{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:02:27Z","timestamp":1788307347984,"version":"build-2803163510"},"reference-count":66,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"William Stamps Farish Fund","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY012857"],"award-info":[{"award-number":["R01EY012857"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["T35NS064931"],"award-info":[{"award-number":["T35NS064931"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>More than 1.5 million people suffer from Retinitis Pigmentosa, with many experiencing partial to complete vision loss. Regenerative therapies offer some hope, but their development is challenged by the limited regenerative capacity of mammalian model systems. As a step toward investigating regenerative therapies, we developed a zebrafish model of Retinitis Pigmentosa that displays ongoing regeneration. We used Tol2 transgenesis to express mouse rhodopsin carrying the P23H mutation and an epitope tag in zebrafish rod photoreceptors. Adult and juvenile fish were examined by immunofluorescence, TUNEL and BrdU incorporation assays. P23H transgenic fish expressed the transgene in rods from 3 days post fertilization onward. Rods expressing the mutant rhodopsin formed very small or no outer segments and the mutant protein was delocalized over the entire cell. Adult fish displayed thinning of the outer nuclear layer (ONL) and loss of rod outer segments, but retained a single, sparse row of rods. Adult fish displayed ongoing apoptotic cell death in the ONL and an abundance of proliferating cells, predominantly in the ONL. There was a modest remodeling of bipolar and M\u00fcller glial cells. This transgenic fish will provide a useful model system to study rod photoreceptor regeneration and integration.<\/jats:p>","DOI":"10.3390\/cells9102242","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T10:46:17Z","timestamp":1601981177000},"page":"2242","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["A Zebrafish Model of Retinitis Pigmentosa Shows Continuous Degeneration and Regeneration of Rod Photoreceptors"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1952-431X","authenticated-orcid":false,"given":"Abirami","family":"Santhanam","sequence":"first","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3140-1523","authenticated-orcid":false,"given":"Eyad","family":"Shihabeddin","sequence":"additional","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"The MD Anderson Cancer Center\/UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua A.","family":"Atkinson","sequence":"additional","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ping","family":"Lin","sequence":"additional","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0270-3442","authenticated-orcid":false,"given":"John","family":"O\u2019Brien","sequence":"additional","affiliation":[{"name":"Ruiz Department of Ophthalmology &amp; Visual Science, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"The MD Anderson Cancer Center\/UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4526","DOI":"10.1073\/pnas.93.10.4526","article-title":"Retinitis pigmentosa: Unfolding its mystery","volume":"93","author":"Berson","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1016\/S0140-6736(06)69740-7","article-title":"Retinitis pigmentosa","volume":"368","author":"Hartong","year":"2006","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1001\/archophthalmol.2011.2820","article-title":"Health services utilization and cost of retinitis pigmentosa","volume":"130","author":"Frick","year":"2012","journal-title":"Arch. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1001\/archopht.125.2.151","article-title":"Perspective on genes and mutations causing retinitis pigmentosa","volume":"125","author":"Daiger","year":"2007","journal-title":"Arch. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1093\/hmg\/11.10.1219","article-title":"Retinitis pigmentosa and allied diseases: Numerous diseases, genes, and inheritance patterns","volume":"11","author":"Rivolta","year":"2002","journal-title":"Hum. Mol. Genet."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Daiger, S.P., Bowne, S.J., and Sullivan, L.S. (2015). Genes and mutations causing autosomal dominant retinitis pigmentosa. Cold Spring Harb. Perspect. Med., 5.","DOI":"10.1101\/cshperspect.a017129"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1136\/jmg.2005.031682","article-title":"Molecular genetics of autosomal dominant retinitis pigmentosa (ADRP): A comprehensive study of 43 Italian families","volume":"42","author":"Ziviello","year":"2005","journal-title":"J. Med. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3052","DOI":"10.1167\/iovs.05-1443","article-title":"Prevalence of disease-causing mutations in families with autosomal dominant retinitis pigmentosa","volume":"47","author":"Sullivan","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9288","DOI":"10.1074\/jbc.M114.551713","article-title":"Inherent instability of the retinitis pigmentosa P23H mutant opsin","volume":"289","author":"Chen","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6121","DOI":"10.1021\/bi00186a011","article-title":"Structure and function in rhodopsin. 7. Point mutations associated with autosomal dominant retinitis pigmentosa","volume":"33","author":"Kaushal","year":"1994","journal-title":"Biochemistry"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"14442","DOI":"10.1074\/jbc.M300087200","article-title":"Pharmacological chaperone-mediated in vivo folding and stabilization of the P23H-opsin mutant associated with autosomal dominant retinitis pigmentosa","volume":"278","author":"Noorwez","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/S0042-6989(01)00146-8","article-title":"Retinal degeneration mutants in the mouse","volume":"42","author":"Chang","year":"2002","journal-title":"Vis. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1167\/iovs.05-0845","article-title":"A frameshift mutation in RPGR exon ORF15 causes photoreceptor degeneration and inner retina remodeling in a model of X-linked retinitis pigmentosa","volume":"47","author":"Beltran","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Falasconi, A., Biagioni, M., Novelli, E., Piano, I., Gargini, C., and Strettoi, E. (2019). Retinal phenotype in the rd9 mutant mouse, a model of X-linked RP. Front. Neurosci., 13.","DOI":"10.3389\/fnins.2019.00991"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/0896-6273(92)90236-7","article-title":"Transgenic mice with a rhodopsin mutation (Pro23His): A mouse model of autosomal dominant retinitis pigmentosa","volume":"9","author":"Olsson","year":"1992","journal-title":"Neuron"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5499","DOI":"10.1073\/pnas.90.12.5499","article-title":"Simulation of human autosomal dominant retinitis pigmentosa in transgenic mice expressing a mutated murine opsin gene","volume":"90","author":"Naash","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1038\/nm0898-967","article-title":"Ribozyme rescue of photoreceptor cells in a transgenic rat model of autosomal dominant retinitis pigmentosa","volume":"4","author":"Lewin","year":"1998","journal-title":"Nat. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10551","DOI":"10.1074\/jbc.M110.209759","article-title":"Probing mechanisms of photoreceptor degeneration in a new mouse model of the common form of autosomal dominant retinitis pigmentosa due to P23H opsin mutations","volume":"286","author":"Sakami","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.gde.2016.05.028","article-title":"Retinal regeneration in birds and mice","volume":"40","author":"Wilken","year":"2016","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.gde.2016.05.009","article-title":"Retina regeneration in zebrafish","volume":"40","author":"Wan","year":"2016","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/j.celrep.2019.04.047","article-title":"The hippo pathway blocks mammalian retinal m\u00fcller glial cell reprogramming","volume":"27","author":"Rueda","year":"2019","journal-title":"Cell Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/dvdy.24375","article-title":"M\u00fcller glial cell-dependent regeneration of the neural retina: An overview across vertebrate model systems","volume":"245","author":"Hamon","year":"2016","journal-title":"Dev. Dyn."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/nrn3723","article-title":"M\u00fcller glial cell reprogramming and retina regeneration","volume":"15","author":"Goldman","year":"2014","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0959-437X(00)00074-5","article-title":"Zebrafish: A model system for the study of human disease","volume":"10","author":"Dooley","year":"2000","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3390","DOI":"10.1523\/JNEUROSCI.10-10-03390.1990","article-title":"Distribution of photoreceptor subtypes in the retina of diurnal and nocturnal primates","volume":"10","author":"Wikler","year":"1990","journal-title":"J. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/S0736-5748(01)00050-8","article-title":"The zebrafish as a model visual system","volume":"19","author":"Bilotta","year":"2001","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.neuron.2020.04.021","article-title":"Fovea-like photoreceptor specializations underlie single UV cone driven prey-capture behavior in Zebrafish","volume":"107","author":"Yoshimatsu","year":"2020","journal-title":"Neuron"},{"key":"ref_28","unstructured":"Westerfield, M. (2000). The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio), University of Oregon Press. [4th ed.]. Available online: https:\/\/zfin.org\/zf_info\/zfbook\/cont.html."