{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:51:10Z","timestamp":1783047070916,"version":"3.54.6"},"reference-count":135,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T00:00:00Z","timestamp":1604275200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["675671"],"award-info":[{"award-number":["675671"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Enterococci are the second most common Gram-positive pathogen responsible for nosocomial infections. Due to the limited number of new antibiotics that reach the medical practice and the resistance of enterococci to the current antibiotic options, passive and active immunotherapies have emerged as a potential prevention and\/or treatment strategy against this opportunistic pathogen. In this review, we explore the pathogenicity of these bacteria and their interaction with the host immune response. We provide an overview of the capsular polysaccharides and surface-associated proteins that have been described as potential antigens in anti-enterococcal vaccine formulations. In addition, we describe the current status in vaccine development against enterococci and address the importance and the current advances toward the development of well-defined vaccines with broad coverage against enterococci.<\/jats:p>","DOI":"10.3390\/cells9112397","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T09:04:46Z","timestamp":1604307886000},"page":"2397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Advances and Prospects in Vaccine Development against Enterococci"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4125-5101","authenticated-orcid":false,"given":"Ermioni","family":"Kalfopoulou","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Huebner","sequence":"additional","affiliation":[{"name":"Division of Paediatric Infectious Diseases, Dr. von Hauner Children\u2019s Hospital, Ludwig Maximilians University, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/nrmicro2761","article-title":"The rise of the Enterococcus: Beyond vancomycin resistance","volume":"10","author":"Arias","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1089\/mdr.2016.0320","article-title":"Virulence and Antibiotic Resistance of Enterococci Isolated from Healthy Breastfed Infants","volume":"24","author":"Landete","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2005.06.026","article-title":"The role and application of enterococci in food and health","volume":"106","author":"Sarantinopoulos","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_4","first-page":"5938210","article-title":"Enterococci: Between Emerging Pathogens and Potential Probiotics","volume":"2019","author":"Smaoui","year":"2019","journal-title":"Biomed Res. Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/CMR.7.4.462","article-title":"Virulence of enterococci","volume":"7","author":"Jett","year":"1994","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3201\/eid0402.980211","article-title":"Multiple-Drug Resistant Enterococci: The Nature of the Problem and an Agenda for the Future","volume":"4","author":"Huycke","year":"1998","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4161\/viru.21282","article-title":"Intrinsic and acquired resistance mechanisms in enterococcus","volume":"3","author":"Hollenbeck","year":"2012","journal-title":"Virulence"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41579-018-0107-z","article-title":"Biofilm-associated infection by enterococci","volume":"17","author":"Chong","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/j.vaccine.2003.11.027","article-title":"Enterococcal infections: Host response, therapeutic, and prophylactic possibilities","volume":"22","author":"Koch","year":"2004","journal-title":"Vaccine"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1084\/jem.4.5-6.521","article-title":"A case of acute endocarditis cause by Micrococcous zymogenes (NOV. SPEC.), with a description of the microorganism","volume":"4","author":"MacCallum","year":"1899","journal-title":"J. Exp. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1080\/21505594.2017.1279766","article-title":"Model systems for the study of Enterococcal colonization and infection","volume":"8","author":"Goh","year":"2017","journal-title":"Virulence"},{"key":"ref_12","unstructured":"Cassini, A., H\u00f6gberg, L.D., Plachouras, D., Quattrocchi, A., Hoxha, A., Simonsen, G.S., Colomb-Cotinat, M., Kretzschmar, M.E., Devleesschauwer, B., and Cecchini, M. (2018). Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: A population-level modelling analysis. Lancet Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"19046","DOI":"10.2807\/ese.13.47.19046-en","article-title":"Emergence and spread of vancomycin resistance among enterococci in Europe","volume":"13","author":"Werner","year":"2008","journal-title":"Eur. Surveill."