{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T22:08:59Z","timestamp":1782511739226,"version":"3.54.5"},"reference-count":49,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"undefined  &lt;span style=&quot;color:gray;font-size:10px;&quot;&gt;undefined&lt;\/span&gt;","award":["18K15923"],"award-info":[{"award-number":["18K15923"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Malignant pleural mesothelioma (MPM) has extremely limited treatment despite a poor prognosis. Moreover, molecular targeted therapy for MPM has not yet been implemented; thus, a new targeted therapy is highly desirable. Near-infrared photoimmunotherapy (NIR-PIT) is a recently developed cancer therapy that combines the specificity of antibodies for targeting tumors with toxicity induced by the photoabsorber after exposure to NIR-light. In this study, we developed a new phototherapy targeting podoplanin (PDPN) for MPM with the use of both NIR-PIT and an anti-PDPN antibody, NZ-1. An antibody\u2013photosensitizer conjugate consisting of NZ-1 and phthalocyanine dye was synthesized. In vitro NIR-PIT-induced cytotoxicity was measured with both dead cell staining and luciferase activity on various MPM cell lines. In vivo NIR-PIT was examined in both the flank tumor and orthotopic mouse model with in vivo real-time imaging. In vitro NIR-PIT-induced cytotoxicity was NIR-light dose dependent. In vivo NIR-PIT led to significant reduction in both tumor volume and luciferase activity in a flank model (p &lt; 0.05, NIR-PIT group versus NZ-1-IR700 group). The PDPN-targeted NIR-PIT resulted in a significant antitumor effect in an MPM orthotopic mouse model (p &lt; 0.05, NIR-PIT group versus NZ-1-IR700 group). This study suggests that PDPN-targeted NIR-PIT could be a new promising treatment for MPM.<\/jats:p>","DOI":"10.3390\/cells9041019","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T05:48:52Z","timestamp":1587448132000},"page":"1019","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Targeted Phototherapy for Malignant Pleural Mesothelioma: Near-Infrared Photoimmunotherapy Targeting Podoplanin"],"prefix":"10.3390","volume":"9","author":[{"given":"Yuko","family":"Nishinaga","sequence":"first","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhide","family":"Sato","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"},{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"},{"name":"Nagoya University Institute for Advanced Research, S-YLC, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotoshi","family":"Yasui","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunichi","family":"Taki","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuomi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misae","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"},{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rena","family":"Endo","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"},{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Koike","sequence":"additional","affiliation":[{"name":"Respiratory Medicine, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"},{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Kuramoto","sequence":"additional","affiliation":[{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shota","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9352-1520","authenticated-orcid":false,"given":"Hiroshi","family":"Yukawa","sequence":"additional","affiliation":[{"name":"B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)\/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research, Nagoya 466-8550, Japan"},{"name":"Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya 464-8601, Japan"},{"name":"Department of Biomolecular Engineering, Nagoya University Graduate School of Engineering, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Baba","sequence":"additional","affiliation":[{"name":"Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya 464-8601, Japan"},{"name":"Department of Biomolecular Engineering, Nagoya University Graduate School of Engineering, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"K. Kaneko","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toyofumi F.","family":"Chen-Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1019-4112","authenticated-orcid":false,"given":"Hisataka","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1088, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5385-8201","authenticated-orcid":false,"given":"Yukinari","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai 980-8575, Japan"},{"name":"New Industry Creation Hatchery Center, Tohoku University, Sendai 980-8575, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"National Hospital Organization, Nagoya Medical Center, Nagoya 460-0001, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/bmb\/ldp047","article-title":"Malignant mesothelioma","volume":"93","author":"Rudd","year":"2010","journal-title":"Br. 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