{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:50:52Z","timestamp":1790722252671,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T00:00:00Z","timestamp":1595030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["322749"],"award-info":[{"award-number":["322749"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these cell populations become activated and initiate finely-orchestrated crosstalk that promotes myofiber repair and regeneration. Mass cytometry is a powerful and highly multiplexed technique for profiling single-cells. Herein, it was used to dissect the dynamics of cell populations in the skeletal muscle in physiological and pathological conditions. Here, we characterized an antibody panel that could be used to identify most of the cell populations in the muscle interstitial space. By exploiting the mass cytometry resolution, we provided a comprehensive picture of the dynamics of the major cell populations that sensed and responded to acute damage in wild type mice and in a mouse model of Duchenne muscular dystrophy. In addition, we revealed the intrinsic heterogeneity of many of these cell populations.<\/jats:p>","DOI":"10.3390\/cells9071723","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T06:38:55Z","timestamp":1595313535000},"page":"1723","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6453-3031","authenticated-orcid":false,"given":"Lucia Lisa","family":"Petrilli","sequence":"first","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"},{"name":"Department of Onco-hematology, Gene and Cell Therapy\u2014Bambino Ges\u00f9 Children\u2019s Hospital\u2014IRCCS, 00146 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8063-3953","authenticated-orcid":false,"given":"Filomena","family":"Spada","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1300-5479","authenticated-orcid":false,"given":"Alessandro","family":"Palma","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5333-7502","authenticated-orcid":false,"given":"Alessio","family":"Reggio","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0952-1710","authenticated-orcid":false,"given":"Marco","family":"Rosina","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1742-3969","authenticated-orcid":false,"given":"Cesare","family":"Gargioli","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5283-8671","authenticated-orcid":false,"given":"Luisa","family":"Castagnoli","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8372-305X","authenticated-orcid":false,"given":"Claudia","family":"Fuoco","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni","family":"Cesareni","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"},{"name":"Fondazione Santa Lucia Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), 00143 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1089\/hum.1997.8.12-1429","article-title":"Replicative potential and telomere length in human skeletal muscle: Implications for satellite cell-mediated gene therapy","volume":"8","author":"Decary","year":"1997","journal-title":"Hum. Gene Ther."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1083\/jcb.9.2.493","article-title":"Satellite cell of skeletal muscle fibers","volume":"9","author":"Mauro","year":"1961","journal-title":"J. Biophys. Biochem. Cytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1152\/physrev.00043.2011","article-title":"Satellite cells and the muscle stem cell niche","volume":"93","author":"Yin","year":"2013","journal-title":"Physiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3639","DOI":"10.1242\/dev.067595","article-title":"An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration","volume":"138","author":"Lepper","year":"2011","journal-title":"Development"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3647","DOI":"10.1242\/dev.067587","article-title":"Pax7-expressing satellite cells are indispensable for adult skeletal muscle regeneration","volume":"138","author":"Sambasivan","year":"2011","journal-title":"Development"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/S0092-8674(00)00066-0","article-title":"Pax7 is required for the specification of myogenic satellite cells","volume":"102","author":"Seale","year":"2000","journal-title":"Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.cell.2013.02.053","article-title":"Type 2 innate signals stimulate fibro\/adipogenic progenitors to facilitate muscle regeneration","volume":"153","author":"Heredia","year":"2013","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/ncb2015","article-title":"Muscle injury activates resident fibro\/adipogenic progenitors that facilitate myogenesis","volume":"12","author":"Joe","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3654","DOI":"10.1242\/jcs.086629","article-title":"Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle","volume":"124","author":"Uezumi","year":"2011","journal-title":"J. Cell Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1242\/dev.064162","article-title":"Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration","volume":"138","author":"Murphy","year":"2011","journal-title":"Development"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-018-06068-6","article-title":"Dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy","volume":"9","author":"Malecova","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0140-6736(02)07815-7","article-title":"The