{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:38:52Z","timestamp":1779892732917,"version":"3.53.1"},"reference-count":49,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T00:00:00Z","timestamp":1572393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1160777"],"award-info":[{"award-number":["1160777"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["3180621"],"award-info":[{"award-number":["3180621"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Glioblastoma is the brain tumor with the worst prognosis. This is mainly due to a cell subpopulation with an extremely aggressive potential, called glioblastoma stem-like cells (GSCs). These cells produce high levels of extracellular adenosine, which are increased even more under hypoxic conditions. Under hypoxia, adenosine signaling is related to HIF-2\u03b1 expression, enhancing cell aggressiveness. Adenosine can be degraded using recombinant adenosine deaminase (ADA) to revert its pathological effects. The aim of this study was to degrade adenosine using ADA in order to decrease malignancy of GSCs. Adenosine depletion was performed using recombinant ADA. Migration and invasion were measured by transwell and matrigel-coated transwell assay, respectively. HIF-2\u03b1-dependent cell migration\/invasion decreased in GSCs treated with ADA under hypoxia. MRPs-mediated chemoresistance and colony formation decreased in treatment with ADA. In conclusion, adenosine depletion using adenosine deaminase decreases GSCs aggressiveness.<\/jats:p>","DOI":"10.3390\/cells8111353","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T05:18:26Z","timestamp":1572499106000},"page":"1353","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Adenosine Depletion as A New Strategy to Decrease Glioblastoma Stem-Like Cells Aggressiveness"],"prefix":"10.3390","volume":"8","author":[{"given":"Ignacio","family":"Niechi","sequence":"first","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atenea","family":"Uribe-Ojeda","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ignacio","family":"Erices","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngelo","family":"Torres","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Uribe","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Dellis","family":"Rocha","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1353-6280","authenticated-orcid":false,"given":"Pamela","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans G.","family":"Richter","sequence":"additional","affiliation":[{"name":"Instituto de Anatom\u00eda, Histolog\u00eda y Patolog\u00eda, Facultad de Medicina, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rody","family":"San Mart\u00edn","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Quezada","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica y Microbiolog\u00eda, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 511-0566, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.canlet.2012.12.024","article-title":"Understanding high grade glioma: Molecular mechanism, therapy and comprehensive management","volume":"331","author":"Wang","year":"2013","journal-title":"Cancer Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1001\/jama.2013.280319","article-title":"Glioblastoma and Other Malignant Gliomas","volume":"310","author":"Omuro","year":"2013","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wen, P., and Kesari, S. 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