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Barros, C.H.N., Fulaz, S., Stanisic, D., and Tasic, L. (2018). Biogenic Nanosilver against Multidrug-Resistant Bacteria (MDRB). Antibiotics, 7.","DOI":"10.3390\/antibiotics7030069"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1126\/science.1110591","article-title":"Diversity of the human intestinal microbial flora","volume":"308","author":"Eckburg","year":"2005","journal-title":"Science"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/nature07250","article-title":"Vancomycin-resistant enterococci exploit antibiotic-induced innate immune deficits","volume":"455","author":"Brandl","year":"2008","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1086\/650203","article-title":"Bacterial flagellin stimulates Toll-like receptor 5-dependent defense against vancomycin-resistant Enterococcus infection","volume":"201","author":"Kinnebrew","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4332","DOI":"10.1172\/JCI43918","article-title":"Vancomycin-resistant Enterococcus domination of intestinal microbiota is enabled by antibiotic treatment in mice and precedes bloodstream invasion in humans","volume":"120","author":"Ubeda","year":"2010","journal-title":"J. Clin. Investig."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Singh, K.V., Nallapareddy, S.R., Sillanp\u00e4\u00e4, J., and Murray, B.E. (2010). Importance of the Collagen Adhesin Ace in Pathogenesis and Protection against Enterococcus faecalis Experimental Endocarditis. PLoS Pathog., 6.","DOI":"10.1371\/annotation\/1ccae8f8-d274-4ff8-a295-815037ce9cc6"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.juro.2016.01.100","article-title":"Fibrinogen Release and Deposition on Urinary Catheters Placed during Urological Procedures","volume":"196","author":"Walker","year":"2016","journal-title":"J. Urol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Flores-Mireles, A.L., Pinkner, J.S., Caparon, M.G., and Hultgren, S.J. (2014). EbpA vaccine antibodies block binding of Enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice. Sci. Transl. Med., 6.","DOI":"10.1126\/scitranslmed.3009384"},{"key":"ref_22","unstructured":"Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., and Walter, P. (2002). Molecular Biology of the Cell, Garland Science. [4th ed.]. ISBN-10: 0-8153-3218-1."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"17406","DOI":"10.1074\/jbc.274.25.17406","article-title":"Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2","volume":"274","author":"Schwandner","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/IAI.00863-08","article-title":"Neutrophils Are Essential for Rapid Clearance of Enterococcus faecium in Mice","volume":"77","author":"Leendertse","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/1753425908100238","article-title":"Peritoneal macrophages are important for the early containment of Enterococcus faecium peritonitis in mice","volume":"15","author":"Leendertse","year":"2009","journal-title":"Innate Immun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1086\/650341","article-title":"The Complement System Facilitates Clearance of Enterococcus faecium during Murine Peritonitis","volume":"201","author":"Leendertse","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/nrmicro2128","article-title":"Antimicrobial mechanisms of phagocytes and bacterial evasion strategies","volume":"7","author":"Flannagan","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_28","first-page":"467","article-title":"Neutrophil-Mediated Phagocytosis of Staphylococcus aureus","volume":"5","author":"Bestebroer","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0168-1605(03)00189-2","article-title":"Opsonophagocytic assay as a potentially useful tool for assessing safety of enterococcal preparations","volume":"88","author":"Hufnagel","year":"2003","journal-title":"Int. J. Food Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1128\/iai.62.3.987-993.1994","article-title":"Roles of antibodies and complement in phagocytic killing of enterococci","volume":"62","author":"Arduino","year":"1994","journal-title":"Infect. Immun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1128\/IAI.67.3.1213-1219.1999","article-title":"Isolation and chemical characterization of a capsular polysaccharide antigen shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium","volume":"67","author":"Huebner","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s15010-005-5063-7","article-title":"In vitro Assessment of the Host Response against Enterococcus faecalis Used in Probiotic Preparations","volume":"33","author":"Kropec","year":"2005","journal-title":"Infection"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Romero-Saavedra, F., Laverde, D., Wobser, D., Michaux, C., Budin-Verneuil, A., Bernay, B., Benachour, A., Hartke, A., and Huebner, J. (2014). Identification of Peptidoglycan-Associated Proteins as Vaccine Candidates for Enterococcal Infections. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111880"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5587","DOI":"10.1128\/iai.62.12.5587-5594.1994","article-title":"Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis","volume":"62","author":"Arduino","year":"1994","journal-title":"Infect. Immun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6067","DOI":"10.1128\/IAI.67.11.6067-6075.1999","article-title":"Enterococcus faecalis bearing aggregation substance is resistant to killing by human neutrophils despite phagocytosis and neutrophil activation","volume":"67","author":"Rakita","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s00430-001-0096-8","article-title":"Enterococcus spp. produces slime and survives in rat peritoneal macrophages","volume":"190","author":"Baldassarri","year":"2001","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2160","DOI":"10.1128\/IAI.67.5.2160-2165.1999","article-title":"Survival of Enterococcus faecalis in mouse peritoneal macrophages","volume":"67","author":"Pikis","year":"1999","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1056\/NEJMoa025025","article-title":"Infection with Vancomycin-Resistant Staphylococcus aureus Containing the vanA Resistance Gene","volume":"348","author":"Chang","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.3389\/fmicb.2015.01534","article-title":"Enterococcus faecalis from food, clinical specimens, and oral sites: Prevalence of virulence factors in association with biofilm formation","volume":"6","author":"Anderson","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"18574","DOI":"10.1074\/jbc.RA118.006052","article-title":"The structures of penicillin-binding protein 4 (PBP4) and PBP5 from Enterococci provide structural insights into \u03b2-lactam resistance","volume":"293","author":"Moon","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1172\/JCI106758","article-title":"Studies on antibiotic syngerism against enterococci. II. Effect of various antibiotics on the uptake of 14 C-labeled streptomycin by enterococci","volume":"50","author":"Moellering","year":"1971","journal-title":"J. Clin. Investig."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1086\/313949","article-title":"Aminoglycoside Resistance in Enterococci","volume":"31","author":"Chow","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1089\/mdr.1999.5.159","article-title":"First Confirmed Case of a Vancomycin-Resistant Enterococcus faecium with vanA Phenotype from Brazil: Isolation from a Meningitis Case in S\u00e3o Paulo","volume":"5","author":"Zanella","year":"1999","journal-title":"Microb. Drug Resist."},{"key":"ref_44","unstructured":"Kristich, C.J., Rice, L.B., and Arias, C.A. (2014). Enterococci: From Commensals to Leading Causes of Drug Resistant Infection, Massachusetts Eye and Ear Infirmary. Enterococcal Infection\u2014Treatment and Antibiotic Resistance."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1046\/j.1365-2958.1996.00617.x","article-title":"Quantitative analysis of the metabolism of soluble cytoplasmic peptidoglycan precursors of glycopeptide-resistant enterococci","volume":"21","author":"Arthur","year":"1996","journal-title":"Mol. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Iseppi, R., Di Cerbo, A., Messi, P., and Sabia, C. (2020). Antibiotic resistance and virulence traits in vancomycin-resistant enterococci (Vre) and extended-spectrum \u03b2-lactamase\/ampc-producing (ESBL\/ampc) enterobacteriaceae from humans and pets. Antibiotics, 9.","DOI":"10.3390\/antibiotics9040152"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2119","DOI":"10.1128\/AAC.37.10.2119","article-title":"Identification of the satA gene encoding a streptogramin A acetyltransferase in Enterococcus faecium BM4145","volume":"37","author":"Leclercq","year":"1993","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1128\/AAC.43.7.1813","article-title":"Characterization of a new enterococcal gene, satG, encoding a putative acetyltransferase conferring resistance to Streptogramin A compounds","volume":"43","author":"Werner","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1128\/AAC.44.4.967-971.2000","article-title":"Macrolide resistance genes in Enterococcus spp.","volume":"44","author":"Portillo","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1128\/AAC.46.6.1845-1850.2002","article-title":"An Enterococcus faecalis ABC homologue (Lsa) is required for the resistance of this species to clindamycin and quinupristin-dalfopristin","volume":"46","author":"Singh","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1345\/aph.1D377","article-title":"Treatment of Vancomycin-Resistant Enterococcus with Quinupristin\/Dalfopristin and High-Dose Ampicillin","volume":"38","author":"Bethea","year":"2004","journal-title":"Ann. Pharmacother."