{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:44:15Z","timestamp":1790721855451,"version":"4.1.0"},"reference-count":76,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP17bm0804014, JP18bm0804014, JP19bm0804014, JP15bm0609003, JP16bm0609003, JP17bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 , and JP20bm0804003"],"award-info":[{"award-number":["JP17bm0804014, JP18bm0804014, JP19bm0804014, JP15bm0609003, JP16bm0609003, JP17bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 , and JP20bm0804003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP17bm0804014, JP18bm0804014, JP19bm0804014, JP15bm0609003, JP16bm0609003, JP17bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 , JP20bm0804003, JP17ek0109139, JP15bk0104027, JP 16bk0104016, JP 17bk0104016"],"award-info":[{"award-number":["JP17bm0804014, JP18bm0804014, JP19bm0804014, JP15bm0609003, JP16bm0609003, JP17bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 , JP20bm0804003, JP17ek0109139, JP15bk0104027, JP 16bk0104016, JP 17bk0104016"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Japan Society for the Promotion of Science KAKENHI","award":["15J03921, 19K17002, 17K09762, 19K08002, 20K16502"],"award-info":[{"award-number":["15J03921, 19K17002, 17K09762, 19K08002, 20K16502"]}]},{"name":"Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Induced pluripotent stem cell (iPSC)-based disease modeling has a great potential for uncovering the mechanisms of pathogenesis, especially in the case of neurodegenerative diseases where disease-susceptible cells can usually not be obtained from patients. So far, the iPSC-based modeling of neurodegenerative diseases has mainly focused on neurons because the protocols for generating astrocytes from iPSCs have not been fully established. The growing evidence of astrocytes\u2019 contribution to neurodegenerative diseases has underscored the lack of iPSC-derived astrocyte models. In the present study, we established a protocol to efficiently generate iPSC-derived astrocytes (iPasts), which were further characterized by RNA and protein expression profiles as well as functional assays. iPasts exhibited calcium dynamics and glutamate uptake activity comparable to human primary astrocytes. Moreover, when co-cultured with neurons, iPasts enhanced neuronal synaptic maturation. Our protocol can be used for modeling astrocyte-related disease phenotypes in vitro and further exploring the contribution of astrocytes to neurodegenerative diseases.<\/jats:p>","DOI":"10.3390\/cells9122680","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:45:36Z","timestamp":1607906736000},"page":"2680","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Human Astrocytes Model Derived from Induced Pluripotent Stem Cells"],"prefix":"10.3390","volume":"9","author":[{"given":"Nicolas","family":"Leventoux","sequence":"first","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent","family":"Imaizumi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Sato","sequence":"additional","affiliation":[{"name":"Keio University Graduate School of Science and Technology, Kanagawa 223-8522, Japan"},{"name":"Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Wako City, Saitama 351-0198, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0903-3891","authenticated-orcid":false,"given":"Shinichi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"},{"name":"Department of Neurology and Stroke, Saitama Medical University International Medical Center, 1397-1 Yamane, Hidaka-shi, Saitama 350-1298, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Mashima","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iki","family":"Sonn","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4641-7640","authenticated-orcid":false,"given":"Takahiro","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaka","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7482-5935","authenticated-orcid":false,"given":"Hideyuki","family":"Okano","sequence":"additional","affiliation":[{"name":"Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4115","DOI":"10.1523\/JNEUROSCI.5308-07.2008","article-title":"Mitochondrial Dysfunction in SOD1G93A-Bearing Astrocytes Promotes Motor Neuron Degeneration: Prevention by Mitochondrial-Targeted Antioxidants","volume":"28","author":"Cassina","year":"2008","journal-title":"J. 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