{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:51:35Z","timestamp":1760241095012,"version":"build-2065373602"},"reference-count":63,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T00:00:00Z","timestamp":1574726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003451","name":"Euskal Herriko Unibertsitatea","doi-asserted-by":"publisher","award":["PPGA19\/08","EHUA15\/08"],"award-info":[{"award-number":["PPGA19\/08","EHUA15\/08"]}],"id":[{"id":"10.13039\/501100003451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003086","name":"Eusko Jaurlaritza","doi-asserted-by":"publisher","award":["Elkartek19\/72","IT599-13"],"award-info":[{"award-number":["Elkartek19\/72","IT599-13"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["BFU2015-66806-R"],"award-info":[{"award-number":["BFU2015-66806-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["RTI2018-094263-B-100"],"award-info":[{"award-number":["RTI2018-094263-B-100"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The transcription factor BrlA plays a central role in the production of asexual spores (conidia) in the fungus Aspergillus nidulans. BrlA levels are controlled by signal transducers known collectively as UDAs. Furthermore, it governs the expression of CDP regulators, which control most of the morphological transitions leading to the production of conidia. In response to the emergence of fungal cells in the air, the main stimulus triggering conidiation, UDA mutants such as the flbB deletant fail to induce brlA expression. Nevertheless, \u0394flbB colonies conidiate profusely when they are cultured on a medium containing high H2PO4\u2212 concentrations, suggesting that the need for FlbB activity is bypassed. We used this phenotypic trait and an UV-mutagenesis procedure to isolate \u0394flbB mutants unable to conidiate under these stress conditions. Transformation of mutant FLIP166 with a wild-type genomic library led to the identification of the putative transcription factor SocA as a multicopy suppressor of the FLIP (Fluffy, aconidial, In Phosphate) phenotype. Deregulation of socA altered both growth and developmental patterns. Sequencing of the FLIP166 genome enabled the identification and characterization of PmtCP282L as the recessive mutant form responsible for the FLIP phenotype. Overall, results validate this strategy for identifying genes\/mutations related to the control of conidiation.<\/jats:p>","DOI":"10.3390\/cells8121520","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T10:57:27Z","timestamp":1574765847000},"page":"1520","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identification and Characterization of Aspergillus nidulans Mutants Impaired in Asexual Development under Phosphate Stress"],"prefix":"10.3390","volume":"8","author":[{"given":"Ainara","family":"Otamendi","sequence":"first","affiliation":[{"name":"Laboratory of Biology, Department of Applied Chemistry, Faculty of Chemistry, University of The Basque Country, 20018 San Sebastian, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5873-6059","authenticated-orcid":false,"given":"Eduardo A.","family":"Espeso","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, Centro de Investigaciones Biol\u00f3gicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9786-6091","authenticated-orcid":false,"given":"Oier","family":"Etxebeste","sequence":"additional","affiliation":[{"name":"Laboratory of Biology, Department of Applied Chemistry, Faculty of Chemistry, University of The Basque Country, 20018 San Sebastian, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,11,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1128\/microbiolspec.FUNK-0052-2016","article-title":"Fungal diversity revisited: 2.2 to 3.8 million species","volume":"5","author":"Hawksworth","year":"2017","journal-title":"Microbiol. Spectr."},{"key":"ref_2","unstructured":"Kirk, P., Cannon, P., Minter, D., and Stalpers, J. (2008). Dictionary of the Fungi, CAN International. [10th ed.]."},{"key":"ref_3","first-page":"1","article-title":"Rewiring of transcriptional networks as a major event leading to the diversity of asexual multicellularity in fungi","volume":"7","author":"Etxebeste","year":"2019","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.simyco.2018.10.002","article-title":"Evolution of asexual and sexual reproduction in the aspergilli","volume":"91","author":"Chen","year":"2018","journal-title":"Stud. Mycol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s13059-017-1151-0","article-title":"Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus","volume":"18","author":"Riley","year":"2017","journal-title":"Genome Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.mib.2012.09.006","article-title":"Genetic control of asexual sporulation in filamentous fungi","volume":"15","author":"Park","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"K\u00fces, U., and Fischer, R. (2006). Asexual sporulation in mycelial fungi. Growth, Differentiation and Sexuality, Springer.","DOI":"10.1007\/3-540-28135-5"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Wendland, J. (2016). Asexual sporulation in Agaricomycetes. Growth, Differentiation and Sexuality, Springer International Publishing.","DOI":"10.1007\/978-3-319-25844-7"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/BF01637246","article-title":"Ultrastructural analysis of conidiophore development in the fungus Aspergillus nidulans using freeze-substitution","volume":"144","author":"Mims","year":"1988","journal-title":"Protoplasma"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/MMBR.62.1.35-54.1998","article-title":"Asexual sporulation in Aspergillus nidulans","volume":"62","author":"Adams","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_11","first-page":"1","article-title":"Aspergillus nidulans in the post-genomic era: A top-model filamentous fungus for the study of signaling and homeostasis mechanisms","volume":"2","author":"Etxebeste","year":"2019","journal-title":"Int. