{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:35:50Z","timestamp":1788053750256,"version":"build-2784847793"},"reference-count":50,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T00:00:00Z","timestamp":1586995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Zika virus (ZIKV) is an emergent arthropod-borne virus whose outbreak in Brazil has brought major public health problems. Infected individuals have different symptoms, including rash and pruritus, which can be relieved by the administration of antiallergics. In the case of pregnant women, ZIKV can cross the placenta and infect the fetus leading to congenital defects. We have identified that mast cells in the placentae of patients who had Zika during pregnancy can be infected. This led to our investigation on the possible role of mast cells during a ZIKV infection, using the HMC-1 cell line. We analyzed their permissiveness to infection, release of mediators and ultrastructural changes. Flow cytometry detection of ZIKV-NS1 expression 24 h post infection in 45.3% of cells showed that HMC-1 cells are permissive to ZIKV infection. Following infection, \u03b2-hexosaminidase was measured in the supernatant of the cells with a notable release at 30 min. In addition, an increase in TNF-\u03b1, IL-6, IL-10 and VEGF levels were measured at 6 h and 24 h post infection. Lastly, different intracellular changes were observed in an ultrastructural analysis of infected cells. Our findings suggest that mast cells may represent an important source of mediators that can activate other immune cell types during a ZIKV infection, which has the potential to be a major contributor in the spread of the virus in cases of vertical transmission.<\/jats:p>","DOI":"10.3390\/cells9040975","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T13:01:39Z","timestamp":1587042099000},"page":"975","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Zika Virus Infects Human Placental Mast Cells and the HMC-1 Cell Line, and Triggers Degranulation, Cytokine Release and Ultrastructural Changes"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7579-7788","authenticated-orcid":false,"given":"K\u00edssila","family":"Rabelo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551-030, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Jos\u00e9 da Silva","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Jos\u00e9 de","family":"Souza","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Campos, Campos dos Goytacazes, Rio de Janeiro 28035-581, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Paula","family":"Sales","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Campos, Campos dos Goytacazes, Rio de Janeiro 28035-581, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila Maria Barbosa de","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia Virol\u00f3gica, Biomanguinhos, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela Freitas","family":"Trindade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Tecnologia Virol\u00f3gica, Biomanguinhos, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Torres","family":"Ciambarella","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551-030, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia Recardo","family":"Amorim Tasmo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Inflama\u00e7\u00e3o, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Louren\u00e7o","family":"Diaz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Inflama\u00e7\u00e3o, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Jos\u00e9 de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551-030, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia Pereira de Oliveira","family":"Duarte","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marciano Viana","family":"Paes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Interdisciplinar de Pesquisas M\u00e9dicas, Instituto Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1056\/NEJMra1808246","article-title":"Zika Virus Infection\u2014After the Pandemic","volume":"381","author":"Musso","year":"2019","journal-title":"N. 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