{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:45:28Z","timestamp":1792640728290,"version":"4.5.0"},"reference-count":61,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T00:00:00Z","timestamp":1606953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Federal Ministry of Education and Research (BMBF)","award":["No.03Z1L511 (SONO-RAY project)"],"award-info":[{"award-number":["No.03Z1L511 (SONO-RAY project)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Focused ultrasound (FUS) has become an important non-invasive therapy for solid tumor ablation via thermal effects. The cavitation effect induced by FUS is thereby avoided but applied for lithotripsy, support drug delivery and the induction of blood vessel destruction for cancer therapy. In this study, head and neck cancer (FaDu), glioblastoma (T98G), and prostate cancer (PC-3) cells were exposed to FUS by using an in vitro FUS system followed by single-dose X-ray radiation therapy (RT) or water bath hyperthermia (HT). Sensitization effects of short FUS shots with cavitation (FUS-Cav) or without cavitation (FUS) to RT or HT (45 \u00b0C, 30 min) were evaluated. FUS-Cav significantly increases the sensitivity of cancer cells to RT and HT by reducing long-term clonogenic survival, short-term cell metabolic activity, cell invasion, and induction of sonoporation. Our results demonstrated that short FUS treatment with cavitation has good potential to sensitize cancer cells to RT and HT non-invasively.<\/jats:p>","DOI":"10.3390\/cells9122595","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T20:09:40Z","timestamp":1607026180000},"page":"2595","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Focused Ultrasound-Induced Cavitation Sensitizes Cancer Cells to Radiation Therapy and Hyperthermia"],"prefix":"10.3390","volume":"9","author":[{"given":"Shaonan","family":"Hu","sequence":"first","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Unger","sequence":"additional","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Patties","sequence":"additional","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"},{"name":"Department of Radiation Oncology, University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Melzer","sequence":"additional","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"},{"name":"Institute for Medical Science and Technology (IMSaT), University of Dundee, Dundee DD2 1FD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Landgraf","sequence":"additional","affiliation":[{"name":"Innovation Center Computer Assisted Surgery (ICCAS), University of Leipzig, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1055\/a-0817-5645","article-title":"Magnetic Resonance-Guided High-Intensity Focused Ultrasound (MR-HIFU): Technical Background and Overview of Current Clinical Applications (Part 1)","volume":"191","author":"Siedek","year":"2019","journal-title":"R\u00f6Fo\u2014Fortschr. Geb. R\u00f6ntgenstrahlen Bildgeb. Verfahr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1080\/02656736.2018.1433879","article-title":"Real-time 3D ultrasound based motion tracking for the treatment of mobile organs with MR-guided high-intensity focused ultrasound","volume":"34","author":"Bour","year":"2018","journal-title":"Int. J. Hyperth."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Elming, P.B., S\u00f8rensen, B.S., Oei, A.L., Franken, N.A., Crezee, J., Overgaard, J., and Horsman, M.R. (2019). Hyperthermia: The Optimal Treatment to Overcome Radiation Resistant Hypoxia. Cancers, 11.","DOI":"10.3390\/cancers11010060"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1080\/0265673032000173898","article-title":"Introduction: Radiosensitization by hyperthermia","volume":"20","author":"Roti","year":"2004","journal-title":"Int. J. Hyperth."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3389\/fonc.2017.00132","article-title":"Integrating Hyperthermia into Modern Radiation Oncology: What Evidence Is Necessary?","volume":"7","author":"Peeken","year":"2017","journal-title":"Front. Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Baronzio, G., Parmar, G., Ballerini, M., Szasz, A., and Cassutti, V. (2014). A Brief Overview of Hyperthermia in Cancer Treatment. J. Integr. Oncol., 3.","DOI":"10.4172\/2329-6771.1000115"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"136","DOI":"10.18433\/J3ZP5F","article-title":"High intensity focused ultrasound technology, its scope and applications in therapy and drug delivery","volume":"17","author":"Phenix","year":"2014","journal-title":"J. Pharm. Pharm. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2406","DOI":"10.1016\/j.ultrasmedbio.2013.06.010","article-title":"A Comparison of Acoustic Cavitation Detection Thresholds Measured with Piezo-electric and Fiber-optic Hydrophone Sensors","volume":"39","author":"Bull","year":"2013","journal-title":"Ultrasound Med. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nphys148","article-title":"Membrane disruption by optically controlled microbubble cavitation","volume":"1","author":"Prentice","year":"2005","journal-title":"Nat. Phys."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ultras.2009.10.002","article-title":"Cavitation microstreaming and stress fields created by microbubbles","volume":"50","author":"Collis","year":"2010","journal-title":"Ultrasonics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.fluid.40.111406.102116","article-title":"Applications of Acoustics and Cavitation to Noninvasive Therapy and Drug Delivery","volume":"40","author":"Coussios","year":"2008","journal-title":"Annu. Rev. Fluid Mech."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/j.ultrasmedbio.2015.03.016","article-title":"In Vitro Investigation of the Individual Contributions of Ultrasound-Induced Stable and Inertial Cavitation in Targeted Drug Delivery","volume":"41","author":"Gourevich","year":"2015","journal-title":"Ultrasound Med. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ddtec.2016.07.007","article-title":"Ultrasound-mediated drug delivery to the brain: Principles, progress and prospects","volume":"20","author":"Dasgupta","year":"2016","journal-title":"Drug Discov. Today Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1121\/1.4788649","article-title":"Disintegration of Tissue Using High Intensity Focused Ultrasound: Two Approaches That Utilize Shock Waves","volume":"8","author":"Maxwell","year":"2012","journal-title":"Acoust. Today"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40349-017-0089-8","article-title":"Multi-focal HIFU reduces cavitation in mild-hyperthermia","volume":"5","author":"Chaplin","year":"2017","journal-title":"J. Ther. Ultrasound"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"034302","DOI":"10.1063\/1.4978811","article-title":"A multimodal instrument for real-time in situ study of ultrasound and cavitation mediated drug delivery","volume":"88","author":"Bian","year":"2017","journal-title":"Rev. Sci. Instrum."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"044902","DOI":"10.1063\/1.3579499","article-title":"Laser-nucleated acoustic cavitation in focused ultrasound","volume":"82","author":"Gerold","year":"2011","journal-title":"Rev. Sci. Instrum."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.ultrasmedbio.2009.09.010","article-title":"Shock-Induced Heating and Millisecond Boiling in Gels and Tissue Due to High Intensity Focused Ultrasound","volume":"36","author":"Canney","year":"2010","journal-title":"Ultrasound Med. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1148\/radiol.11110670","article-title":"Spatiotemporal Monitoring of High-Intensity Focused Ultrasound Therapy with Passive Acoustic Mapping","volume":"262","author":"Jensen","year":"2012","journal-title":"Radiology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"194301","DOI":"10.1103\/PhysRevLett.102.194301","article-title":"Focusing Ultrasound with an Acoustic Metamaterial Network","volume":"102","author":"Zhang","year":"2009","journal-title":"Phys. Rev. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.ultsonch.2008.09.007","article-title":"Assay of hydroxyl radicals generated by focused ultrasound","volume":"16","author":"Villeneuve","year":"2009","journal-title":"Ultrason. Sonochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"98","DOI":"10.18632\/oncoscience.299","article-title":"Breast tumor response to ultrasound mediated excitation of microbubbles and radiation therapy in vivo","volume":"3","author":"Lai","year":"2016","journal-title":"Oncoscience"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhang, X., Bobeica, M., Unger, M., Bednarz, A., Gerold, B., Patties, I., Melzer, A., and Landgraf, L. (2020). Focused ultrasound radiosensitize human cancer cells by enhancement of DNA damage, Submitted.","DOI":"10.1007\/s00066-021-01774-5"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.ultsonch.2006.12.016","article-title":"Effect of exposure parameters on cavitation induced by low-level dual-frequency ultrasound","volume":"14","author":"Barati","year":"2007","journal-title":"Ultrason. Sonochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1200\/JCO.18.00622","article-title":"Safety and Efficacy of a Five-Fraction Stereotactic Body Radiotherapy Schedule for Centrally Located Non\u2013Small-Cell Lung Cancer: NRG Oncology\/RTOG 0813 Trial","volume":"37","author":"Bezjak","year":"2019","journal-title":"J. Clin. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1097\/01243894-200610000-00008","article-title":"Results of a Phase I Dose-Escalation Study Using Single-Fraction Stereotactic Radiotherapy for Lung Tumors","volume":"1","author":"Le","year":"2006","journal-title":"J. Thorac. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1038\/nprot.2006.339","article-title":"Clonogenic assay of cells in vitro","volume":"1","author":"Franken","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1186\/s13046-019-1434-2","article-title":"The Chk1 inhibitor SAR-020106 sensitizes human glioblastoma cells to irradiation, to temozolomide, and to decitabine treatment","volume":"38","author":"Patties","year":"2019","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1385\/1-59259-860-9:097","article-title":"Tumor Cell Invasion Assays","volume":"294","author":"Shaw","year":"2004","journal-title":"Cell Migration"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.3892\/ol.2013.1389","article-title":"Sonoporation by low-frequency and low-power ultrasound enhances chemotherapeutic efficacy in prostate cancer cells in vitro","volume":"6","author":"Wang","year":"2013","journal-title":"Oncol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.ultrasmedbio.2018.12.007","article-title":"Ultrasound Hyperthermia Technology for Radiosensitization","volume":"45","author":"Zhu","year":"2019","journal-title":"Ultrasound Med. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1146\/annurev.bioeng.6.040803.140126","article-title":"Mechanical Bioeffects of Ultrasound","volume":"6","author":"Dalecki","year":"2004","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ali, E.A., Bordacahar, B., Mestas, J.-L., Batteux, F., Lafon, C., Camus, M., and Prat, F. (2018). Ultrasonic cavitation induces necrosis and impairs growth in three-dimensional models of pancreatic ductal adenocarcinoma. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0209094"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e41","DOI":"10.1097\/GOX.0b013e3182a7f222","article-title":"Histological and Ultrastructural Effects of Ultrasound-induced Cavitation on Human Skin Adipose Tissue","volume":"1","author":"Bani","year":"2013","journal-title":"Plast. Reconstr. Surg. Glob. Open"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1517\/17425247.3.6.713","article-title":"Ultrasound-induced cavitation: Applications in drug and gene delivery","volume":"3","author":"Paliwal","year":"2006","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.jacbts.2017.07.011","article-title":"Ultrasound-Induced Microbubble Cavitation for the Treatment of Catheterization-Induced Vasospasm","volume":"2","author":"Kutty","year":"2017","journal-title":"JACC Basic Transl. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3109\/02656736.2015.1014434","article-title":"Heat and sound: Focused ultrasound in the clinic","volume":"31","year":"2015","journal-title":"Int. J. Hyperth."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ultrasmedbio.2017.09.005","article-title":"Interstitial Matrix Prevents Therapeutic Ultrasound From Causing Inertial Cavitation in Tumescent Subcutaneous Tissue","volume":"44","author":"Koulakis","year":"2018","journal-title":"Ultrasound Med. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1080\/02656730701194131","article-title":"Role of acoustic cavitation in the delivery and monitoring of cancer treatment by high-intensity focused ultrasound (HIFU)","volume":"23","author":"Coussios","year":"2007","journal-title":"Int. J. Hyperth."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1007\/s11517-009-0471-y","article-title":"High intensity focused ultrasound lithotripsy with cavitating microbubbles","volume":"47","author":"Yoshizawa","year":"2009","journal-title":"Med. Biol. Eng. Comput."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1080\/17425247.2018.1547279","article-title":"Sonopermeation to improve drug delivery to tumors: From fundamental understanding to clinical translation","volume":"15","author":"Snipstad","year":"2018","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-018-04529-6","article-title":"Blood\u2013brain barrier opening in Alzheimer\u2019s disease using MR-guided focused ultrasound","volume":"9","author":"Lipsman","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1016\/j.ultrasmedbio.2006.07.016","article-title":"Correlation between inertial cavitation dose and endothelial cell damage in vivo","volume":"32","author":"Hwang","year":"2006","journal-title":"Ultrasound Med. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0301-5629(01)00356-8","article-title":"A comparison of the hemolytic potential of Optison and Albunex in whole human blood in vitro: Acoustic pressure, ultrasound frequency, donor and passive cavitation detection considerations","volume":"27","author":"Miller","year":"2001","journal-title":"Ultrasound Med. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1109\/TUFFC.2014.2992","article-title":"Transcranial cavitation detection in primates during blood-brain barrier opening-a performance assessment study","volume":"61","author":"Wu","year":"2014","journal-title":"IEEE Trans. Ultrason. Ferroelectr. Freq. Control."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1016\/j.ultrasmedbio.2019.06.410","article-title":"Focused Ultrasound-Mediated Hyperthermia in Vitro: An Experimental Arrangement for Treating Cells under Tissue-Mimicking Conditions","volume":"45","author":"Rivens","year":"2019","journal-title":"Ultrasound Med. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1016\/j.ultrasmedbio.2018.01.021","article-title":"Influence of Acoustic Reflection on the Inertial Cavitation Dose in a Franz Diffusion Cell","volume":"44","author":"Robertson","year":"2018","journal-title":"Ultrasound Med. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"44593","DOI":"10.18632\/oncotarget.17861","article-title":"The effect of thermal dose on hyperthermia-mediated inhibition of DNA repair through homologous recombination","volume":"8","author":"Tempel","year":"2017","journal-title":"Oncotarget"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1016\/j.ultrasmedbio.2013.03.025","article-title":"Ultrasound-Mediated Microbubble Enhancement of Radiation Therapy Studied Using Three-Dimensional High-Frequency Power Doppler Ultrasound","volume":"39","author":"Kwok","year":"2013","journal-title":"Ultrasound Med. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.ultrasmedbio.2017.01.023","article-title":"Mechanical and Biological Effects of Ultrasound: A Review of Present Knowledge","volume":"43","author":"Izadifar","year":"2017","journal-title":"Ultrasound Med. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.canlet.2017.09.037","article-title":"Localized microbubble cavitation-based antivascular therapy for improving HCC treatment response to radiotherapy","volume":"411","author":"Daecher","year":"2017","journal-title":"Cancer Lett."},{"key":"ref_52","first-page":"763","article-title":"Radioresistance in Head and Neck Squamous Cell Carcinoma \u2014 Possible Molecular Markers for Local Recurrence and New Putative Therapeutic Strategies","volume":"37","author":"Ganci","year":"2015","journal-title":"Contemp. Issues Head Neck Cancer Manag."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"531","DOI":"10.3109\/02656736.2014.968640","article-title":"Local tumour hyperthermia as immunotherapy for metastatic cancer","volume":"30","author":"Fiering","year":"2014","journal-title":"Int. J. Hyperth."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1080\/02656736.2018.1514468","article-title":"Microbubble-assisted MRI-guided focused ultrasound for hyperthermia at reduced power levels","volume":"35","author":"Santos","year":"2018","journal-title":"Int. J. Hyperth."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"53","DOI":"10.7785\/tcrt.2012.500253","article-title":"Ultrasound-Activated Microbubble Cancer Therapy: Ceramide Production Leading to Enhanced Radiation Effect in vitro","volume":"12","author":"Nofiele","year":"2013","journal-title":"Technol. Cancer Res. Treat."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"C181","DOI":"10.1152\/ajpcell.00087.2015","article-title":"The plasma membrane as a capacitor for energy and metabolism","volume":"310","author":"Ray","year":"2016","journal-title":"Am. J. Physiol. Physiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jconrel.2006.02.007","article-title":"Vibrating microbubbles poking individual cells: Drug transfer into cells via sonoporation","volume":"112","author":"Kooiman","year":"2006","journal-title":"J. Control. Release"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1016\/j.ultrasmedbio.2009.05.012","article-title":"The Size of Sonoporation Pores on the Cell Membrane","volume":"35","author":"Zhou","year":"2009","journal-title":"Ultrasound Med. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.ultrasmedbio.2008.06.007","article-title":"Induction of Apoptosis in Sonoporation and Ultrasonic Gene Transfer","volume":"35","author":"Miller","year":"2009","journal-title":"Ultrasound Med. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1016\/j.ultrasmedbio.2011.09.012","article-title":"Sonoporation Induces Apoptosis and Cell Cycle Arrest in Human Promyelocytic Leukemia Cells","volume":"37","author":"Zhong","year":"2011","journal-title":"Ultrasound Med. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jconrel.2008.07.038","article-title":"Experimental study on cell self-sealing during sonoporation","volume":"131","author":"Yang","year":"2008","journal-title":"J. Control. 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