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1007\/s00294-015-0556-0","article-title":"Apical control of conidiation in Aspergillus nidulans","volume":"62","author":"Espeso","year":"2016","journal-title":"Curr. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Hoffmeister, D., and Gressler, M. (2019). Developmental Decisions in Aspergillus nidulans. Biology of the Fungal Cell, Springer International Publishing.","DOI":"10.1007\/978-3-030-05448-9"},{"key":"ref_14","first-page":"1","article-title":"Developmental regulators FlbE\/D orchestrate the polarity site-to-nucleus dynamics of the fungal bZIP transcription factor FlbB","volume":"5","author":"Otamendi","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/mmi.13156","article-title":"Tip-to-nucleus migration dynamics of the asexual development regulator FlbB in vegetative cells","volume":"98","author":"Cortese","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1111\/j.1365-2958.2010.07063.x","article-title":"The concerted action of bZip and cMyb transcription factors FlbB and FlbD induces brlA expression and asexual development in Aspergillus nidulans","volume":"75","author":"Garzia","year":"2010","journal-title":"Mol. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1128\/EC.00207-07","article-title":"Basic-zipper-type transcription factor FlbB controls asexual development in Aspergillus nidulans","volume":"7","author":"Etxebeste","year":"2008","journal-title":"Eukaryot. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1021\/cb200455u","article-title":"Signaling the Induction of Sporulation Involves the Interaction of Two Secondary Metabolites in Aspergillus nidulans","volume":"7","author":"Nieto","year":"2012","journal-title":"ACS Chem. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1099\/00221287-141-1-21","article-title":"Starvation stress modulates the expression of the Aspergillus nidulans brlA regulatory gene","volume":"141","author":"Skromne","year":"1995","journal-title":"Microbiology"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Etxebeste, O., Herrero-Garc\u00eda, E., Cortese, M.S., Garzia, A., Oiartzabal-Arano, E., de los R\u00edos, V., Ugalde, U., and Espeso, E.A. (2012). GmcA is a putative glucose-methanol-choline oxidoreductase required for the induction of asexual development in Aspergillus nidulans. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040292"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1111\/j.1365-2958.2009.06804.x","article-title":"The bZIP-type transcription factor FIbB regulates distinct morphogenetic stages of colony formation in Aspergillus nidulans","volume":"73","author":"Etxebeste","year":"2009","journal-title":"Mol. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1006\/fgbi.2000.1236","article-title":"Polarity-defective mutants of Aspergillus nidulans","volume":"31","author":"Osherov","year":"2000","journal-title":"Fungal Genet. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0926-6593(66)80120-0","article-title":"The induction and repression of nitrate reductase in the fungus Aspergillus nidulans","volume":"113","author":"Cove","year":"1966","journal-title":"Biochim. Biophys. Acta Enzymol. Biol. Oxid."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/genetics\/52.1.217","article-title":"Origins of translocations in Aspergillus nidulans","volume":"52","year":"1965","journal-title":"Genetics"},{"key":"ref_25","first-page":"1784","article-title":"An ordered pathway for the assembly of fungal ESCRT-containing ambient pH signalling complexes at the plasma membrane","volume":"125","author":"Galindo","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.1365-2958.2008.06520.x","article-title":"Aspergillus nidulans FlbE is an upstream developmental activator of conidiation functionally associated with the putative transcription factor FlbB","volume":"71","author":"Garzia","year":"2009","journal-title":"Mol. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.fgb.2005.09.004","article-title":"Tracing the endocytic pathway of Aspergillus nidulans with FM4-64","volume":"42","year":"2005","journal-title":"Fungal Genet. Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1091\/mbc.e09-03-0254","article-title":"Organization and dynamics of the Aspergillus nidulans Golgi during apical extension and mitosis","volume":"20","author":"Pantazopoulou","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1128\/EC.3.5.1359-1362.2004","article-title":"Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans","volume":"3","author":"Yang","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0378-1119(83)90191-9","article-title":"Transformation by integration in Aspergillus nidulans","volume":"26","author":"Tilburn","year":"1983","journal-title":"Gene"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1038\/nprot.2006.405","article-title":"Fusion PCR and gene targeting in Aspergillus nidulans","volume":"1","author":"Szewczyk","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1038\/nprot.2007.112","article-title":"Genetic manipulation of Aspergillus nidulans: Meiotic progeny for genetic analysis and strain construction","volume":"2","author":"Todd","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/nprot.2007.113","article-title":"Genetic manipulation of Aspergillus nidulans: Heterokaryons and diploids for dominance, complementation and haploidization analyses","volume":"2","author":"Todd","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_34","first-page":"413","article-title":"The use of benlate for distinguishing between haploid and diploid strains of Aspergillus nidulans and Aspergillus terreus","volume":"100","author":"Upshall","year":"1977","journal-title":"Microbiology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1093\/nar\/gky1100","article-title":"InterPro in 2019: Improving coverage, classification and access to protein sequence annotations","volume":"47","author":"Mitchell","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1093\/nar\/gkz239","article-title":"Interactive Tree Of Life (iTOL) v4: Recent updates and new