{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:47:21Z","timestamp":1790621241585,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T00:00:00Z","timestamp":1563840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["13\/25950-1 and 10\/19410-9"],"award-info":[{"award-number":["13\/25950-1 and 10\/19410-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the &lt;200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the &lt;200- and &gt;200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related to protein modification, translation, and DNA metabolism\/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription\/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA content.<\/jats:p>","DOI":"10.3390\/cells8070765","type":"journal-article","created":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T10:44:51Z","timestamp":1563878691000},"page":"765","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9641-1792","authenticated-orcid":false,"given":"Roberta","family":"Peres da Silva","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de S\u00e3o Paulo (EPM-UNIFESP), S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa G. V.","family":"Longo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de S\u00e3o Paulo (EPM-UNIFESP), S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia P. C. da","family":"Cunha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Ciclo Celular\u2014Center of Toxins, Immune Response and Cell Signaling-Center (CeTICS), Butantan Institute, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago J. P.","family":"Sobreira","sequence":"additional","affiliation":[{"name":"Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6081-3439","authenticated-orcid":false,"given":"Marcio L.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas\u2014FIOCRUZ PR, Curitiba 81350-010, Brazil"},{"name":"Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helisson","family":"Faoro","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas\u2014FIOCRUZ PR, Curitiba 81350-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4892-163X","authenticated-orcid":false,"given":"Samuel","family":"Goldenberg","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas\u2014FIOCRUZ PR, Curitiba 81350-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1972-2658","authenticated-orcid":false,"given":"Lysangela R.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas\u2014FIOCRUZ PR, Curitiba 81350-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3332-0487","authenticated-orcid":false,"given":"Rosana","family":"Puccia","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina-Universidade Federal de S\u00e3o Paulo (EPM-UNIFESP), S\u00e3o Paulo 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Martinez, R. 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