{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:25:47Z","timestamp":1790749547601,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009060","name":"Sidaction","doi-asserted-by":"publisher","award":["10744, 12152"],"award-info":[{"award-number":["10744, 12152"]}],"id":[{"id":"10.13039\/100009060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003323","name":"Agence Nationale de Recherches sur le Sida et les H\u00e9patites Virales","doi-asserted-by":"publisher","award":["N17020DR, ECTZ103291"],"award-info":[{"award-number":["N17020DR, ECTZ103291"]}],"id":[{"id":"10.13039\/501100003323","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programme Investissements d\u2019Avenir","award":["ANR-11-INBS-0008 infrastructure and ANR-10-EQPX-02-01 FlowCyTech facility (IDMIT)"],"award-info":[{"award-number":["ANR-11-INBS-0008 infrastructure and ANR-10-EQPX-02-01 FlowCyTech facility (IDMIT)"]}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["EQU201903007868"],"award-info":[{"award-number":["EQU201903007868"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Background: Aging is characterized by adipose tissue senescence, inflammation, and fibrosis, with trunk fat accumulation. Aging HIV-infected patients have a higher risk of trunk fat accumulation than uninfected individuals\u2014suggesting that viral infection has a role in adipose tissue aging. We previously demonstrated that HIV\/SIV infection and the Tat and Nef viral proteins were responsible for adipose tissue fibrosis and impaired adipogenesis. We hypothesized that SIV\/HIV infection and viral proteins could induce adipose tissue senescence and thus lead to adipocyte dysfunctions. Methods: Features of tissue senescence were evaluated in subcutaneous and visceral adipose tissues of SIV-infected macaques and in human adipose stem cells (ASCs) exposed to Tat or Nef for up to 30 days. Results: p16 expression and p53 activation were higher in adipose tissue of SIV-infected macaques than in control macaques, indicating adipose tissue senescence. Tat and Nef induced higher senescence in ASCs, characterized by higher levels of senescence-associated beta-galactosidase activity, p16 expression, and p53 activation vs. control cells. Treatment with Tat and Nef also induced oxidative stress and mitochondrial dysfunction. Prevention of oxidative stress (using N-acetyl-cysteine) reduced senescence in ASCs. Adipocytes having differentiated from Nef-treated ASCs displayed alterations in adipogenesis with lower levels of triglyceride accumulation and adipocyte marker expression and secretion, and insulin resistance. Conclusion: HIV\/SIV promotes adipose tissue senescence, which in turn may alter adipocyte function and contribute to insulin resistance.<\/jats:p>","DOI":"10.3390\/cells9040854","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T11:57:14Z","timestamp":1585828634000},"page":"854","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["SIV Infection and the HIV Proteins Tat and Nef Induce Senescence in Adipose Tissue and Human Adipose Stem Cells, Resulting in Adipocyte Dysfunction"],"prefix":"10.3390","volume":"9","author":[{"given":"Jennifer","family":"Gorwood","sequence":"first","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Ejlalmanesh","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1146-6087","authenticated-orcid":false,"given":"Christine","family":"Bourgeois","sequence":"additional","affiliation":[{"name":"Immunology of Viral infections and Autoimmune Diseases, IDMIT Department, IBFJ, U1184, INSERM-CEA-Universit\u00e9 Paris Sud 11, F-92260 Fontenay-Aux-Roses and F-94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Mantecon","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Rose","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9529-2648","authenticated-orcid":false,"given":"Michael","family":"Atlan","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"},{"name":"Plastic Surgery Department, Tenon Hospital, AP-HP, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Desjardins","sequence":"additional","affiliation":[{"name":"IDMIT Department, Center for Immunology of Viral Infections and Autoimmune Diseases, Inserm, CEA, Universit\u00e9 Paris Saclay, F-92260 Fontenay-aux-Roses, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Le Grand","sequence":"additional","affiliation":[{"name":"IDMIT Department, Center for Immunology of Viral Infections and Autoimmune Diseases, Inserm, CEA, Universit\u00e9 Paris Saclay, F-92260 Fontenay-aux-Roses, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"F\u00e8ve","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"},{"name":"Diab\u00e9tologie et Reproduction, PRISIS, Service d\u2019Endocrinologie, H\u00f4pital Saint-Antoine, AP-HP, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Lambotte","sequence":"additional","affiliation":[{"name":"Immunology of Viral infections and Autoimmune Diseases, IDMIT Department, IBFJ, U1184, INSERM-CEA-Universit\u00e9 Paris Sud 11, F-92260 Fontenay-Aux-Roses and F-94270 Le Kremlin-Bic\u00eatre, France"},{"name":"Service de M\u00e9decine Interne et Immunologie Clinique, Groupe Hospitalier Universitaire Paris Sud, H\u00f4pital Bic\u00eatre, AP-HP, F-94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Capeau","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9795-549X","authenticated-orcid":false,"given":"V\u00e9ronique","family":"B\u00e9r\u00e9ziat","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche Saint-Antoine-Institut Hospitalo-Universitaire de Cardiom\u00e9tabolisme et Nutrition (ICAN), INSERM, Sorbonne Universit\u00e9, F-75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0700-8286","authenticated-orcid":false,"given":"Claire","family":"Lagathu","sequence":"additional","affiliation":[{"name":"Lipodystrophies, Metabolic and Hormonal Adaptation, and Aging, UMR_S 938, Centre de Recherche 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