developments","volume":"47","author":"Letunic","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: Homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1093\/nar\/gky300","article-title":"DynaMut: Predicting the impact of mutations on protein conformation, flexibility and stability","volume":"46","author":"Rodrigues","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1128\/EC.00119-10","article-title":"Endocytic machinery protein SlaB is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of Aspergillus nidulans","volume":"9","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1534\/genetics.115.174342","article-title":"Beyond asexual development: Modifications in the gene expression profile caused by the absence of the Aspergillus nidulans transcription factor FlbB","volume":"199","author":"Garzia","year":"2015","journal-title":"Genetics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1128\/EC.00274-12","article-title":"Transcriptional changes in the transition from vegetative cells to asexual development in the model fungus Aspergillus nidulans","volume":"12","author":"Garzia","year":"2013","journal-title":"Eukaryot. Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00294-009-0286-2","article-title":"AoxA is a major peroxisomal long chain fatty acyl-CoA oxidase required for \u03b2-oxidation in A. nidulans","volume":"56","author":"Reiser","year":"2010","journal-title":"Curr. Genet."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1128\/EC.00371-08","article-title":"Protein O-Mannosyltransferases B and C support hyphal development and differentiation in Aspergillus nidulans","volume":"8","author":"Goto","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1128\/EC.00040-09","article-title":"Aspergillus nidulans Protein O-Mannosyltransferases play roles in cell wall integrity and developmental patterning","volume":"8","author":"Kriangkripipat","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1007\/s00294-018-0816-x","article-title":"Protein O-mannosyltransferases are required for sterigmatocystin production and developmental processes in Aspergillus nidulans","volume":"64","author":"Le","year":"2018","journal-title":"Curr. Genet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/j.sbi.2003.09.003","article-title":"O-mannosyl glycans: From yeast to novel associations with human disease","volume":"13","author":"Willer","year":"2003","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1016\/j.bbamcr.2013.02.008","article-title":"Protein O-mannosylation: What we have learned from baker\u2019s yeast","volume":"1833","author":"Loibl","year":"2013","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Markina-I\u00f1arrairaegui, A., Pantazopoulou, A., Espeso, E.A., and Pe\u00f1alva, M.A. (2013). The Aspergillus nidulans peripheral ER: Disorganization by ER stress and persistence during mitosis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067154"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"118551","DOI":"10.1016\/j.bbamcr.2019.118551","article-title":"Identification of the guanine nucleotide exchange factor for SAR1 in the filamentous fungal model Aspergillus nidulans","volume":"1866","author":"Pinar","year":"2019","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/0012-1606(80)90349-8","article-title":"Developmental gene regulation in Aspergillus nidulans","volume":"78","author":"Timberlake","year":"1980","journal-title":"Dev. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1099\/00221287-69-2-261","article-title":"A quantitative survey of conidiation mutants in Aspergillus nidulans","volume":"69","author":"Martinelli","year":"1971","journal-title":"J. Gen. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.fgb.2008.12.003","article-title":"The 2008 update of the Aspergillus nidulans genome annotation: A community effort","volume":"46","author":"Wortman","year":"2009","journal-title":"Fungal Genet Biol"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.cell.2015.04.014","article-title":"Making Sense of Transcription Networks","volume":"161","author":"Sorrells","year":"2015","journal-title":"Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.copbio.2015.02.001","article-title":"How do regulatory networks evolve and expand throughout evolution?","volume":"34","author":"Voordeckers","year":"2015","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Dai, Z. (2019). Transcription Factors indirectly regulate genes through nuclear colocalization. Cells, 8.","DOI":"10.3390\/cells8070754"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e00068-17","DOI":"10.1128\/MMBR.00068-17","article-title":"Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures","volume":"82","author":"Riquelme","year":"2018","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1128\/EC.05080-11","article-title":"Putative stress sensors WscA and WscB are involved in hypo-osmotic and acidic pH stress tolerance in Aspergillus nidulans","volume":"10","author":"Futagami","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1111\/j.1365-2958.2007.06021.x","article-title":"Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans","volume":"66","author":"Virag","year":"2007","journal-title":"Mol. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.fob.2014.03.006","article-title":"Aspergillus nidulans Pmts form heterodimers in all pairwise combinations","volume":"4","author":"Kriangkripipat","year":"2014","journal-title":"FEBS Open Bio."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/8\/12\/1520\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:37:44Z","timestamp":1760189864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/8\/12\/1520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,26]]},"references-count":63,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,12]]}},"alternative-id":["cells8121520"],"URL":"https:\/\/doi.org\/10.3390\/cells8121520","relation":{},"ISSN":["2073-4409"],"issn-type":[{"type":"electronic","value":"2073-4409"}],"subject":[],"published":{"date-parts":[[2019,11,26]]}}}