{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:45:07Z","timestamp":1789202707160,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Telethon Foundation (to E.D.S.)","award":["GGP16203"],"award-info":[{"award-number":["GGP16203"]}]},{"name":"The SMA Trust (now SMA UK) (to M.B.)","award":["-"],"award-info":[{"award-number":["-"]}]},{"name":"Deutsche Muskelstiftung (DMS)\/ Philipp &amp; Freunde - SMA Deutschland (to P.C.)","award":["-"],"award-info":[{"award-number":["-"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Spinal Muscular Atrophy (SMA) is a neuromuscular disease caused by decreased levels of the survival of motoneuron (SMN) protein. Post-translational mechanisms for regulation of its stability are still elusive. Thus, we aimed to identify regulatory phosphorylation sites that modulate function and stability. Our results show that SMN residues S290 and S292 are phosphorylated, of which SMN pS290 has a detrimental effect on protein stability and nuclear localization. Furthermore, we propose that phosphatase and tensin homolog (PTEN), a novel phosphatase for SMN, counteracts this effect. In light of recent advancements in SMA therapies, a significant need for additional approaches has become apparent. Our study demonstrates S290 as a novel molecular target site to increase the stability of SMN. Characterization of relevant kinases and phosphatases provides not only a new understanding of SMN function, but also constitutes a novel strategy for combinatorial therapeutic approaches to increase the level of SMN in SMA.<\/jats:p>","DOI":"10.3390\/cells9112405","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T09:09:32Z","timestamp":1604394572000},"page":"2405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Single Amino Acid Residue Regulates PTEN-Binding and Stability of the Spinal Muscular Atrophy Protein SMN"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1592-9838","authenticated-orcid":false,"given":"Sebastian","family":"Rademacher","sequence":"first","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School, 30625 Hannover, 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4094-9996","authenticated-orcid":false,"given":"Pamela","family":"Santonicola","sequence":"additional","affiliation":[{"name":"Institute of Biosciences and Bioresources, National Research Council of Italy, 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga-Maria","family":"Wefel","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janina","family":"Dehus","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical School, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa M.","family":"Walter","sequence":"additional","affiliation":[{"name":"Institute of Neuroanatomy and Cell Biology, Hannover Medical 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Institute of Biochemistry, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Varela","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK"},{"name":"Department of Paediatrics, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-6403","authenticated-orcid":false,"given":"Melissa","family":"Bowerman","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3QX, UK"},{"name":"School of Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Wolfson Centre for Inherited Neuromuscular 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