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1086\/517755","article-title":"Treatment of Endocarditis Due to Vancomycin-Resistant Enterococcus faecium with Quinupristin\/Dalfopristin, Doxycycline, and Rifampin: A Synergistic Drug Combination","volume":"27","author":"Matsumura","year":"1998","journal-title":"Clin. Infect. Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1056\/NEJMoa1011138","article-title":"Genetic Basis for In Vivo Daptomycin Resistance in Enterococci","volume":"365","author":"Arias","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s11908-012-0270-8","article-title":"Enterococcal endocarditis: Can we win the war?","volume":"14","author":"Munita","year":"2012","journal-title":"Curr. Infect. Dis. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1586\/14787210.6.5.637","article-title":"Emergence and management of drug-resistant enterococcal infections","volume":"6","author":"Arias","year":"2008","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3334","DOI":"10.1128\/AAC.46.10.3334-3336.2002","article-title":"Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis","volume":"46","author":"Marshall","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1128\/AAC.06091-11","article-title":"First Report of the Multidrug Resistance Gene cfr in Enterococcus faecalis of Animal Origin","volume":"56","author":"Liu","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1084\/jem.57.4.571","article-title":"A serological differentiation of human and other groups of hemolytic streptococci","volume":"57","author":"Lancefield","year":"1933","journal-title":"J. Exp. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1111\/j.1348-0421.1992.tb02070.x","article-title":"Proposal of a new scheme for the serological typing of Enterococcus faecalis strains","volume":"36","author":"Maekawa","year":"1992","journal-title":"Microbiol. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1128\/JCM.42.6.2548-2557.2004","article-title":"Serological and Genetic Diversity of Capsular Polysaccharides in Enterococcus faecalis","volume":"42","author":"Hufnagel","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s15010-006-4100-5","article-title":"Distribution of Four Capsular Serotypes of Enterococcus faecalis among Clinical Isolates from Different Geographical Origins and Infection Sites","volume":"34","author":"Hufnagel","year":"2006","journal-title":"Infection"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1073\/pnas.032448299","article-title":"The capsular polysaccharide of Enterococcus faecalis and its relationship to other polysaccharides in the cell wall","volume":"99","author":"Hancock","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"6203","DOI":"10.1128\/JB.00592-09","article-title":"Capsular polysaccharide production in Enterococcus faecalis and contribution of CpsF to capsule serospecificity","volume":"191","author":"Thurlow","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5551","DOI":"10.1128\/IAI.00576-09","article-title":"Enterococcus faecalis capsular polysaccharide serotypes C and D and their contributions to host innate immune evasion","volume":"77","author":"Thurlow","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Theilacker, C., Kaczy\u0144ski, Z., Kropec, A., Sava, I., Ye, L., Bychowska, A., Holst, O., and Huebner, J. (2011). Serodiversity of Opsonic Antibodies against Enterococcus faecalis\u2014Glycans of the Cell Wall Revisited. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017839"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"McBride, S.M., Fischetti, V.A., LeBlanc, D.J., Moellering, R.C., and Gilmore, M.S. (2007). Genetic diversity among Enterococcus faecalis. PLoS ONE, 2.","DOI":"10.1371\/journal.pone.0000582"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1111\/j.1469-0691.2010.03214.x","article-title":"Management of multidrug-resistant enterococcal infections","volume":"16","author":"Arias","year":"2010","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1128\/MMBR.63.1.174-229.1999","article-title":"Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope","volume":"63","author":"Navarre","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_69","unstructured":"Hancock, L.E., Murray, B.E., and Sillanp\u00e4\u00e4, J. (2014). Enterococci: From Commensals to Leading Causes of Drug Resistant Infection, Massachusetts Eye and Ear Infirmary. Enterococcal Cell Wall Components and Structures."