{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:57:49Z","timestamp":1792789069616,"version":"4.5.0"},"reference-count":70,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union NextGeneration EU","award":["BG-RRP-2.004-0003"],"award-info":[{"award-number":["BG-RRP-2.004-0003"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Union NextGeneration EU","award":["BG16RFPR002-1.014-0002-C001"],"award-info":[{"award-number":["BG16RFPR002-1.014-0002-C001"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Medical University-Pleven, Bulgaria","award":["13\/2026"],"award-info":[{"award-number":["13\/2026"]}],"id":[{"id":"https:\/\/ror.org\/049ztct72","id-type":"ROR","asserted-by":"crossref"}]},{"award":["13\/2026"],"award-info":[{"award-number":["13\/2026"]}],"id":[{"id":"https:\/\/ror.org\/049ztct72","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Spanish Association Against Cancer","award":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"]}],"id":[{"id":"https:\/\/ror.org\/00gxct719","id-type":"ROR","asserted-by":"crossref"}]},{"award":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"]}],"id":[{"id":"https:\/\/ror.org\/00gxct719","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Consorcio Centro de Investigaci\u00f3n Biom\u00e9dica en Red","award":["CB06\/01\/1026"],"award-info":[{"award-number":["CB06\/01\/1026"]}],"id":[{"id":"https:\/\/ror.org\/02g87qh62","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The tumor microenvironment (TME) is shaped by dynamic interactions between malignant cells and stromal components, with mesenchymal stem cells (MSCs) contributing to extracellular matrix (ECM) remodeling. Here, we examined how secretomes from two hematological cancer cell lines, RPMI-8226 (multiple myeloma) and HG-3 (B-cell leukemia), influence early functional responses of human adipose-derived MSCs (AD-MSCs) and their interaction with and reorganization of collagen. Cancer-conditioned media accelerated MSC proliferation (~2-fold faster doubling time) and increased SA-\u03b2-galactosidase activity (~3\u20134-fold above control). Upon adhesion to collagen, treated MSCs displayed distinct morphologies and reduced cell spreading (~2100 \u03bcm2 vs. ~2500 \u03bcm2 in controls), accompanied by altered collagen organization. To quantify collagen remodeling, we introduced the collagen removal index (CRI), expressing reorganized collagen area relative to cell spreading area. CRI revealed higher remodeling efficiency in HG-3-treated MSCs than in RPMI-8226-treated and control cells, indicating enhanced collagen-interacting capacity. Secretome exposure also modulated cytokine secretion, with increased bFGF under both conditions and IL-6 upregulation specifically following HG-3 exposure. After secretome withdrawal, most functional alterations, including senescence-associated changes, motility, and elevated bFGF, persisted. Notably, IL-6 upregulation and increased CRI emerged in RPMI-8226-exposed MSCs after withdrawal, suggesting a delayed response. Together, these findings reveal early, partially persistent functional shifts in MSC behavior and collagen remodeling that may represent an early stromal response with potential relevance to tumor progression.<\/jats:p>","DOI":"10.3390\/cells15191775","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:14:37Z","timestamp":1790694877000},"page":"1775","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Paracrine Signals from the Tumor Microenvironment Modulate Stem Cell Collagen Interaction and Remodeling, Indicating Early Functional Changes"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7279-4348","authenticated-orcid":false,"given":"Lora","family":"Topalova","sequence":"first","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"},{"name":"Center of Competence in Personalized Medicine, 3D and Telemedicine, Robotic Assisted and Minimally Invasive Surgery\u2014\u201cLeonardo da Vinci\u201d, 5800 Pleven, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6880-4174","authenticated-orcid":false,"given":"Tanya","family":"Stoyanova","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"},{"name":"Center of Competence in Personalized Medicine, 3D and Telemedicine, Robotic Assisted and Minimally Invasive Surgery\u2014\u201cLeonardo da Vinci\u201d, 5800 Pleven, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7530-207X","authenticated-orcid":false,"given":"Nadia","family":"Garc\u00eda-Parra","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, Polytechnic University of Valencia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9099-0885","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"G\u00f3mez Ribelles","sequence":"additional","affiliation":[{"name":"Centre for Biomaterials and Tissue Engineering, Polytechnic University of Valencia, 46022 Valencia, Spain"},{"name":"Biomedical Research Center