muscular dystrophies","volume":"359","author":"Emery","year":"2002","journal-title":"Lancet"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"1Marden, F.A., Connolly, A.M., Siegel, M.J., and Rubin, D.A. (2004). Compositional analysis of muscle in boys with Duchenne muscular dystrophy using MR imaging. Skelet. Radiol., 34, 140\u2013148.","DOI":"10.1007\/s00256-004-0825-3"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s00441-015-2343-0","article-title":"Connective tissue cells expressing fibro\/adipogenic progenitor markers increase under chronic damage: Relevance in fibroblast-myofibroblast differentiation and skeletal muscle fibrosis","volume":"364","author":"Contreras","year":"2016","journal-title":"Cell Tissue Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-10438-z","article-title":"Fibroadipogenic progenitors are responsible for muscle loss in limb girdle muscular dystrophy 2B","volume":"10","author":"Hogarth","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/s41556-018-0151-y","article-title":"Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis","volume":"20","author":"Madaro","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s13539-012-0098-y","article-title":"Sources for skeletal muscle repair: From satellite cells to reprogramming","volume":"4","author":"Sirabella","year":"2013","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s40610-015-0025-z","article-title":"Contributions of muscle-resident progenitor cells to homeostasis and disease","volume":"1","author":"Dey","year":"2015","journal-title":"Curr. Mol. Biol. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"E453","DOI":"10.1152\/ajpendo.00553.2012","article-title":"Cross-talk between skeletal muscle and immune cells: Muscle-derived mediators and metabolic implications","volume":"304","author":"Pillon","year":"2013","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.molcel.2019.02.026","article-title":"High-dimensional single-cell cartography reveals novel skeletal muscle-resident cell populations","volume":"74","author":"Giordani","year":"2019","journal-title":"Mol. Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/978-1-4939-6771-1_10","article-title":"Single cell gene expression profiling of skeletal muscle-derived cells","volume":"1556","author":"Gatto","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"ref_22","first-page":"229","article-title":"Single-cell transcriptional profiles in human skeletal muscle","volume":"10","author":"Rubenstein","year":"2020","journal-title":"Nat. Publ. Group"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"dev174177","DOI":"10.1242\/dev.174177","article-title":"Single cell analysis of adult mouse skeletal muscle stem cells in homeostatic and regenerative conditions","volume":"146","author":"Juan","year":"2019","journal-title":"Development"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e201900437","DOI":"10.26508\/lsa.201900437","article-title":"Fibro-adipogenic progenitors of dystrophic mice are insensitive to NOTCH regulation of adipogenesis","volume":"2","author":"Marinkovic","year":"2019","journal-title":"Life Sci. Alliance"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.nbt.2015.12.007","article-title":"Characterization by mass cytometry of different methods for the preparation of muscle mononuclear cells","volume":"33","author":"Spada","year":"2016","journal-title":"New Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/cyto.a.22271","article-title":"Normalization of mass cytometry data with bead standards","volume":"83","author":"Finck","year":"2013","journal-title":"Cytom. Part"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kotecha, N., Krutzik, P.O., and Irish, J.M. (2010). Web-based analysis and publication of flow cytometry experiments. Curr. Protoc. Cytom.","DOI":"10.1002\/0471142956.cy1017s53"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chen, H., Lau, M.C., Wong, M.T., Newell, E.W., and Chen, J. (2016). Cytofkit: A bioconductor package for an integrated mass cytometry data analysis pipeline. PLoS Comput. Biol., 12.","DOI":"10.1371\/journal.pcbi.1005112"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1074\/jbc.M113.493270","article-title":"Mitochondrial alterations and oxidative stress in an acute transient mouse model of muscle degeneration: Implications for muscular dystrophy and related muscle pathologies","volume":"289","author":"Gayathri","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/0022-510X(74)90041-0","article-title":"Changes in motor end-plates resulting from muscle fibre necrosis and regeneration. A light and electron microscopic study of the effects of the depolarizing fraction (cardiotoxin) of Dendroaspis jamesoni venom","volume":"21","author":"Duchen","year":"1974","journal-title":"J. Neurol. