{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:37:33Z","timestamp":1770223053250,"version":"3.49.0"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["132484\/BR\/VO-R\u00e9f INSERM:131589A10"],"award-info":[{"award-number":["132484\/BR\/VO-R\u00e9f INSERM:131589A10"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013945","name":"Institut de Recherches Servier","doi-asserted-by":"publisher","award":["Modulation pharmacologique de la fuite calcique du RE au sein des cardiomyocytes soumis \u00e0 l'hypoxie-r\u00e9oxyg\u00e9nation"],"award-info":[{"award-number":["Modulation pharmacologique de la fuite calcique du RE au sein des cardiomyocytes soumis \u00e0 l'hypoxie-r\u00e9oxyg\u00e9nation"]}],"id":[{"id":"10.13039\/501100013945","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-IBHU-004"],"award-info":[{"award-number":["ANR-10-IBHU-004"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>During myocardial infarction, dysregulation of Ca2+ homeostasis between the reticulum, mitochondria, and cytosol occurs in cardiomyocytes and leads to cell death. Ca2+ leak channels are thought to be key regulators of the reticular Ca2+ homeostasis and cell survival. The present study aimed to determine whether a particular reticular Ca2+ leak channel, the translocon, also known as translocation channel, could be a relevant target against ischemia\/reperfusion-mediated heart injury. To achieve this objective, we first used an intramyocardial adenoviral strategy to express biosensors in order to assess Ca2+ variations in freshly isolated adult mouse cardiomyocytes to show that translocon is a functional reticular Ca2+ leak channel. Interestingly, translocon activation by puromycin mobilized a ryanodine receptor (RyR)-independent reticular Ca2+ pool and did not affect the excitation\u2013concentration coupling. Second, puromycin pretreatment decreased mitochondrial Ca2+ content and slowed down the mitochondrial permeability transition pore (mPTP) opening and the rate of cytosolic Ca2+ increase during hypoxia. Finally, this translocon pre-activation also protected cardiomyocytes after in vitro hypoxia reoxygenation and reduced infarct size in mice submitted to in vivo ischemia-reperfusion. Altogether, our report emphasizes the role of translocon in cardioprotection and highlights a new paradigm in cardioprotection by functionally uncoupling the RyR-dependent Ca2+ stores and translocon-dependent Ca2+ stores.<\/jats:p>","DOI":"10.3390\/cells9051319","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T03:29:10Z","timestamp":1590463750000},"page":"1319","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Acute Induction of Translocon-Mediated Ca2+ Leak Protects Cardiomyocytes Against Ischemia\/Reperfusion Injury"],"prefix":"10.3390","volume":"9","author":[{"given":"Ribal","family":"Al-Mawla","sequence":"first","affiliation":[{"name":"Univ Lyon, CarMeN Laboratory, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, 69500 Bron, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mallory","family":"Ducrozet","sequence":"additional","affiliation":[{"name":"Univ Lyon, CarMeN Laboratory, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, 69500 Bron, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nolwenn","family":"Tessier","sequence":"additional","affiliation":[{"name":"Univ Lyon, CarMeN Laboratory, INSERM, INRA, INSA Lyon, Universit\u00e9 Claude Bernard Lyon 1, 69500 Bron, France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier EST, D\u00e9partement de Cardiologie, IHU-OPERA B\u00e2timent B13, 69500 Bron, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucille","family":"Pa\u00efta","sequence":"additional","affiliation":[{"name":"Univ Lyon, CarMeN Laboratory, INSERM, 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Fr. D\u2019anesthesie Reanim."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1056\/NEJMra1112570","article-title":"A tale of coronary artery disease and myocardial infarction","volume":"366","author":"Nabel","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/nature13909","article-title":"Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS","volume":"515","author":"Chouchani","year":"2014","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"H2260","DOI":"10.1152\/ajpheart.01153.2004","article-title":"Defective calcium handling in cardiomyocytes isolated from hearts subjected to ischemia-reperfusion","volume":"288","author":"Saini","year":"2005","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.carpath.2005.03.006","article-title":"Myocardial ischemia and reperfusion injury","volume":"14","author":"Buja","year":"2005","journal-title":"Cardiovasc. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11389","DOI":"10.1073\/pnas.1513047112","article-title":"Mitochondrial calcium overload is a key determinant in heart failure","volume":"112","author":"Santulli","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","first-page":"68","article-title":"Myocardial necrosis induced by temporary occlusion of a coronary artery in the dog","volume":"70","author":"Jennings","year":"1960","journal-title":"Arch. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1124\/pr.113.008300","article-title":"Interaction of risk factors, comorbidities, and comedications with ischemia\/reperfusion injury and cardioprotection by preconditioning, postconditioning, and remote conditioning","volume":"66","author":"Ferdinandy","year":"2014","journal-title":"Pharmacol. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1161\/CIRCULATIONAHA.113.001225","article-title":"Depressing mitochondria-reticulum interactions protects cardiomyocytes from lethal hypoxia-reoxygenation injury","volume":"128","author":"Paillard","year":"2013","journal-title":"Circulation"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/cdd.2015.101","article-title":"The SR\/ER-mitochondria calcium crosstalk is regulated by GSK3\u03b2 during reperfusion injury","volume":"23","author":"Gomez","year":"2016","journal-title":"Cell Death Differ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-94-007-2888-2_1","article-title":"The Regulation of a Cell\u2019s Ca2+ Signaling Toolkit: The Ca2+ Homeostasome","volume":"Volume 740","author":"Islam","year":"2012","journal-title":"Calcium Signaling"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ceca.2010.02.001","article-title":"Intracellular Ca2+ storage in health and disease: A dynamic equilibrium","volume":"47","author":"Sammels","year":"2010","journal-title":"Cell Calcium"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"a004242","DOI":"10.1101\/cshperspect.a004242","article-title":"Calcium Signaling in Cardiac Myocytes","volume":"3","author":"Fearnley","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1007\/s00395-016-0588-8","article-title":"Ischaemic conditioning and targeting reperfusion injury: A 30 year voyage of discovery","volume":"111","author":"Hausenloy","year":"2016","journal-title":"Basic Res. Cardiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1097\/ALN.0000000000000876","article-title":"Involvement of Cyclophilin D and Calcium in Isoflurane-induced Preconditioning","volume":"123","author":"Teixeira","year":"2015","journal-title":"Anesthesiology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e2238","DOI":"10.1038\/cddis.2016.139","article-title":"Hearts deficient in both Mfn1 and Mfn2 are protected against acute myocardial infarction","volume":"7","author":"Hall","year":"2016","journal-title":"Cell Death Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1056\/NEJMoa1505489","article-title":"Cyclosporine before PCI in Patients with Acute Myocardial Infarction","volume":"373","author":"Cung","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"228","DOI":"10.4161\/chan.5.3.15314","article-title":"Sec61 complexes form ubiquitous ER Ca2+ leak channels","volume":"5","author":"Lang","year":"2011","journal-title":"Channels"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7214","DOI":"10.1073\/pnas.97.13.7214","article-title":"Homologs of the yeast Sec complex subunits Sec62p and Sec63p are abundant proteins in dog pancreas microsomes","volume":"97","author":"Tyedmers","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/S0092-8674(00)80235-4","article-title":"The aqueous pore through the translocon has a diameter of 40-60 A during cotranslational protein translocation at the ER membrane","volume":"89","author":"Hamman","year":"1997","journal-title":"Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1085\/jgp.102.6.1107","article-title":"Probing the structure of the conduction pathway of the sheep cardiac sarcoplasmic reticulum calcium-release channel with permeant and impermeant organic cations","volume":"102","author":"Tinker","year":"1993","journal-title":"J. Gen. Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1016\/S0006-3495(02)75544-6","article-title":"Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials","volume":"82","author":"Mead","year":"2002","journal-title":"Biophys. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1113\/jphysiol.1991.sp018676","article-title":"Monovalent cation conductance in the ryanodine receptor-channel of sheep cardiac muscle sarcoplasmic reticulum","volume":"439","author":"Lindsay","year":"1991","journal-title":"J. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1083\/jcb.200409174","article-title":"The molecular mechanisms underlying BiP-mediated gating of the Sec61 translocon of the endoplasmic reticulum","volume":"168","author":"Alder","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1096\/fj.12-218875","article-title":"Modulation of ER stress and apoptosis by endoplasmic reticulum calcium leak via translocon during unfolded protein response: Involvement of GRP78","volume":"27","author":"Hammadi","year":"2013","journal-title":"FASEB J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1016\/j.str.2008.05.003","article-title":"Single copies of Sec61 and TRAP associate with a non-translating mammalian ribosome","volume":"16","author":"Hegde","year":"2008","journal-title":"Structure"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1126\/science.1178535","article-title":"Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome","volume":"326","author":"Becker","year":"2009","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4397","DOI":"10.1074\/jbc.M207295200","article-title":"The Permeability of the Endoplasmic Reticulum Is Dynamically Coupled to Protein Synthesis","volume":"278","author":"Roy","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_29","first-page":"1958","article-title":"Different effects of Sec61\u03b1, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells","volume":"125","author":"Lang","year":"2012","journal-title":"J. Cell Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26479","DOI":"10.1074\/jbc.M201845200","article-title":"Basal and physiological Ca2+ leak from the endoplasmic reticulum of pancreatic acinar cells. Second messenger-activated channels and translocons","volume":"277","author":"Lomax","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4135","DOI":"10.1242\/jcs.01274","article-title":"Ribosome-translocon complex mediates calcium leakage from endoplasmic reticulum stores","volume":"117","author":"Coppenolle","year":"2004","journal-title":"J. Cell Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1096\/fj.05-5254fje","article-title":"Passive calcium leak via translocon is a first step for iPLA2-pathway regulated store operated channels