Networking in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7108-051X","authenticated-orcid":false,"given":"Svetla","family":"Todinova","sequence":"additional","affiliation":[{"name":"Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Altankov","sequence":"additional","affiliation":[{"name":"Center of Competence in Personalized Medicine, 3D and Telemedicine, Robotic Assisted and Minimally Invasive Surgery\u2014\u201cLeonardo da Vinci\u201d, 5800 Pleven, Bulgaria"},{"name":"Associate Project BG-RRP-2.004-0003, Medical University Pleven, 5800 Pleven, Bulgaria"},{"name":"Research Institute, Medical University Pleven, 5800 Pleven, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1002\/sctm.17-0051","article-title":"Mesenchymal Stem Cells: Time to Change the Name!","volume":"6","author":"Caplan","year":"2017","journal-title":"Stem Cells Transl. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage Potential of Adult Human Mesenchymal Stem Cells","volume":"284","author":"Pittenger","year":"1999","journal-title":"Science"},{"key":"ref_3","first-page":"3603","article-title":"Bone Marrow-Derived Mesenchymal Stem Cells as Vehicles for Interferon-Beta Delivery into Tumors","volume":"62","author":"Studeny","year":"2002","journal-title":"Cancer Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Jin, J., Lin, J., Xu, A., Lou, J., Qian, C., Li, X., Wang, Y., Yu, W., and Tao, H. (2021). CCL2: An Important Mediator Between Tumor Cells and Host Cells in Tumor Microenvironment. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.722916"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1002\/cam4.4521","article-title":"The Role of Mesenchymal Stem Cells in the Occurrence, Development, and Therapy of Hepatocellular Carcinoma","volume":"11","author":"Zhang","year":"2022","journal-title":"Cancer Med."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Spaeth, E.L., Dembinski, J.L., Sasser, A.K., Watson, K., Klopp, A., Hall, B., Andreeff, M., and Marini, F. (2009). Mesenchymal Stem Cell Transition to Tumor-Associated Fibroblasts Contributes to Fibrovascular Network Expansion and Tumor Progression. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0004992"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/stem.559","article-title":"Concise Review: Dissecting a Discrepancy in the Literature: Do Mesenchymal Stem Cells Support or Suppress Tumor Growth?","volume":"29","author":"Klopp","year":"2010","journal-title":"Stem Cells"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/nrc.2016.73","article-title":"The Biology and Function of Fibroblasts in Cancer","volume":"16","author":"Kalluri","year":"2016","journal-title":"Nat. Rev. Cancer"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108473","DOI":"10.1016\/j.celrep.2020.108473","article-title":"Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis","volume":"33","author":"Fan","year":"2020","journal-title":"Cell Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/s41419-023-06110-6","article-title":"Stromal Cells in the Tumor Microenvironment: Accomplices of Tumor Progression?","volume":"14","author":"Zhao","year":"2023","journal-title":"Cell Death Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1038\/s12276-023-01013-0","article-title":"Cancer-Associated Fibroblasts: From Basic Science to Anticancer Therapy","volume":"55","author":"Yang","year":"2023","journal-title":"Exp. Mol. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6916","DOI":"10.18632\/oncotarget.6870","article-title":"Human Carcinoma-Associated Mesenchymal Stem Cells Promote Ovarian Cancer Chemotherapy Resistance via a BMP4\/HH Signaling Loop","volume":"7","author":"Coffman","year":"2016","journal-title":"Oncotarget"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/stem.2932","article-title":"Ovarian Carcinoma-Associated Mesenchymal Stem Cells Arise from Tissue-Specific Normal Stroma","volume":"37","author":"Coffman","year":"2019","journal-title":"Stem Cells"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s40164-025-00688-7","article-title":"Tumor-Associated Mesenchymal Stem\/Stromal Cells in Tumor Microenvironment and Carcinogenesis","volume":"14","author":"Sun","year":"2025","journal-title":"Exp. Hematol. Oncol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1016\/j.jprot.2010.04.003","article-title":"The Cancer Cell Secretome: A Good Source for Discovering Biomarkers?","volume":"73","author":"Pavlou","year":"2010","journal-title":"J. Proteom."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/gt.2008.39","article-title":"Inflammation and Tumor Microenvironments: Defining the Migratory Itinerary of Mesenchymal Stem Cells","volume":"15","author":"Spaeth","year":"2008","journal-title":"Gene Ther."