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1534\/genetics.106.060244","article-title":"Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition","volume":"174","author":"Urasaki","year":"2006","journal-title":"Genetics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.devcel.2004.06.005","article-title":"A transposon-mediated gene trap approach identifies developmentally regulated genes in Zebrafish","volume":"7","author":"Kawakami","year":"2004","journal-title":"Dev. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"610","DOI":"10.2144\/000112619","article-title":"Method for isolation of PCR-ready genomic DNA from zebrafish tissues","volume":"43","author":"Meeker","year":"2007","journal-title":"BioTechniques"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/00019606-200603000-00009","article-title":"Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts","volume":"15","author":"Arocho","year":"2006","journal-title":"Diagn. Mol. Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1242\/dev.109.3.567","article-title":"Early onset of phenotype and cell patterning in the embryonic zebrafish retina","volume":"109","author":"Larison","year":"1990","journal-title":"Development"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Nakao, T., Tsujikawa, M., Notomi, S., Ikeda, Y., and Nishida, K. (2012). The Role of mislocalized phototransduction in photoreceptor cell death of retinitis pigmentosa. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0032472"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/0014-4835(86)90030-8","article-title":"Rhodopsin\u2019s amino terminus is a principal antigenic site","volume":"42","author":"Hargrave","year":"1986","journal-title":"Exp. Eye Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4762","DOI":"10.1167\/iovs.05-0797","article-title":"Cone Survival Despite Rod Degeneration in XOPS-mCFP Transgenic Zebrafish","volume":"46","author":"Morris","year":"2005","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0012-1606(03)00125-8","article-title":"Development of a rod photoreceptor mosaic revealed in transgenic zebrafish","volume":"258","author":"Fadool","year":"2003","journal-title":"Dev. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rashid, K., Akhtar-Schaefer, I., and Langmann, T. (2019). Microglia in retinal degeneration. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.01975"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"33356","DOI":"10.1038\/srep33356","article-title":"Persistent inflammatory state after photoreceptor loss in an animal model of retinal degeneration","volume":"6","author":"Noailles","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1177\/40.9.1354677","article-title":"Detection of proliferating cell nuclear antigen in diagnostic histopathology","volume":"40","author":"Wolf","year":"1992","journal-title":"J. Histochem. Cytochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.stemcr.2017.02.007","article-title":"Neurotransmitter-regulated regeneration in the Zebrafish retina","volume":"8","author":"Rao","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1523\/JNEUROSCI.5317-06.2007","article-title":"Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish","volume":"27","author":"Fimbel","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0014-4827(73)90255-3","article-title":"Evaluation of S phase synchronization by analysis of DNA replication in 5-bromodeoxyuridine","volume":"82","author":"Meyn","year":"1973","journal-title":"Exp. Cell Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s00418-018-1764-8","article-title":"Differential expression of PKC\u03b1 and -\u03b2 in the zebrafish retina","volume":"151","author":"Haug","year":"2019","journal-title":"Histochem. Cell Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.preteyeres.2009.07.001","article-title":"Cellular signaling and factors involved in M\u00fcller cell gliosis: Neuroprotective and detrimental effects","volume":"28","author":"Bringmann","year":"2009","journal-title":"Progress Retin. Eye Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"11020","DOI":"10.1073\/pnas.190291097","article-title":"Modifications of retinal neurons in a mouse model of retinitis pigmentosa","volume":"97","author":"Strettoi","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1089\/hum.2012.067","article-title":"Proinsulin slows retinal degeneration and vision loss in the P23H rat model of retinitis pigmentosa","volume":"23","author":"Lax","year":"2012","journal-title":"Hum. Gene Ther."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.neuint.2008.10.014","article-title":"Role of retinal glial cells in neurotransmitter uptake and metabolism","volume":"54","author":"Bringmann","year":"2009","journal-title":"Neurochem. Int."