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1111\/j.1574-695X.2000.tb01489.x","article-title":"Specific antibody promotes opsonization and PMN-mediated killing of phagocytosis-resistant Enterococcus faecium","volume":"28","author":"Rakita","year":"2000","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4631","DOI":"10.1128\/IAI.68.8.4631-4636.2000","article-title":"Prophylactic and therapeutic efficacy of antibodies to a capsular polysaccharide shared among vancomycin-sensitive and -resistant enterococci","volume":"68","author":"Huebner","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0008-6215(99)00046-4","article-title":"Structure of an antigenic teichoic acid shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium","volume":"316","author":"Wang","year":"1999","journal-title":"Carbohydr. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"5703","DOI":"10.1128\/IAI.00570-06","article-title":"Opsonic antibodies to Enterococcus faecalis strain 12030 are directed against lipoteichoic acid","volume":"74","author":"Theilacker","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/cbic.200900557","article-title":"Wall teichoic acid function, biosynthesis, and inhibition","volume":"11","author":"Swoboda","year":"2010","journal-title":"Chembiochem"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1093\/infdis\/jis022","article-title":"Protection Against Staphylococcus aureus by Antibody to the Polyglycerolphosphate Backbone of Heterologous Lipoteichoic Acid","volume":"205","author":"Theilacker","year":"2012","journal-title":"J. Infect. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3759","DOI":"10.1128\/IAI.00149-09","article-title":"Further characterization of the epa gene cluster and epa polysaccharides of Enterococcus faecalis","volume":"77","author":"Teng","year":"2009","journal-title":"Infect. Immun."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"37769","DOI":"10.1074\/jbc.M112.358283","article-title":"Secondary cell wall polymers of Enterococcus faecalis are critical for resistance to complement activation via mannose-binding lectin","volume":"287","author":"Rooijakkers","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Guerardel, Y., Sadovskaya, I., Maes, E., Furlan, S., Chapot-Chartier, M.P., Mesnage, S., Rigottier-Gois, L., and Serror, P. (2020). Complete structure of the enterococcal polysaccharide antigen (EPA) of vancomycin-resistant enterococcus faecalis v583 reveals that EPA decorations are teichoic acids covalently linked to a rhamnopolysaccharide backbone. MBio, 11.","DOI":"10.1128\/mBio.00277-20"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Teng, F., Jacques-Palaz, K.D., Weinstock, G.M., and Murray, B.E. (2002). Evidence that the Enterococcal Polysaccharide Antigen Gene (epa) Cluster Is Widespread in Enterococcus faecalis and Influences Resistance to Phagocytic Killing of E. faecalis. Infect. Immun., 70.","DOI":"10.1128\/IAI.70.4.2010-2015.2002"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1128\/IAI.68.2.815-823.2000","article-title":"Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis","volume":"68","author":"Xu","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Chatterjee, A., Johnson, C.N., Luong, P., Hullahalli, K., McBride, S.W., Schubert, A.M., Palmer, K.L., Carlson, P.E., and Duerkop, B.A. (2019). Bacteriophage resistance alters antibiotic-mediated intestinal expansion of enterococci. Infect. Immun., 87.","DOI":"10.1128\/IAI.00085-19"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0008-6215(00)83248-6","article-title":"Glycans from streptococcal cell-walls: The molecular structure of an antigenic diheteroglycan of D-glucose and L-rhamnose from Streptococcus bovis","volume":"66","author":"Pazur","year":"1978","journal-title":"Carbohydr. Res."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1002\/chem.201405857","article-title":"Definitive Structural Assessment of Enterococcal Diheteroglycan","volume":"21","author":"Krylov","year":"2015","journal-title":"Chem. Eur. J."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1080\/14760584.2019.1657012","article-title":"Glycoconjugate vaccines: Current approaches towards faster vaccine design","volume":"18","author":"Micoli","year":"2019","journal-title":"Expert Rev. Vaccines"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1039\/D0OB00240B","article-title":"Reagent controlled stereoselective synthesis of teichoic acid \u03b1-(1,2)-glucans","volume":"18","author":"Wang","year":"2020","journal-title":"Org. Biomol. Chem."