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s12943-017-0597-8","article-title":"Mesenchymal Stem Cells: Key Players in Cancer Progression","volume":"16","author":"Ridge","year":"2017","journal-title":"Mol. Cancer"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Maiso, P., Mogoll\u00f3n, P., Ocio, E.M., and Garayoa, M. (2021). Bone Marrow Mesenchymal Stromal Cells in Multiple Myeloma: Their Role as Active Contributors to Myeloma Progression. Cancers, 13.","DOI":"10.3390\/cancers13112542"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.ccell.2016.09.011","article-title":"Characteristics and Significance of the Pre-Metastatic Niche","volume":"30","author":"Liu","year":"2016","journal-title":"Cancer Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1146\/annurev-pathol-121808-102144","article-title":"The Senescence-Associated Secretory Phenotype: The Dark Side of Tumor Suppression","volume":"5","author":"Desprez","year":"2010","journal-title":"Annu. Rev. Pathol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Turinetto, V., Vitale, E., and Giachino, C. (2016). Senescence in Human Mesenchymal Stem Cells: Functional Changes and Implications in Stem Cell-Based Therapy. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071164"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s10555-022-10069-9","article-title":"Mesenchymal Stromal Cell Senescence in Haematological Malignancies","volume":"42","author":"Plakhova","year":"2023","journal-title":"Cancer Metastasis Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1038\/s41568-026-00936-w","article-title":"Mesenchymal Stromal\/Stem Cells in Tumour Initiation, Progression and Therapy","volume":"26","author":"Garcia","year":"2026","journal-title":"Nat. Rev. Cancer"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Basisty, N., Kale, A., Jeon, O.H., Kuehnemann, C., Payne, T., Rao, C., Holtz, A., Shah, S., Sharma, V., and Ferrucci, L. (2020). A Proteomic Atlas of Senescence-Associated Secretomes for Aging Biomarker Development. PLoS Biol., 18.","DOI":"10.1371\/journal.pbio.3000599"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.tcb.2018.02.001","article-title":"Hallmarks of Cellular Senescence","volume":"28","author":"Nehme","year":"2018","journal-title":"Trends Cell Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41572-024-00529-7","article-title":"Multiple Myeloma","volume":"10","author":"Malard","year":"2024","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1200\/JCO.18.02096","article-title":"Treatment of Multiple Myeloma: ASCO and CCO Joint Clinical Practice Guideline","volume":"37","author":"Mikhael","year":"2019","journal-title":"J. Clin. Oncol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.3324\/haematol.2020.268037","article-title":"Targeting the Tumor Microenvironment in Chronic Lymphocytic Leukemia","volume":"106","author":"Svanberg","year":"2021","journal-title":"Haematologica"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"322","DOI":"10.4252\/wjsc.v6.i3.322","article-title":"Multiple Myeloma Mesenchymal Stromal Cells: Contribution to Myeloma Bone Disease and Therapeutics","volume":"6","author":"Miguel","year":"2014","journal-title":"World J. Stem Cells"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1172\/JCI66517","article-title":"BM Mesenchymal Stromal Cell-Derived Exosomes Facilitate Multiple Myeloma Progression","volume":"123","author":"Roccaro","year":"2013","journal-title":"J. Clin. Investig."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1126\/science.1164390","article-title":"Leukemic Cells Create Bone Marrow Niches That Disrupt the Behavior of Normal Hematopoietic Progenitor Cells","volume":"322","author":"Colmone","year":"2008","journal-title":"Science"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1182\/blood-2014-12-618025","article-title":"Exosomes Released by Chronic Lymphocytic Leukemia Cells Induce the Transition of Stromal Cells into Cancer-Associated Fibroblasts","volume":"126","author":"Paggetti","year":"2015","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Dander, E., Palmi, C., D\u2019amico, G., and Cazzaniga, G. (2021). The Bone Marrow Niche in B-Cell Acute Lymphoblastic Leukemia: The Role of Microenvironment from Pre-Leukemia to Overt Leukemia. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094426"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e201900304","DOI":"10.26508\/lsa.201900304","article-title":"Mesenchymal Stromal Cell Activation by Breast Cancer Secretomes in Bioengineered 3D Microenvironments","volume":"2","author":"Blache","year":"2019","journal-title":"Life Sci. Alliance"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4","DOI":"10.12703\/r\/10-4","article-title":"The Cancer Cell Secretome Drives Cooperative Manipulation of the Tumour Microenvironment to Accelerate Tumourigenesis","volume":"10","author":"Ritchie","year":"2021","journal-title":"Fac. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Fairfield, H., Costa, S., Falank, C., Farrell, M., Murphy, C.S., D\u2019Amico, A., Driscoll, H., and Reagan, M.R. (2021). Multiple Myeloma Cells Alter Adipogenesis, Increase Senescence-Related and Inflammatory Gene Transcript Expression, and Alter Metabolism in Preadipocytes. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.584683"},{"key":"ref_37","first-page":"9012152","article-title":"Multiple Myeloma-Derived Exosomes Regulate the Functions of Mesenchymal Stem Cells Partially via Modulating MiR-21 and MiR-146a","volume":"2017","author":"Liu","year":"2017","journal-title":"Stem Cells Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.canlet.2016.04.024","article-title":"Induction of MiR-146a by Multiple Myeloma Cells in Mesenchymal Stromal Cells Stimulates Their pro-Tumoral Activity","volume":"377","author":"Wang","year":"2016","journal-title":"Cancer Lett."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1111\/j.1365-2141.2012.09082.x","article-title":"In Vitro Anti-Myeloma Activity of TRAIL-Expressing Adipose-Derived Mesenchymal Stem Cells","volume":"157","author":"Ciavarella","year":"2012","journal-title":"Br. J. Haematol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"B\u00e9r\u00e9ziat, V., Mazurier, C., Auclair, M., Ferrand, N., Jolly, S., Marie, T., Kobari, L., Toillon, I., Delhommeau, F., and F\u00e8ve, B. (2019). Systemic Dysfunction of Osteoblast Differentiation in Adipose-Derived Stem Cells from Patients with Multiple Myeloma. Cells, 8.","DOI":"10.3390\/cells8050441"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1158\/1541-7786.MCR-09-0015","article-title":"CXCR4 and MMP-1 Are Elevated in Breast Carcinoma-Associated Fibroblasts and in Normal Mammary Fibroblasts Exposed to Factors Secreted by Breast Cancer Cells","volume":"7","author":"Eck","year":"2009","journal-title":"Mol. Cancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Basmaeil, Y., Al Subayyil, A., Abumaree, M., and Khatlani, T. (2021). Conditions Mimicking the Cancer Microenvironment Modulate the Functional Outcome of Human Chorionic Villus Mesenchymal Stem\/Stromal Cells in Vitro. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.650125"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1182\/bloodadvances.2023011632","article-title":"Stromal Alterations in Patients with Monoclonal Gammopathy of Undetermined Significance, Smoldering Myeloma, and Multiple Myeloma","volume":"8","author":"Bogun","year":"2024","journal-title":"Blood Adv."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Stirling, D.R., Swain-Bowden, M.J., Lucas, A.M., Carpenter, A.E., Cimini, B.A., and Goodman, A. (2021). CellProfiler 4: Improvements in Speed, Utility and Usability. BMC Bioinform., 22.","DOI":"10.1186\/s12859-021-04344-9"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1038\/nprot.2015.016","article-title":"Cell Cycle Staging of Individual Cells by Fluorescence Microscopy","volume":"10","author":"Roukos","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ligasov\u00e1, A., Frydrych, I., and Koberna, K. (2023). Basic Methods of Cell Cycle Analysis. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24043674"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.chroma.2007.01.020","article-title":"Study of Posttranslational Non-Enzymatic Modifications of Collagen Using Capillary Electrophoresis\/Mass Spectrometry and High Performance Liquid Chromatography\/Mass Spectrometry","volume":"1155","author":"Eckhardt","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e2919","DOI":"10.21769\/BioProtoc.2919","article-title":"Fluorescent Labeling of Rat-Tail Collagen for 3D Fluorescence Imaging","volume":"8","author":"Doyle","year":"2018","journal-title":"Bio-Protocol"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Suarez-Arnedo, A., Figueroa, F.T., Clavijo, C., Arbel\u00e1ez, P., Cruz, J.C., and Mu\u00f1oz-Camargo, C. (2020). An Image J Plugin for the High Throughput Image Analysis of in Vitro Scratch Wound Healing Assays. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232565"},{"key":"ref_50","first-page":"1276","article-title":"The Molecular Probes Handbook. A Guide to Fluorescent Probes and Labeling Technologies","volume":"76","author":"Wiederschain","year":"2011","journal-title":"Biochemistry"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1182\/blood-2012-01-379024","article-title":"Myeloma as a Model for the Process of Metastasis: Implications for Therapy","volume":"120","author":"Ghobrial","year":"2012","journal-title":"Blood"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3367","DOI":"10.1182\/blood-2009-06-225326","article-title":"The Microenvironment in Mature B-Cell Malignancies: A Target for New Treatment Strategies","volume":"114","author":"Burger","year":"2009","journal-title":"Blood"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"34090","DOI":"10.18632\/oncotarget.26124","article-title":"Complex Formation between Platelet-Derived Growth Factor Receptor \u03b2 and Transforming Growth Factor \u03b2 Receptor Regulates the Differentiation of Mesenchymal Stem Cells into Cancer-Associated Fibroblasts","volume":"9","author":"Aoto","year":"2018","journal-title":"Oncotarget"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4161\/cbt.26607","article-title":"Role of Chemokines and Their Receptors in Chronic Lymphocytic Leukemia: Function in Microenvironment and Targeted Therapy","volume":"15","author":"Han","year":"2014","journal-title":"Cancer Biol. Ther."