{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:03:34Z","timestamp":1791558214856,"version":"4.3.4"},"reference-count":53,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2019,11,17]],"date-time":"2019-11-17T00:00:00Z","timestamp":1573948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/23652-0 and 2019\/01109-2"],"award-info":[{"award-number":["2012\/23652-0 and 2019\/01109-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The lethal-7 (let-7) family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i and to assess their value as prognostic markers in uterine leiomyosarcoma (LMS) patients. The miRNAs expression profile was assessed in 34 LMS and 13 normal myometrium (MM) paraffin-embedded samples. All let-7 family members showed downregulation in LMS. Our findings showed that patients with let-7e downregulation had worse overall survival (OS) and is an independent prognostic factor (hazard ratio [HR] = 2.24). In addition, almost half the patients had distant metastasis. LMS patients with downregulated let-7b and let-7d had worse disease-free survival (DFS); they are not independent prognostic factors (HR = 2.65). Patients\u2019 ages were associated with let-7d, let-7e and let-7f (p = 0.0160) downregulation. In conclusion, all the let-7 family members were downregulated in LMS patients, and the greater the loss of expression of these molecules, the greater their relationship with worse prognosis of patients. Let-7e expression might influence the OS, while let-7b and le-7d might influence the DFS. The lowest expression levels of let-7d, let-7e, and let-7f were associated with the oldest patients. Our findings indicate strong evidence of let-7\u2019s role as a potential prognostic biomarker in LMS.<\/jats:p>","DOI":"10.3390\/cells8111452","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T04:31:10Z","timestamp":1574051470000},"page":"1452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Let-7 miRNA\u2019s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1641-5609","authenticated-orcid":false,"given":"Bruna Cristine","family":"de Almeida","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9027-8281","authenticated-orcid":false,"given":"Laura Gonzalez","family":"dos Anjos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyuki","family":"Uno","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (LIM 24), Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo (CTO\/ICESP) Av Dr Arnaldo 251 sala 23 8 andar, S\u00e3o Paulo 01246000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela Werneck","family":"da Cunha","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rede D\u2019OR-S\u00e3o Luiz, Rua das Perobas, 344-Jabaquara, S\u00e3o Paulo 04321-120, Brazil"},{"name":"Hospital A C Camargo Cancer Center, SP, BR R. Tamandar\u00e9, 753 Liberdade, S\u00e3o Paulo 05403-010, Brazil"},{"name":"National Institute for Science and Technology in Oncogenomics and Therapeutic Innovation, SP, BR R. Tamandar\u00e9, 753 Liberdade, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Augusto","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rede D\u2019OR-S\u00e3o Luiz, Rua das Perobas, 344-Jabaquara, S\u00e3o Paulo 04321-120, Brazil"},{"name":"Hospital A C Camargo Cancer Center, SP, BR R. Tamandar\u00e9, 753 Liberdade, S\u00e3o Paulo 05403-010, Brazil"},{"name":"National Institute for Science and Technology in Oncogenomics and Therapeutic Innovation, SP, BR R. Tamandar\u00e9, 753 Liberdade, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glauco","family":"Baiocchi","sequence":"additional","affiliation":[{"name":"Department of Gynecology Oncology, A.C.Camargo Cancer Center, Rua Prof Antonio Prudente 211, S\u00e3o Paulo 01509-001, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmund Chada","family":"Baracat","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia Candido","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetricia e Ginecologia, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, HCFMUSP, SP, BR Av. Dr Arnaldo 455, sala 4121, Cerqueira Cesar, S\u00e3o Paulo 05403-010, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, B., Jiang, L., and Xu, Q. (2018). A comprehensive evaluation for polymorphisms in let-7 family in cancer risk and prognosis: A system review and meta-analysis. Biosci. 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