{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:01:40Z","timestamp":1791576100114,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T00:00:00Z","timestamp":1595203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["2015FBNB5Y"],"award-info":[{"award-number":["2015FBNB5Y"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004740","name":"Fondazione del Monte di Bologna e Ravenna","doi-asserted-by":"publisher","award":["699bis\/2019"],"award-info":[{"award-number":["699bis\/2019"]}],"id":[{"id":"10.13039\/501100004740","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Associazione Italiana Distrofia Muscolare di Emery Dreifuss (AIDMED) ONLUS","award":["01\/2019"],"award-info":[{"award-number":["01\/2019"]}]},{"name":"Alessandra Proietti ONLUS","award":["01\/2019"],"award-info":[{"award-number":["01\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Lamin A\/C has been implicated in the epigenetic regulation of muscle gene expression through dynamic interaction with chromatin domains and epigenetic enzymes. We previously showed that lamin A\/C interacts with histone deacetylase 2 (HDAC2). In this study, we deepened the relevance and regulation of lamin A\/C-HDAC2 interaction in human muscle cells. We present evidence that HDAC2 binding to lamin A\/C is related to HDAC2 acetylation on lysine 75 and expression of p300-CBP associated factor (PCAF), an acetyltransferase known to acetylate HDAC2. Our findings show that lamin A and farnesylated prelamin A promote PCAF recruitment to the nuclear lamina and lamin A\/C binding in human myoblasts committed to myogenic differentiation, while protein interaction is decreased in differentiating myotubes. Interestingly, PCAF translocation to the nuclear envelope, as well as lamin A\/C-PCAF interaction, are reduced by transient expression of lamin A mutated forms causing Emery Dreifuss muscular dystrophy. Consistent with this observation, lamin A\/C interaction with both PCAF and HDAC2 is significantly reduced in Emery\u2013Dreifuss muscular dystrophy myoblasts. Overall, these results support the view that, by recruiting PCAF and HDAC2 in a molecular platform, lamin A\/C might contribute to regulate their epigenetic activity required in the early phase of muscle differentiation.<\/jats:p>","DOI":"10.3390\/cells9071735","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T06:38:55Z","timestamp":1595313535000},"page":"1735","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["PCAF Involvement in Lamin A\/C-HDAC2 Interplay during the Early Phase of Muscle Differentiation"],"prefix":"10.3390","volume":"9","author":[{"given":"Spartaco","family":"Santi","sequence":"first","affiliation":[{"name":"Unit of Bologna, CNR Institute of Molecular Genetics \u201cLuigi Luca Cavalli Sforza\u201d, 40136 Bologna, Italy"},{"name":"IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-1840","authenticated-orcid":false,"given":"Vittoria","family":"Cenni","sequence":"additional","affiliation":[{"name":"Unit of Bologna, CNR Institute of Molecular Genetics \u201cLuigi Luca Cavalli Sforza\u201d, 40136 Bologna, Italy"},{"name":"IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Capanni","sequence":"additional","affiliation":[{"name":"Unit of Bologna, CNR Institute of Molecular Genetics \u201cLuigi Luca Cavalli Sforza\u201d, 40136 Bologna, Italy"},{"name":"IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7103-8722","authenticated-orcid":false,"given":"Giovanna","family":"Lattanzi","sequence":"additional","affiliation":[{"name":"Unit of Bologna, CNR Institute of Molecular Genetics \u201cLuigi Luca Cavalli Sforza\u201d, 40136 Bologna, Italy"},{"name":"IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1395-1927","authenticated-orcid":false,"given":"Elisabetta","family":"Mattioli","sequence":"additional","affiliation":[{"name":"Unit of Bologna, CNR Institute of Molecular Genetics \u201cLuigi Luca Cavalli Sforza\u201d, 40136 Bologna, Italy"},{"name":"IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.bbagrm.2015.01.002","article-title":"Regulation of skeletal muscle development and homeostasis by gene imprinting, histone acetylation and microRNA","volume":"1894","author":"Moresi","year":"2015","journal-title":"Biochim. 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