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1038\/s41568-019-0238-1","article-title":"A Framework for Advancing Our Understanding of Cancer-Associated Fibroblasts","volume":"20","author":"Sahai","year":"2020","journal-title":"Nat. Rev. Cancer"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4252\/wjsc.v15.i5.421","article-title":"Communication between Bone Marrow Mesenchymal Stem Cells and Multiple Myeloma Cells: Impact on Disease Progression","volume":"15","year":"2023","journal-title":"World J. Stem Cells"},{"key":"ref_57","first-page":"983","article-title":"Myeloma Bone Disease: Pathogenesis and Treatment","volume":"19","author":"Roodman","year":"2005","journal-title":"Oncology"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gagliano, T., and Brancolini, C. (2021). Epigenetic Mechanisms beyond Tumour\u2013Stroma Crosstalk. Cancers, 13.","DOI":"10.3390\/cancers13040914"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.2217\/epi-2016-0110","article-title":"Epigenetic Control of the Tumor Microenvironment","volume":"8","author":"Marks","year":"2016","journal-title":"Epigenomics"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"307","DOI":"10.3109\/15419069809040300","article-title":"Adhesive Interactions of Human Multiple Myeloma Cell Lines with Different Extracellular Matrix Molecules","volume":"5","author":"Kibler","year":"1998","journal-title":"Cell Adhes. Commun."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Dubois, N., Crompot, E., Meuleman, N., Bron, D., Lagneaux, L., and Stamatopoulos, B. (2020). Importance of Crosstalk Between Chronic Lymphocytic Leukemia Cells and the Stromal Microenvironment: Direct Contact, Soluble Factors, and Extracellular Vesicles. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.01422"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s10238-025-01908-4","article-title":"The Emerging Role of Cancer-Associated Fibroblasts in Hematological Malignancies: From Their Influence on Tumor Progression and Drug Resistance to Novel Therapeutic Opportunities","volume":"25","author":"Jalilivand","year":"2025","journal-title":"Clin. Exp. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1053\/j.seminhematol.2023.12.004","article-title":"Role of the Tumor Microenvironment in CLL Pathogenesis","volume":"61","author":"Hallek","year":"2024","journal-title":"Semin. Hematol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.1182\/blood-2002-09-2907","article-title":"Paracrine Interactions of Basic Fibroblast Growth Factor and Interleukin-6 in Multiple Myeloma","volume":"101","author":"Bisping","year":"2003","journal-title":"Blood"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1093\/stmcls\/sxac036","article-title":"Carcinoma-Associated Mesenchymal Stem\/Stromal Cells: Architects of the Pro-Tumorigenic Tumor Microenvironment","volume":"40","author":"Frisbie","year":"2022","journal-title":"Stem Cells"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Sch\u00fctt, J., N\u00e4gler, T., Schenk, T., and Brioli, A. (2021). Investigating the Interplay between Myeloma Cells and Bone Marrow Stromal Cells in the Development of Drug Resistance: Dissecting the Role of Epigenetic Modifications. Cancers, 13.","DOI":"10.3390\/cancers13164069"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.stem.2016.06.001","article-title":"Leukemic Stem Cells Evade Chemotherapy by Metabolic Adaptation to an Adipose Tissue Niche","volume":"19","author":"Ye","year":"2016","journal-title":"Cell Stem Cell"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Sheng, X., and Mittelman, S.D. (2014). The Role of Adipose Tissue and Obesity in Causing Treatment Resistance of Acute Lymphoblastic Leukemia. Front. Pediatr., 2.","DOI":"10.3389\/fped.2014.00053"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.leukres.2012.12.013","article-title":"Adipose Tissue Attracts and Protects Acute Lymphoblastic Leukemia Cells from Chemotherapy","volume":"37","author":"Pramanik","year":"2013","journal-title":"Leuk. Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1007\/s13679-025-00644-w","article-title":"Unraveling Obesity and Multiple Myeloma: Insights from Epidemiology and Molecular Mechanisms","volume":"14","author":"Manna","year":"2025","journal-title":"Curr. Obes. 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