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1006\/jmcc.1997.0511","article-title":"Cobra venom cardiotoxin induces perturbations of cytosolic calcium homeostasis and hypercontracture in adult rat ventricular myocytes","volume":"29","author":"Wang","year":"1997","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1016\/S0021-9258(18)49909-7","article-title":"Membrane interactions of amphiphilic polypeptides mastoparan, melittin, polymyxin B, and cardiotoxin. Differential inhibition of protein kinase C, Ca2+\/calmodulin-dependent protein kinase II and synaptosomal membrane Na,K-ATPase, and Na+ pump and differentiation of HL60 cells","volume":"266","author":"Raynor","year":"1991","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"32753","DOI":"10.1074\/jbc.271.51.32753","article-title":"Induction of cytosolic Ca2+ elevation mediated by Mas-7 occurs through membrane pore formation","volume":"271","author":"Suh","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1038\/embor.2013.182","article-title":"Cellular dynamics in the muscle satellite cell niche","volume":"14","author":"Bentzinger","year":"2013","journal-title":"EMBO Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1152\/jappl.1994.77.1.290","article-title":"Differential response of macrophage subpopulations to soleus muscle reloading after rat hindlimb suspension","volume":"77","author":"Tidball","year":"1994","journal-title":"J. Appl. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1016\/j.cell.2010.11.039","article-title":"Short telomeres and stem cell exhaustion model Duchenne muscular dystrophy in mdx\/mTR mice","volume":"143","author":"Sacco","year":"2010","journal-title":"Cell"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1002\/ar.1092190305","article-title":"Electron microscopic and autoradiographic characterization of hindlimb muscle regeneration in the mdx mouse","volume":"219","author":"Anderson","year":"1987","journal-title":"Anat. Rec."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/nprot.2015.020","article-title":"Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm","volume":"10","author":"Zunder","year":"2015","journal-title":"Nat. Protoc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nbt.2594","article-title":"VISNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia","volume":"31","author":"Amir","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.3389\/fphys.2019.01216","article-title":"Myo-REG: A portal for signaling interactions in muscle regeneration","volume":"10","author":"Palma","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1084\/jem.20070075","article-title":"Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis","volume":"204","author":"Arnold","year":"2007","journal-title":"J. Exp. Med."},{"key":"ref_42","first-page":"17","article-title":"Degenerating and regenerating skeletal muscles contain several subpopulations of macrophages with distinct spatial and temporal distributions","volume":"188","author":"McLennan","year":"1996","journal-title":"J. Anat."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1189\/jlb.0908579","article-title":"Inflammatory and alternatively activated human macrophages attract vessel-associated stem cells, relying on separate HMGB1- and MMP-9-dependent pathways","volume":"85","author":"Lolmede","year":"2009","journal-title":"J. Leukoc. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1083\/jcb.200212046","article-title":"Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth","volume":"163","author":"Chazaud","year":"2003","journal-title":"J. Cell Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.2353\/ajpath.2010.090884","article-title":"Macrophages protect against muscle atrophy and promote muscle recovery in vivo and in vitro: A mechanism partly dependent on the insulin-like growth factor-1 signaling molecule","volume":"176","author":"Dumont","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"13","DOI":"10.12703\/P6-13","article-title":"The M1 and M2 paradigm of macrophage activation: Time for reassessment","volume":"6","author":"Martinez","year":"2014","journal-title":"F1000Prime Rep."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kharraz, Y., Guerra, J., Mann, C.J., Serrano, A.L., and Mu\u00f1oz-C\u00e1noves, P. (2013). Macrophage plasticity and the role of inflammation in skeletal muscle repair. Mediat. Inflamm.","DOI":"10.1155\/2013\/491497"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/path.4133","article-title":"Macrophage plasticity and polarization in tissue repair and remodelling","volume":"229","author":"Mantovani","year":"2013","journal-title":"J. Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/stem.1288","article-title":"Differentially activated macrophages orchestrate myogenic precursor cell fate during human skeletal muscle regeneration","volume":"31","author":"Saclier","year":"2013","journal-title":"Stem Cells"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"342","DOI":"10.4049\/jimmunol.175.1.342","article-title":"Macrophages sequentially change their functional phenotype in response to changes in microenvironmental influences","volume":"175","author":"Stout","year":"2005","journal-title":"J. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1007\/s00018-014-1819-5","article-title":"Stem cell activation in skeletal muscle regeneration","volume":"72","author":"Fu","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1113\/jphysiol.2002.031450","article-title":"Interactions between neutrophils and macrophages promote macrophage killing of rat muscle cells in vitro","volume":"547","author":"Nguyen","year":"2003","journal-title":"J. Physiol. (Lond.)"},{"key":"ref_53","first-page":"1","article-title":"The basis of muscle regeneration","volume":"2014","year":"2014","journal-title":"Adv. Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s004410050681","article-title":"In vivo angiogenesis in adult rat skeletal muscle: Early changes in capillary network architecture and ultrastructure","volume":"286","author":"Hudlicka","year":"1996","journal-title":"Cell Tissue Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1038\/nbt1334","article-title":"Prospective identification of myogenic endothelial cells in human skeletal muscle","volume":"25","author":"Zheng","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/ncb1542","article-title":"Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells","volume":"9","author":"Dellavalle","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1089\/scd.2012.0647","article-title":"Role of pericytes in skeletal muscle regeneration and fat accumulation","volume":"22","author":"Birbrair","year":"2013","journal-title":"Stem Cells Dev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1091\/mbc.e06-08-0693","article-title":"Muscle satellite cells and endothelial cells: Close neighbors and privileged partners","volume":"18","author":"Christov","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nrm.2016.7","article-title":"Molecular circuitry of stem cell fate in skeletal muscle regeneration, ageing and disease","volume":"17","author":"Almada","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1038\/nm.3918","article-title":"The central role of muscle stem cells in regenerative failure with aging","volume":"21","author":"Blau","year":"2015","journal-title":"Nat. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.immuni.2016.01.009","article-title":"Poor repair of skeletal muscle in aging mice reflects a defect in local, interleukin-33-dependent accumulation of regulatory T cells","volume":"44","author":"Kuswanto","year":"2016","journal-title":"Immunity"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1002\/mus.880181011","article-title":"Age-related differences in regeneration of dystrophic (mdx) and normal muscle in the mouse","volume":"18","author":"Pastoret","year":"1995","journal-title":"Muscle Nerve"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2010.07.002","article-title":"Highly multiparametric analysis by mass cytometry","volume":"361","author":"Ornatsky","year":"2010","journal-title":"J. Immunol. Methods"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.it.2012.02.010","article-title":"A deep profiler\u2019s guide to cytometry","volume":"33","author":"Bendall","year":"2012","journal-title":"Trends Immunol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"6813","DOI":"10.1021\/ac901049w","article-title":"Mass cytometry: Technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry","volume":"81","author":"Bandura","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_66","first-page":"1","article-title":"Adipogenesis of skeletal muscle fibro\/adipogenic progenitors is affected by the WNT5a\/GSK3\/\u03b2-catenin axis","volume":"108","author":"Reggio","year":"2020","journal-title":"Cell Death Differ."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/ncb2014","article-title":"Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle","volume":"12","author":"Uezumi","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nm.3869","article-title":"Nilotinib reduces muscle fibrosis in chronic muscle injury by promoting TNF-mediated apoptosis of fibro\/ adipogenic progenitors","volume":"21","author":"Lemos","year":"2015","journal-title":"Nat. Med."},{"key":"ref_69","first-page":"916","article-title":"The expression of vimentin in satellite cells of regenerating skeletal muscle in vivo","volume":"26","author":"Vater","year":"1994","journal-title":"Histochem. J."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1089\/ten.tea.2010.0553","article-title":"Human muscle-derived cell populations isolated by differential adhesion rates: Phenotype and contribution to skeletal muscle regeneration in Mdx\/SCID mice","volume":"18","author":"Chirieleison","year":"2012","journal-title":"Tissue Eng. 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