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"4014","DOI":"10.1002\/chem.201800153","article-title":"Streamlined Synthesis and Evaluation of Teichoic Acid Fragments","volume":"24","author":"Berni","year":"2018","journal-title":"Chem. Eur. J."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Laverde, D., Wobser, D., Romero-Saavedra, F., Hogendorf, W., van der Marel, G., Berthold, M., Kropec, A., Codee, J., and Huebner, J. (2014). Synthetic Teichoic Acid Conjugate Vaccine against Nosocomial Gram-Positive Bacteria. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0110953"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"8961","DOI":"10.1039\/c1cc13132j","article-title":"Automated solid phase synthesis of teichoic acids","volume":"47","author":"Hogendorf","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1021\/acsinfecdis.0c00063","article-title":"Synthetic Oligomers Mimicking Capsular Polysaccharide Diheteroglycan are Potential Vaccine Candidates against Encapsulated Enterococcal Infections","volume":"6","author":"Laverde","year":"2020","journal-title":"ACS Infect. Dis."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"5033","DOI":"10.1128\/IAI.71.9.5033-5041.2003","article-title":"An Enterococcus faecium Secreted Antigen, SagA, Exhibits Broad-Spectrum Binding to Extracellular Matrix Proteins and Appears Essential for E. faecium Growth","volume":"71","author":"Teng","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mib.2017.11.030","article-title":"Evolution of virulence in Enterococcus faecium, a hospital-adapted opportunistic pathogen","volume":"41","author":"Gao","year":"2018","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"6873","DOI":"10.1128\/AEM.01716-15","article-title":"Distinct SagA from Hospital-Associated Clade A1 Enterococcus faecium Strains Contributes to Biofilm Formation","volume":"81","author":"Paganelli","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"3429","DOI":"10.1099\/mic.0.053207-0","article-title":"Identification of SagA as a novel vaccine target for the prevention of Enterococcus faecium infections","volume":"157","author":"Kropec","year":"2011","journal-title":"Microbiology"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Romero-Saavedra, F., Laverde, D., Budin-Verneuil, A., Muller, C., Bernay, B., Benachour, A., Hartke, A., and Huebner, J. (2015). Characterization of two metal binding lipoproteins as vaccine candidates for enterococcal infections. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136625"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"343","DOI":"10.29252\/ibj.23.5.6","article-title":"Molecular cloning and immunogenicity evaluation of PpiC, GeLE, and VS87_01105 proteins of enterococcus faecalis as Vaccine Candidates","volume":"23","author":"Kazemian","year":"2019","journal-title":"Iran. Biomed. J."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"4120","DOI":"10.1128\/IAI.00376-08","article-title":"Contribution of the Collagen Adhesin Acm to Pathogenesis of Enterococcus faecium in Experimental Endocarditis","volume":"76","author":"Nallapareddy","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1128\/IAI.00038-11","article-title":"Relative contributions of Ebp Pili and the collagen adhesin ace to host extracellular matrix protein adherence and experimental urinary tract infection by Enterococcus faecalis OG1RF","volume":"79","author":"Nallapareddy","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"236","DOI":"10.4161\/viru.1.4.11966","article-title":"Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection","volume":"1","author":"Nallapareddy","year":"2010","journal-title":"Virulence"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"3192","DOI":"10.1128\/IAI.02016-06","article-title":"Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm","volume":"75","author":"Nallapareddy","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Sillanp\u00e4\u00e4, J., Chang, C., Singh, K.V., Montealegre, M.C., Nallapareddy, S.R., Harvey, B.R., Ton-That, H., and Murray, B.E. (2013). Contribution of Individual Ebp Pilus Subunits of Enterococcus faecalis OG1RF to Pilus Biogenesis, Biofilm Formation and Urinary Tract Infection. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0068813"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"4484","DOI":"10.1128\/JB.00451-13","article-title":"Pilin and sortase residues critical for endocarditis- and biofilm-associated pilus biogenesis in Enterococcus faecalis","volume":"195","author":"Nielsen","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Flores-Mireles, A.L., Walker, J.N., Potretzke, A., Schreiber, H.L., Pinkner, J.S., Bauman, T.M., Park, A.M., Desai, A., Hultgren, S.J., and Caparon, M.G. (2016). Antibody-based therapy for enterococcal catheter-associated urinary tract infections. MBio, 7.","DOI":"10.1128\/mBio.01653-16"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0167-7799(01)01600-6","article-title":"Antibacterial vaccine design using genomics and proteomics","volume":"19","author":"Grandi","year":"2001","journal-title":"Trends Biotechnol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"3001","DOI":"10.1099\/mic.0.053314-0","article-title":"Lipoproteins of Enterococcus faecalis: Bioinformatic identification, expression analysis and relation to virulence","volume":"157","author":"Reffuveille","year":"2011","journal-title":"Microbiology"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ejps.2018.12.013","article-title":"Semisynthetic glycoconjugate based on dual role protein\/PsaA as a pneumococcal vaccine","volume":"129","author":"Prasanna","year":"2019","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.1574-695X.2002.tb00589.x","article-title":"Identification of ABC transporters in vancomycin-resistant Enterococcus faecium as potential targets for antibody therapy","volume":"33","author":"Burnie","year":"2002","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jprot.2018.05.017","article-title":"Enterococcus faecium produces membrane vesicles containing virulence factors and antimicrobial resistance related proteins","volume":"187","author":"Wagner","year":"2018","journal-title":"J. Proteomics"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Zhang, X., Paganelli, F.L., Bierschenk, D., Kuipers, A., Bonten, M.J.M., Willems, R.J.L., and van Schaik, W. (2012). Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002804"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"12412","DOI":"10.1038\/s41598-018-30882-z","article-title":"Expression profiling in a mammalian host reveals the strong induction of genes encoding LysM domain-containing proteins in Enterococcus faecium","volume":"8","author":"Cacaci","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1128\/IAI.06251-11","article-title":"Involvement of peptidylprolyl cis\/trans isomerases in Enterococcus faecalis virulence","volume":"80","author":"Reffuveille","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1099\/00221287-131-8-1933","article-title":"One or Two Low Affinity Penicillin-binding Proteins May Be Responsible for the Range of Susceptibility of Enterococcus faecium to Benzylpenicillin","volume":"131","author":"Williamson","year":"1985","journal-title":"Microbiology"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1517\/14712598.4.9.1519","article-title":"Treatment and prevention of enterococcal infections-alternative and experimental approaches","volume":"4","author":"Koch","year":"2004","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1086\/338461","article-title":"Patients in Long-Term Care Facilities: A Reservoir for Vancomycin-Resistant Enterococci","volume":"34","author":"Elizaga","year":"2002","journal-title":"Clin. Infect. Dis."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1086\/502016","article-title":"Risk Factors for Vancomycin-Resistant Enterococcus (VRE) Infection in Colonized Patients With Cancer","volume":"23","author":"Husni","year":"2002","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s10719-019-09881-3","article-title":"Detection and characterization of bacterial polysaccharides in drug-resistant enterococci","volume":"36","author":"Ali","year":"2019","journal-title":"Glycoconj. J."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nri2494","article-title":"Maintaining protection against invasive bacteria with protein\u2013polysaccharide conjugate vaccines","volume":"9","author":"Pollard","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/scitranslmed.aat4615","article-title":"Glycoconjugate vaccines: Principles and mechanisms","volume":"10","author":"Rappuoli","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1093\/infdis\/jix315","article-title":"Monoclonal Antibody Protects Against Acinetobacter baumannii Infection by Enhancing Bacterial Clearance and Evading Sepsis","volume":"216","author":"Nielsen","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Diago-Navarro, E., Motley, M.P., Ruiz-Per\u00e9z, G., Yu, W., Austin, J., Seco, B.M.S., Xiao, G., Chikhalya, A., Seeberger, P.H., and Fries, B.C. (2018). Novel, Broadly Reactive Anticapsular Antibodies against Carbapenem-Resistant Klebsiella pneumoniae Protect from Infection. MBio, 9.","DOI":"10.1128\/mBio.00091-18"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1084\/jem.50.4.533","article-title":"Chemo-immunological studies on conjugated carbohydrate-proteins: II. Immunological specificity of synthetic sugar-protein antigens","volume":"50","author":"Avery","year":"1929","journal-title":"J. Exp. Med."