},{"key":"ref_49","first-page":"1197","article-title":"Gene expression changes within M\u00fcller glial cells in retinitis pigmentosa","volume":"18","author":"Roesch","year":"2012","journal-title":"Mol. Vis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00018-010-0563-8","article-title":"Genetics of photoreceptor degeneration and regeneration in zebrafish","volume":"68","author":"Brockerhoff","year":"2011","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10545","DOI":"10.1073\/pnas.92.23.10545","article-title":"A behavioral screen for isolating zebrafish mutants with visual system defects","volume":"92","author":"Brockerhoff","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"13866","DOI":"10.1523\/JNEUROSCI.3136-07.2007","article-title":"A Mutation in the cone-specific pde6 gene causes rapid cone photoreceptor degeneration in Zebrafish","volume":"27","author":"Stearns","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"19581","DOI":"10.1073\/pnas.0907720106","article-title":"A homologous genetic basis of the murine cpfl1 mutant and human achromatopsia linked to mutations in the PDE6C gene","volume":"106","author":"Chang","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0896-6273(04)00268-5","article-title":"Intraflagellar transport genes are essential for differentiation and survival of vertebrate sensory neurons","volume":"42","author":"Tsujikawa","year":"2004","journal-title":"Neuron"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4369","DOI":"10.1242\/dev.125.22.4369","article-title":"not really finished is crucial for development of the zebrafish outer retina and encodes a transcription factor highly homologous to human nuclear respiratory factor-1 and avian initiation binding repressor","volume":"125","author":"Becker","year":"1998","journal-title":"Development"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/ncb1706","article-title":"Elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8","volume":"10","author":"Omori","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1534\/genetics.104.039727","article-title":"Identification of zebrafish insertional mutants with defects in visual system development and function","volume":"170","author":"Gross","year":"2005","journal-title":"Genetics"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1523\/JNEUROSCI.20-05-01893.2000","article-title":"Effects of Dopamine Depletion on Visual Sensitivity of Zebrafish","volume":"20","author":"Li","year":"2000","journal-title":"J. Neurosci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.mad.2005.03.025","article-title":"Behavioral screening for nightblindness mutants in zebrafish reveals three new loci that cause dominant photoreceptor cell degeneration","volume":"126","author":"Maaswinkel","year":"2005","journal-title":"Mech. Ageing Dev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1002\/mrd.20831","article-title":"Zebrafish mutants as models for congenital ocular disorders in humans","volume":"75","author":"Gross","year":"2008","journal-title":"Mol. Reprod. Dev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6225","DOI":"10.1074\/jbc.M116.760314","article-title":"Pathogenic mutations in retinitis pigmentosa 2 predominantly result in loss of RP2 protein stability in humans and zebrafish","volume":"292","author":"Liu","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1167\/iovs.10-6800","article-title":"Knockdown of the Zebrafish ortholog of the retinitis pigmentosa 2 (RP2) gene results in retinal degeneration","volume":"52","author":"Shu","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/dneu.20362","article-title":"Time course analysis of gene expression during light-induced photoreceptor cell death and regeneration in albino zebrafish","volume":"67","author":"Kassen","year":"2007","journal-title":"Dev. Neurobiol."},{"key":"ref_64","first-page":"775","article-title":"Gene expression profiles of intact and regenerating zebrafish retina","volume":"11","author":"Cameron","year":"2005","journal-title":"Mol. Vis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"7028","DOI":"10.1523\/JNEUROSCI.1624-07.2007","article-title":"Late-Stage neuronal progenitors in the retina are radial m\u00fcller glia that function as retinal stem cells","volume":"27","author":"Bernardos","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1002\/dneu.20610","article-title":"Genetic dissection reveals two separate pathways for rod and cone regeneration in the teleost retina","volume":"68","author":"Morris","year":"2008","journal-title":"Dev. Neurobiol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/10\/2242\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:16:51Z","timestamp":1760177811000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/10\/2242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,6]]},"references-count":66,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["cells9102242"],"URL":"https:\/\/doi.org\/10.3390\/cells9102242","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,6]]}}}