{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:34:51Z","timestamp":1790951691336,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T00:00:00Z","timestamp":1551312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong","doi-asserted-by":"publisher","award":["ZR2016CM18"],"award-info":[{"award-number":["ZR2016CM18"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Undergraduate Training Programs for Innovation and Entrepreneurship","award":["201710433207"],"award-info":[{"award-number":["201710433207"]}]},{"name":"Key Technology Innovation Project of Shandong Province","award":["2018CXGC0102"],"award-info":[{"award-number":["2018CXGC0102"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>In this study, the mechanism by which GSK-3\u03b2 regulates protein synthesis and lipid deposition was investigated in zebrafish (Danio rerio). The vector of pEGFP-N1-GSK-3\u03b2 was constructed and injected into the muscle of zebrafish. It was found that the mRNA and protein expression of tuberous sclerosis complex 2 (TSC2) was significantly increased. However, the mRNA and protein expression of mammalian target of rapamycin (mTOR), p70 ribosomal S6 kinase 1 (S6K1), and 4E-binding protein 1 (4EBP1) was significantly decreased by the pEGFP-N1-GSK-3\u03b2 vector in the muscle of zebrafish. In addition, the mRNA and protein expression of \u03b2-catenin, CCAAT\/enhancer binding protein \u03b1 (C\/EBP\u03b1), and peroxisome proliferators-activated receptor \u03b3 (PPAR\u03b3) was significantly decreased, but the mRNA expression of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), ATP-citrate lyase (ACL), and HMG-CoA reductase (HMGCR) was significantly increased by the pEGFP-N1-GSK-3\u03b2 vector. The activity of FAS, ACC, ACL, and HMGCR as well as the content of triglyceride (TG), total cholesterol (TC), and nonesterified fatty acids (NEFA) were significantly increased by the pEGFP-N1-GSK-3\u03b2 vector in the muscle of zebrafish. The content of free amino acids Arg, Lys, His, Phe, Leu, Ile, Val, and Thr was significantly decreased by the pEGFP-N1-GSK-3\u03b2 vector. The results indicate that GSK-3\u03b2 may participate in regulating protein synthesis via TSC2\/mTOR signaling and regulating lipid deposition via \u03b2-catenin in the muscle of zebrafish (Danio rerio).<\/jats:p>","DOI":"10.3390\/cells8030205","type":"journal-article","created":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T03:29:21Z","timestamp":1551410961000},"page":"205","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["GSK-3\u03b2 at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish"],"prefix":"10.3390","volume":"8","author":[{"given":"Yaqi","family":"Gu","sequence":"first","affiliation":[{"name":"Anti-aging &amp; Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Gao","sequence":"additional","affiliation":[{"name":"Anti-aging &amp; Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Han","sequence":"additional","affiliation":[{"name":"Sunwei Biotech Shandong Co., Ltd., Weifang 261205, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Li","sequence":"additional","affiliation":[{"name":"Anti-aging &amp; Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairui","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Biological and Agricultural Engineering, Weifang Bioengineering Technology Research Center, Weifang University, Weifang 261061, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongwu","family":"Liu","sequence":"additional","affiliation":[{"name":"Anti-aging &amp; Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo 255000, China"},{"name":"School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuxiang","family":"Pang","sequence":"additional","affiliation":[{"name":"Anti-aging &amp; Regenerative Medicine Research Institution, School of Life Sciences, Shandong University of Technology, Zibo 255000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1242\/jcs.00384","article-title":"GSK-3: Tricks of the trade for a multitasking kinase","volume":"116","author":"Doble","year":"2003","journal-title":"J. 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