{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:00:23Z","timestamp":1792789223018,"version":"4.5.0"},"reference-count":27,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["22K06589"],"award-info":[{"award-number":["22K06589"]}]},{"name":"President\u2019s Discretionary Fund of Niigata University of Pharmacy and Medical and Life Sciences","id":[{"id":"https:\/\/ror.org\/00dnbtf70","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/00dnbtf70","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>CCDC69 was previously identified as a gene upregulated during adipogenic differentiation of human Simpson\u2013Golabi\u2013Behmel syndrome (SGBS) preadipocytes but not mouse 3T3-L1 preadipocytes. Here, the role of CCDC69 in adipogenesis was investigated using SGBS cells. CCDC69 expression during adipogenic differentiation was assessed by quantitative reverse transcription PCR and Western blotting. CCDC69 function was examined by adenovirus-mediated overexpression and knockdown, followed by analyses of lipid accumulation, adipocyte marker expression, cell morphology, and cytoskeletal organization. CCDC69 expression increased during adipogenesis. CCDC69 overexpression enhanced adipogenesis, as indicated by increased lipid accumulation, a higher proportion of cells containing lipid droplets, and increased expression of adipocyte marker genes and proteins. Unexpectedly, CCDC69 knockdown also enhanced adipogenesis. Time-course analysis showed that CCDC69 overexpression suppressed PPARG mRNA expression during early adipogenesis but increased its expression at later stages, whereas knockdown increased PPARG expression at later stages. CCDC69 overexpression induced a flattened cell morphology accompanied by reduced fibrillar \u03b1-tubulin structures and F-actin stress fibers, whereas knockdown increased the number of small, round cells. These findings suggest that the effects of CCDC69 on adipogenesis may vary across the differentiation process in SGBS cells and are associated with adipocyte morphology and cytoskeletal organization.<\/jats:p>","DOI":"10.3390\/cells15191782","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:25:42Z","timestamp":1790760342000},"page":"1782","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CCDC69 Regulates Adipocyte Differentiation and Cellular Morphology in SGBS Cells\u2014A Human Preadipocyte Cell Line"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1280-900X","authenticated-orcid":false,"given":"Takeo","family":"Iwata","sequence":"first","affiliation":[{"name":"Department of Functional Morphology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Medical and Life Sciences, 265-1 Higashijima, Akiha-ku, Niigata City 956-8603, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"ref_1","first-page":"e984","article-title":"Obesity and Cardiovascular Disease: A Scientific Statement from the American Heart Association","volume":"143","author":"Poirier","year":"2021","journal-title":"Circulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1172\/JCI45887","article-title":"Adipose Tissue Remodeling and Obesity","volume":"121","author":"Sun","year":"2011","journal-title":"J. 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