{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:44:13Z","timestamp":1785815053173,"version":"3.56.0"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T00:00:00Z","timestamp":1601856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2018\/05181-7"],"award-info":[{"award-number":["#2018\/05181-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Regulatory B (B10) cells can control several inflammatory diseases, including allergies; however, the origin of peripheral B10 cells is not fully understood, and the involvement of primary lymphoid organs (PLOs) as a primary site of maturation is not known. Here, using a murine model of allergy inhibition mediated by maternal immunization with ovalbumin (OVA), we aimed to evaluate whether B10 cells can mature in the thymus and whether IgG can mediate this process. Female mice were immunized with OVA, and offspring thymus, bone marrow, spleen, lung, and serum samples were evaluated at different times and after passive transfer of purified IgG or thymocytes. A translational approach was implemented using human nonatopic thymus samples, nonatopic peripheral blood mononuclear cells (PBMCs), and IgG from atopic or nonatopic individuals. Based on the expression of CD1d on B cells during maturation stages, we suggest that B10 cells can also mature in the murine thymus. Murine thymic B10 cells can be induced in vitro and in vivo by IgG and be detected in the spleen and lungs in response to an allergen challenge. Like IgG from atopic individuals, human IgG from nonatopic individuals can induce B10 cells in the infant thymus and adult PBMCs. Our observations suggest that B10 cells may mature in the thymus and that this mechanism may be mediated by IgG in both humans and mice. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies.<\/jats:p>","DOI":"10.3390\/cells9102239","type":"journal-article","created":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T08:35:57Z","timestamp":1601886957000},"page":"2239","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The Potential of IgG to Induce Murine and Human Thymic Maturation of IL-10+ B Cells (B10) Revealed in a Pilot Study"],"prefix":"10.3390","volume":"9","author":[{"given":"Amanda Harumi Sab\u00f4","family":"Inoue","sequence":"first","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9405-4955","authenticated-orcid":false,"given":"Aline Aparecida de Lima","family":"Lira","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia Garcia","family":"de-Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamires Rodrigues","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio da Ressurei\u00e7\u00e3o","family":"Sgnotto","sequence":"additional","affiliation":[{"name":"Division of Hematology, Medical School, University of Sao Paulo, Sao Paulo 01246-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto Jos\u00e9 da Silva","family":"Duarte","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"},{"name":"Division of Pathology, Medical School, University of Sao Paulo, Sao Paulo 01246-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6092-8394","authenticated-orcid":false,"given":"Jefferson Russo","family":"Victor","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil"},{"name":"Division of Environmental Health, FMU, Laureate International Universities, Sao Paulo 04505-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.2353\/ajpath.2006.051231","article-title":"Adoptively transferred allergen-specific T cells cause maternal transmission of asthma risk","volume":"168","author":"Hubeau","year":"2006","journal-title":"Am. 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