{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:16:35Z","timestamp":1776356195720,"version":"3.51.2"},"reference-count":73,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T00:00:00Z","timestamp":1591747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["NRF-2016R1A2B4009156"],"award-info":[{"award-number":["NRF-2016R1A2B4009156"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["NRF-2018R1D1A1B07044543"],"award-info":[{"award-number":["NRF-2018R1D1A1B07044543"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>In the present study, we investigated the effects of cuprizone on cell death, glial activation, and neuronal plasticity induced by hypothermia after ischemia in gerbils. Food was supplemented with cuprizone at 0.2% ad libitum for eight weeks. At six weeks after diet feeing, gerbils received transient forebrain ischemia with or without hypothermic preconditioning. Cuprizone treatment for 8 weeks increased the number of astrocytes, microglia, and pro-inflammatory cytokine levels in the hippocampus. In addition, cuprizone treatment significantly decreased the number of proliferating cells and neuroblasts in the dentate gyrus. Brain ischemia caused cell death, disruption of myelin basic proteins, and reactive gliosis in CA1. In addition, ischemia significantly increased pro-inflammatory cytokines and the number of proliferating cells and differentiating neuroblasts in the dentate gyrus. In contrast, hypothermic conditioning attenuated these changes in CA1 and the dentate gyrus. However, cuprizone treatment decreased cell survival induced by hypothermic preconditioning after ischemia and increased the number of reactive microglia and astrocytes in CA1 as well as that of macrophages in the subcallosal zone. These changes occurred because the protective effect of hypothermia in ischemic damage was disrupted by cuprizone administration. Furthermore, cuprizone decreased ischemia-induced proliferating cells and neuroblasts in the dentate gyrus.<\/jats:p>","DOI":"10.3390\/cells9061438","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T05:11:46Z","timestamp":1591765906000},"page":"1438","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemia"],"prefix":"10.3390","volume":"9","author":[{"given":"Woosuk","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"},{"name":"Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu Ri","family":"Hahn","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo Young","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Jung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Min","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 05030, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Hyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Whi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0278-2291","authenticated-orcid":false,"given":"Dae Young","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 31151, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung Hoon","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeo Sung","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0533-4638","authenticated-orcid":false,"given":"In Koo","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"138","DOI":"10.2174\/157340310791658785","article-title":"Epidemiology and risk factors of cerebral ischemia and ischemic heart diseases: Similarities and differences","volume":"6","author":"Soler","year":"2010","journal-title":"Curr. 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