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The aim of this study was to investigate IL-13 as a therapeutic target during short term and chronic intrahepatic cholestasis in an Abcb4-knockout mouse model (Abcb4\u2212\/\u2212). Lack of IL-13 protected Abcb4\u2212\/\u2212 mice transiently from cholestasis. This decrease in serum bile acids was accompanied by an enhanced excretion of bile acids and a normalization of fecal bile acid composition. In Abcb4\u2212\/\u2212\/IL-13\u2212\/\u2212 double knockout mice, bacterial translocation to the liver was significantly reduced and the intestinal microbiome resembled the commensal composition in wild type animals. In addition, 52-week-old Abcb4\u2212\/\u2212IL-13\u2212\/\u2212 mice showed significantly reduced hepatic fibrosis. Abcb4\u2212\/\u2212 mice devoid of IL-13 transiently improved cholestasis and converted the composition of the gut microbiota towards healthy conditions. This highlights IL-13 as a potential therapeutic target in biliary diseases.<\/jats:p>","DOI":"10.3390\/cells9091949","type":"journal-article","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T10:37:32Z","timestamp":1598265452000},"page":"1949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["IL-13 as Target to Reduce Cholestasis and Dysbiosis in Abcb4 Knockout Mice"],"prefix":"10.3390","volume":"9","author":[{"given":"Luisa","family":"Hahn","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Justus-Liebig-University, D-35392 Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Helmrich","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Justus-Liebig-University, D-35392 Giessen, 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