},{"key":"ref_121","doi-asserted-by":"crossref","unstructured":"Micoli, F., Adamo, R., and Costantino, P. (2018). Protein Carriers for Glycoconjugate Vaccines: History, Selection Criteria, Characterization and New Trends. Molecules, 23.","DOI":"10.3390\/molecules23061451"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"5513","DOI":"10.1016\/j.vaccine.2010.06.026","article-title":"Glycoconjugate vaccines and immune interference: A review","volume":"28","author":"Dagan","year":"2010","journal-title":"Vaccine"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"4235","DOI":"10.1016\/j.vaccine.2016.06.039","article-title":"Preclinical studies on new proteins as carrier for glycoconjugate vaccines","volume":"34","author":"Tontini","year":"2016","journal-title":"Vaccine"},{"key":"ref_124","doi-asserted-by":"crossref","unstructured":"Romero-Saavedra, F., Laverde, D., Kalfopoulou, E., Martini, C., Torelli, R., Martinez-Matamoros, D., Sanguinetti, M., and Huebner, J. (2019). Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverage. J. Infect. Dis.","DOI":"10.1093\/infdis\/jiz357"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1038\/nrmicro974","article-title":"Passive antibody therapy for infectious diseases","volume":"2","author":"Casadevall","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Kalfopoulou, E., Laverde, D., Miklic, K., Romero-Saavedra, F., Malic, S., Carboni, F., Adamo, R., Lenac Rovis, T., Jonjic, S., and Huebner, J. (2019). Development of Opsonic Mouse Monoclonal Antibodies against Multidrug-Resistant Enterococci. Infect. Immun., 87.","DOI":"10.1128\/IAI.00276-19"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.chembiol.2014.01.002","article-title":"Chemical Biology Approaches to Designing Defined Carbohydrate Vaccines","volume":"21","author":"Anish","year":"2014","journal-title":"Chem. Biol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"235","DOI":"10.2471\/BLT.16.178061","article-title":"Therapeutic antibodies for infectious diseases","volume":"95","author":"Sparrow","year":"2017","journal-title":"Bull. World Health Organ."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Rossmann, F.S., Laverde, D., Kropec, A., Romero-Saavedra, F., Meyer-Buehn, M., and Huebner, J. (2015). Isolation of Highly Active Monoclonal Antibodies against Multiresistant Gram-Positive Bacteria. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0118405"},{"key":"ref_130","doi-asserted-by":"crossref","unstructured":"Singh, K.V., Pinkston, K.L., Gao, P., Harvey, B.R., and Murray, B.E. (2018). Anti-Ace monoclonal antibody reduces Enterococcus faecalis aortic valve infection in a rat infective endocarditis model. Pathog. Dis., 76.","DOI":"10.1093\/femspd\/fty084"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1128\/IAI.01403-13","article-title":"Targeting Pili in Enterococcal Pathogenesis","volume":"82","author":"Pinkston","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"e00397-17","DOI":"10.1128\/mSphere.00397-17","article-title":"A New Take on an Old Remedy: Generating Antibodies against Multidrug-Resistant Gram-Negative Bacteria in a Postantibiotic World","volume":"2","author":"Motley","year":"2017","journal-title":"mSphere"},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.abb.2012.06.001","article-title":"Anti-bacterial monoclonal antibodies: Back to the future?","volume":"526","author":"Oleksiewicz","year":"2012","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"584","DOI":"10.4161\/mabs.3.6.17955","article-title":"Rational identification of an optimal antibody mixture for targeting the epidermal growth factor receptor","volume":"3","author":"Koefoed","year":"2011","journal-title":"MAbs"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s15010-014-0706-1","article-title":"In vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens","volume":"43","author":"Rossmann","year":"2015","journal-title":"Infection"}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/11\/2397\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:28:04Z","timestamp":1760178484000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/11\/2397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,2]]},"references-count":135,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["cells9112397"],"URL":"https:\/\/doi.org\/10.3390\/cells9112397","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,2]]}}}