{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:30:58Z","timestamp":1778283058166,"version":"3.51.4"},"reference-count":70,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["U24 NS072026"],"award-info":[{"award-number":["U24 NS072026"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30 AD19610"],"award-info":[{"award-number":["P30 AD19610"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R21AG056706"],"award-info":[{"award-number":["R21AG056706"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007306","name":"Arizona Department of Health Services","doi-asserted-by":"publisher","award":["211002"],"award-info":[{"award-number":["211002"]}],"id":[{"id":"10.13039\/100007306","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008335","name":"Arizona Biomedical Research Commission","doi-asserted-by":"publisher","award":["4001, 0011, 05-901, 1001"],"award-info":[{"award-number":["4001, 0011, 05-901, 1001"]}],"id":[{"id":"10.13039\/100008335","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>The Arizona Study of Aging and Neurodegenerative Disorders\/Brain and Body Donation Program at Banner Sun Health Research Institute (BSHRI) is a longitudinal clinicopathological study with a current enrollment of more than 900 living subjects for aging and neurodegenerative disease research. Annual clinical assessments are done by cognitive and movement neurologists and neuropsychologists. Brain and body tissues are collected at a median postmortem interval of 3.0 h for neuropathological diagnosis and banking. Since 2018, the program has undertaken banking of scalp fibroblasts derived from neuropathologically characterized donors with Alzheimer\u2019s disease, Parkinson\u2019s disease, and other neurodegenerative diseases. Here, we describe the procedure development and cell characteristics from 14 male and 15 female donors (mean \u00b1 SD of age: 83.6 \u00b1 12.2). Fibroblasts from explant cultures were banked at passage 3. The results of mRNA analysis showed positive expression of fibroblast activation protein, vimentin, fibronectin, and THY1 cell surface antigen. We also demonstrated that the banked fibroblasts from a postmortem elderly donor were successfully reprogramed to human-induced pluripotent stem cells (hiPSCs). Taken together, we have demonstrated the successful establishment of a human autopsy-derived fibroblast banking program. The cryogenically preserved cells are available for request at the program website of the BSHRI.<\/jats:p>","DOI":"10.3390\/cells9112383","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T09:29:32Z","timestamp":1604050172000},"page":"2383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research"],"prefix":"10.3390","volume":"9","author":[{"given":"Suet Theng","family":"Beh","sequence":"first","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlye","family":"Frisch","sequence":"additional","affiliation":[{"name":"School of Biological and Health Systems Engineering, Arizona State University, 501 E. Tyler Mall, ECG 334A, Tempe, AZ 85287, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Brafman","sequence":"additional","affiliation":[{"name":"School of Biological and Health Systems Engineering, Arizona State University, 501 E. Tyler Mall, ECG 334A, Tempe, AZ 85287, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared","family":"Churko","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85721, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica E.","family":"Walker","sequence":"additional","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geidy E.","family":"Serrano","sequence":"additional","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia I.","family":"Sue","sequence":"additional","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric M.","family":"Reiman","sequence":"additional","affiliation":[{"name":"Banner Alzheimer\u2019s Institute, Phoenix, AZ 85006, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas G.","family":"Beach","sequence":"additional","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lih-Fen","family":"Lue","sequence":"additional","affiliation":[{"name":"Banner Sun Health Research Institute, Sun City, AZ 85351, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1517\/17460441.2015.1041913","article-title":"Rodent models for Alzheimer\u2019s disease drug discovery","volume":"10","author":"Puzzo","year":"2015","journal-title":"Expert Opin Drug Discov."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1038\/s41593-018-0236-8","article-title":"Animal models of neurodegenerative diseases","volume":"21","author":"Dawson","year":"2018","journal-title":"Nat. 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