activation","volume":"20","author":"Flourakis","year":"2006","journal-title":"FASEB J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.ceca.2013.01.002","article-title":"Luminal Ca2+ depletion during the unfolded protein response in Xenopus oocytes: Cause and consequence","volume":"53","author":"Paredes","year":"2013","journal-title":"Cell Calcium"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.bbrc.2017.04.106","article-title":"Influence of ER leak on resting cytoplasmic Ca2+ and receptor-mediated Ca2+ signalling in human macrophage","volume":"487","author":"Layhadi","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.abb.2007.03.039","article-title":"Both translocon and a cation channel are involved in the passive Ca2+ leak from the endoplasmic reticulum: A mechanistic study on rat liver microsomes","volume":"462","author":"Giunti","year":"2007","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/emboj.2010.284","article-title":"Interaction of calmodulin with Sec61\u03b1 limits Ca2+ leakage from the endoplasmic reticulum","volume":"30","author":"Erdmann","year":"2011","journal-title":"EMBO J."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cassel, R., Ducreux, S., Alam, M.R., Dingreville, F., Berl\u00e9, C., Burda-Jacob, K., Chauvin, M.A., Chikh, K., Pa\u00efta, L., and Al-Mawla, R. (2016). Protection of Human Pancreatic Islets from Lipotoxicity by Modulation of the Translocon. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0148686"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"17002","DOI":"10.1038\/sigtrans.2017.2","article-title":"Let\u2019s talk about Secs: Sec61, Sec62 and Sec63 in signal transduction, oncology and personalized medicine","volume":"2","author":"Linxweiler","year":"2017","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Linxweiler, M., Schorr, S., Sch\u00e4uble, N., Jung, M., Linxweiler, J., Langer, F., Sch\u00e4fers, H.-J., Cavali\u00e9, A., Zimmermann, R., and Greiner, M. (2013). Targeting cell migration and the endoplasmic reticulum stress response with calmodulin antagonists: A clinically tested small molecule phenocopy of SEC62 gene silencing in human tumor cells. BMC Cancer, 13.","DOI":"10.1186\/1471-2407-13-574"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9105","DOI":"10.1158\/0008-5472.CAN-09-2775","article-title":"Glioblastoma Proto-Oncogene SEC61\u03b3 Is Required for Tumor Cell Survival and Response to Endoplasmic Reticulum Stress","volume":"69","author":"Lu","year":"2009","journal-title":"Cancer Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lang, S., Pfeffer, S., Lee, P.-H., Cavali\u00e9, A., Helms, V., F\u00f6rster, F., and Zimmermann, R. (2017). An Update on Sec61 Channel Functions, Mechanisms, and Related Diseases. Front. Physiol., 8.","DOI":"10.3389\/fphys.2017.00887"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1038\/nprot.2006.172","article-title":"Measuring calcium signaling using genetically targetable fluorescent indicators","volume":"1","author":"Palmer","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.cmpb.2013.09.004","article-title":"Ca analysis: An Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis","volume":"113","author":"Greensmith","year":"2014","journal-title":"Comput. Methods Programs Biomed."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.devcel.2011.08.008","article-title":"The permeability transition pore controls cardiac mitochondrial maturation and myocyte differentiation","volume":"21","author":"Hom","year":"2011","journal-title":"Dev. Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0006-3495(99)77239-5","article-title":"Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence","volume":"76","author":"Petronilli","year":"1999","journal-title":"Biophys. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"C439","DOI":"10.1152\/ajpcell.00393.2019","article-title":"An innovative sequence of hypoxia-reoxygenation on adult mouse cardiomyocytes in suspension to perform multilabeling analysis by flow cytometry","volume":"318","author":"Paccalet","year":"2019","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"H910","DOI":"10.1152\/ajpheart.00984.2008","article-title":"Translocon closure to Ca2+ leak in proliferating vascular smooth muscle cells","volume":"296","author":"Amer","year":"2009","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1161\/CIRCRESAHA.117.310230","article-title":"Calcium and Excitation-Contraction Coupling in the Heart","volume":"121","author":"Eisner","year":"2017","journal-title":"Circ. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.yjmcc.2014.12.025","article-title":"Transitions of protein traffic from cardiac ER to junctional SR","volume":"81","author":"Sleiman","year":"2015","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1469-7793.2000.00069.x","article-title":"The machinery of local Ca2+ signalling between sarco-endoplasmic reticulum and mitochondria","volume":"529","author":"Madesh","year":"2000","journal-title":"J. Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.yjmcc.2015.03.017","article-title":"Inhibition of myocardial reperfusion injury by ischemic postconditioning requires sirtuin 3-mediated deacetylation of cyclophilin D","volume":"84","author":"Bochaton","year":"2015","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1089\/ars.2010.3486","article-title":"Cardioprotection by endoplasmic reticulum stress-induced autophagy","volume":"14","author":"Petrovski","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/aps.2015.145","article-title":"Endoplasmic reticulum stress: A novel mechanism and therapeutic target for cardiovascular diseases","volume":"37","author":"Liu","year":"2016","journal-title":"Acta Pharmacol. 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