A008"]}],"id":[{"id":"https:\/\/ror.org\/02bwk9n38","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2023XT0102"],"award-info":[{"award-number":["2023XT0102"]}],"id":[{"id":"https:\/\/ror.org\/02bwk9n38","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["6242036"],"award-info":[{"award-number":["6242036"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Long-chain polyunsaturated fatty acids (LC-PUFAs), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are essential nutrients for human health, and fish and aquatic products are the primary dietary sources of these bioactive lipids. The endogenous capacity of teleosts to synthesize LC-PUFAs directly determines their nutritional value for human consumers. However, the genetic regulators governing this biosynthetic pathway remain largely unexplored. Here, we show that targeted disruption of dmrt2a, a transcription factor gene, in zebrafish (Danio rerio) via CRISPR\/Cas9, alters hepatic LC-PUFA composition and lipid homeostasis. Homozygous dmrt2a\u2212\/\u2212 mutants exhibited significantly reduced molar percentages of EPA (C20:5n-3) and DHA (C22:6n-3) in the liver, accompanied by the accumulation of their precursors, C18:2n-6 and C18:3n-3. In addition, the mutants developed a hepatic lipid metabolic disorder characterized by pathological accumulation of lipid droplets and elevated triglyceride levels, thereby providing a novel zebrafish model for research on hepatic steatosis. Transcriptomic profiling revealed that differentially expressed genes between dmrt2a\u2212\/\u2212 mutant and wild-type livers were significantly enriched in fatty acid biosynthesis and metabolic pathways. Notably, an LC-PUFA-enriched diet partially rescued the aberrant hepatic lipid phenotype. To our knowledge, this is the first study in any fish species to link a DMRT-family transcription factor to hepatic LC-PUFA composition and lipid homeostasis, suggesting dmrt2a as a potential target for genetic breeding to improve the nutritional quality of aquaculture products.<\/jats:p>","DOI":"10.3390\/cells15191763","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T08:18:46Z","timestamp":1790583526000},"page":"1763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loss of dmrt2a Alters Hepatic LC-PUFA Composition and Lipid Homeostasis in Zebrafish"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8103-4416","authenticated-orcid":false,"given":"Mingxi","family":"Hou","sequence":"first","affiliation":[{"name":"Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghao","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengde","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Genomics, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fishery Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.aquaculture.2015.01.010","article-title":"Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective","volume":"449","author":"Tocher","year":"2015","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Sun, J., Li, J., Li, Y., Du, J., Zhao, N., Mai, K., and Ai, Q. 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