{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T12:03:34Z","timestamp":1792325014487,"version":"4.4.0"},"reference-count":97,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T00:00:00Z","timestamp":1608249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["NA400\/5; NA400\/7; NA400\/9; NA400\/10"],"award-info":[{"award-number":["NA400\/5; NA400\/7; NA400\/9; NA400\/10"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","award":["M.KF.A.BIOC0001"],"award-info":[{"award-number":["M.KF.A.BIOC0001"]}],"id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Fibronectin is a ubiquitous extracellular matrix protein that is produced by many cell types in the bone marrow and distributed throughout it. Cells of the stem cell niche produce the various isoforms of this protein. Fibronectin not only provides the cells a scaffold to bind to, but it also modulates their behavior by binding to receptors on the adjacent hematopoietic stem cells and stromal cells. These receptors, which include integrins such as \u03b14\u03b21, \u03b19\u03b21, \u03b14\u03b27, \u03b15\u03b21, \u03b1v\u03b23, Toll-like receptor-4 (TLR-4), and CD44, are found on the hematopoietic stem cell. Because the knockout of fibronectin is lethal during embryonal development and because fibronectin is produced by almost all cell types in mammals, the study of its role in hematopoiesis is difficult. Nevertheless, strong and direct evidence exists for its stimulation of myelopoiesis and thrombopoiesis using in vivo models. Other reviewed effects can be deduced from the study of fibronectin receptors, which showed their activation modifies the behavior of hematopoietic stem cells. Erythropoiesis was only stimulated under hemolytic stress, and mostly late stages of lymphocytic differentiation were modulated. Because fibronectin is ubiquitously expressed, these interactions in health and disease need to be taken into account whenever any molecule is evaluated in hematopoiesis.<\/jats:p>","DOI":"10.3390\/cells9122717","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T04:12:01Z","timestamp":1608523921000},"page":"2717","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Fibronectin and Its Receptors in Hematopoiesis"],"prefix":"10.3390","volume":"9","author":[{"given":"Franziska","family":"Wirth","sequence":"first","affiliation":[{"name":"Institute of Immunology, University of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Lubosch","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hamelmann","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inaam A.","family":"Nakchbandi","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University of Heidelberg, 69120 Heidelberg, Germany"},{"name":"Max-Planck Institute for Medical Research, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/nature02040","article-title":"Osteoblastic cells regulate the haematopoietic stem cell niche","volume":"425","author":"Calvi","year":"2003","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1089\/ars.2017.7419","article-title":"Where Hematopoietic Stem Cells Live: The Bone Marrow Niche","volume":"29","author":"Szade","year":"2018","journal-title":"Antioxid. Redox Signal."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nature11885","article-title":"Haematopoietic stem cells and early lymphoid progenitors occupy distinct bone marrow niches","volume":"495","author":"Ding","year":"2013","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"18871","DOI":"10.1074\/jbc.M410762200","article-title":"Fibronectin regulates latent transforming growth factor-beta (TGF beta) by controlling matrix assembly of latent TGF beta-binding protein-1","volume":"280","author":"Dallas","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1002\/jcp.24877","article-title":"Absence of bone sialoprotein (BSP) alters profoundly hematopoiesis and upregulates osteopontin","volume":"230","author":"Granito","year":"2015","journal-title":"J. Cell. Physiol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cao, H., Cao, B., Heazlewood, C.K., Domingues, M., Sun, X., Debele, E., McGregor, N.E., Sims, N.A., Heazlewood, S.Y., and Nilsson, S.K. (2019). Osteopontin is an important regulative component of the fetal bone marrow hematopoietic stem cell niche. Cells, 8.","DOI":"10.3390\/cells8090985"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1182\/blood-2010-08-301119","article-title":"In vitro requirement for periostin in B lymphopoiesis","volume":"117","author":"Siewe","year":"2011","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1007\/s10456-017-9563-8","article-title":"Endothelium-derived fibronectin regulates neonatal vascular morphogenesis in an autocrine fashion","volume":"20","author":"Turner","year":"2017","journal-title":"Angiogenesis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1593\/neo.13762","article-title":"Circulating fibronectin controls tumor growth","volume":"15","author":"Vasel","year":"2013","journal-title":"Neoplasia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s00441-016-2397-7","article-title":"Blood-brain barrier properties in vitro depend on composition and assembly of endogenous extracellular matrices","volume":"365","author":"Zobel","year":"2016","journal-title":"Cell Tissue Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1359\/jbmr.091011","article-title":"Circulating fibronectin affects bone matrix, whereas osteoblast fibronectin modulates osteoblast function","volume":"25","author":"Bentmann","year":"2010","journal-title":"J. Bone Min. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7745","DOI":"10.1074\/jbc.M116.739987","article-title":"Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins","volume":"292","author":"Sens","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1158\/0008-5472.CAN-16-3507","article-title":"A subpopulation of stromal cells controls cancer cell homing to the bone marrow","volume":"78","author":"Rossnagl","year":"2018","journal-title":"Cancer Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1359\/jbmr.080313","article-title":"Isoform of fibronectin mediates bone loss in patients with primary biliary cirrhosis by suppressing bone formation","volume":"23","author":"Kawelke","year":"2008","journal-title":"J. Bone Min. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kawelke, N., Vasel, M., Sens, C., von Au, A., Dooley, S., and Nakchbandi, I.A. (2011). Fibronectin protects from excessive liver fibrosis by modulating the availability of and responsiveness of stellate cells to active TGF-beta. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028181"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.ceb.2008.06.001","article-title":"The role of integrin binding sites in fibronectin matrix assembly in vivo","volume":"20","author":"Leiss","year":"2008","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/jbmr.2916","article-title":"An O-glycosylation of fibronectin mediates hepatic osteodystrophy through alpha4beta1 integrin","volume":"32","author":"Sens","year":"2017","journal-title":"J. Bone Min. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1242\/dev.119.4.1079","article-title":"Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin","volume":"119","author":"George","year":"1993","journal-title":"Development"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rossnagl, S., Altrock, E., Sens, C., Kraft, S., Rau, K., Milsom, M.D., Giese, T., Samstag, Y., and Nakchbandi, I.A. (2016). EDA-fibronectin originating from osteoblasts inhibits the immune response against cancer. PLoS Biol., 14.","DOI":"10.1371\/journal.pbio.1002562"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1242\/jcs.018093","article-title":"Integrin signalling at a glance","volume":"122","author":"Harburger","year":"2009","journal-title":"J. Cell Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/sj.bmt.1705534","article-title":"Differential role for very late antigen-5 in mobilization and homing of hematopoietic stem cells","volume":"38","author":"Wierenga","year":"2006","journal-title":"Bone Marrow Transpl."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1182\/blood-2009-01-197988","article-title":"Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins","volume":"114","author":"Grassinger","year":"2009","journal-title":"Blood"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1182\/blood-2005-10-007658","article-title":"Adult murine hematopoiesis can proceed without beta1 and beta7 integrins","volume":"108","author":"Bungartz","year":"2006","journal-title":"Blood"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.3324\/haematol.2009.006072","article-title":"The integrin alpha9beta1 on hematopoietic stem and progenitor cells: Involvement in cell adhesion, proliferation and differentiation","volume":"94","author":"Schreiber","year":"2009","journal-title":"Haematologica"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1182\/blood-2011-02-335430","article-title":"Integrin-alphavbeta3 regulates thrombopoietin-mediated maintenance of hematopoietic stem cells","volume":"119","author":"Umemoto","year":"2012","journal-title":"Blood"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.immuni.2006.04.008","article-title":"Toll-like receptors on hematopoietic progenitor cells stimulate innate immune system replenishment","volume":"24","author":"Nagai","year":"2006","journal-title":"Immunity"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"5367","DOI":"10.4049\/jimmunol.1003438","article-title":"Chronic exposure to a TLR ligand injures hematopoietic stem cells","volume":"186","author":"Esplin","year":"2011","journal-title":"J. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3324\/haematol.2015.135921","article-title":"The role of CD44 in fetal and adult hematopoietic stem cell regulation","volume":"101","author":"Cao","year":"2016","journal-title":"Haematologica"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1083\/jcb.139.1.295","article-title":"Modulation of cell-adhesive activity of fibronectin by the alternatively spliced EDA segment","volume":"139","author":"Manabe","year":"1997","journal-title":"J. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"14467","DOI":"10.1074\/jbc.M201100200","article-title":"The EIIIA segment of fibronectin is a ligand for integrins alpha 9beta 1 and alpha 4beta 1 providing a novel mechanism for regulating cell adhesion by alternative splicing","volume":"277","author":"Liao","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1074\/jbc.M708306200","article-title":"Identification of the peptide sequences within the EIIIA (EDA) segment of fibronectin that mediate integrin alpha9beta1-dependent cellular activities","volume":"283","author":"Shinde","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1007\/s00109-015-1373-0","article-title":"Identification and characterization of a unique role for EDB fibronectin in phagocytosis","volume":"94","author":"Kraft","year":"2016","journal-title":"J. Mol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1083\/jcb.200410068","article-title":"Three-dimensional EM structure of the ectodomain of integrin {alpha}V{beta}3 in a complex with fibronectin","volume":"168","author":"Adair","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"36466","DOI":"10.1074\/jbc.M604812200","article-title":"Spontaneous formation of L-isoaspartate and gain of function in fibronectin","volume":"281","author":"Curnis","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"10229","DOI":"10.1074\/jbc.M100099200","article-title":"The extra domain A of fibronectin activates Toll-like receptor 4","volume":"276","author":"Okamura","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"36255","DOI":"10.1074\/jbc.C110.176990","article-title":"The first type III repeat in fibronectin activates an inflammatory pathway in dermal fibroblasts","volume":"285","author":"You","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"349","DOI":"10.3109\/00365520903490606","article-title":"Circulating fibronectin isoforms predict the degree of fibrosis in chronic hepatitis C","volume":"45","author":"Hackl","year":"2010","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1097\/MOH.0b013e3283476140","article-title":"The biology of CD44 and HCELL in hematopoiesis: The \u2019step 2-bypass pathway\u2019 and other emerging perspectives","volume":"18","author":"Sackstein","year":"2011","journal-title":"Curr. Opin. Hematol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1802","DOI":"10.1182\/blood.V84.6.1802.1802","article-title":"Adhesion of committed human hematopoietic progenitors to synthetic peptides from the C-terminal heparin-binding domain of fibronectin: Cooperation between the integrin alpha 4 beta 1 and the CD44 adhesion receptor","volume":"84","author":"Verfaillie","year":"1994","journal-title":"Blood"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9410","DOI":"10.1074\/jbc.275.13.9410","article-title":"Heparan sulfate chains from glypican and syndecans bind the Hep II domain of fibronectin similarly despite minor structural differences","volume":"275","author":"Tumova","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.devcel.2013.01.027","article-title":"Syndecan-4 phosphorylation is a control point for integrin recycling","volume":"24","author":"Morgan","year":"2013","journal-title":"Dev. Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"47483","DOI":"10.1074\/jbc.M106268200","article-title":"Syndecan-4 deficiency leads to high mortality of lipopolysaccharide-injected mice","volume":"276","author":"Ishiguro","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1242\/jcs.115.1.227","article-title":"Syndecan-4 mediates antithrombin-induced chemotaxis of human peripheral blood lymphocytes and monocytes","volume":"115","author":"Kaneider","year":"2002","journal-title":"J. Cell Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0092-8674(94)90329-8","article-title":"Developmental switches in the immune system","volume":"76","author":"Weissman","year":"1994","journal-title":"Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"338","DOI":"10.2353\/ajpath.2006.060312","article-title":"Hematopoietic stem cells: The paradigmatic tissue-specific stem cell","volume":"169","author":"Bryder","year":"2006","journal-title":"Am. J. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1084\/jem.20150269","article-title":"Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells","volume":"212","author":"Ruppert","year":"2015","journal-title":"J. Exp. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S1074-7613(00)80046-1","article-title":"The chemokine receptor CXCR4 is required for the retention of B lineage and granulocytic precursors within the bone marrow microenvironment","volume":"10","author":"Ma","year":"1999","journal-title":"Immunity"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/BF02982544","article-title":"Regulation of hematopoiesis by chemokine family members","volume":"74","author":"Broxmeyer","year":"2001","journal-title":"Int. J. Hematol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1182\/blood.V93.1.149","article-title":"Selective transendothelial migration of hematopoietic progenitor cells: A role in homing of progenitor cells","volume":"93","author":"Imai","year":"1999","journal-title":"Blood"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1016\/j.bbrc.2006.09.140","article-title":"Fibronectin and laminin enhance engraftibility of cultured hematopoietic stem cells","volume":"350","author":"Sagar","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/S1074-7613(00)80216-2","article-title":"Fetal and adult hematopoietic stem cells require beta1 integrin function for colonizing fetal liver, spleen, and bone marrow","volume":"12","author":"Potocnik","year":"2000","journal-title":"Immunity"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S1074-7613(00)80131-4","article-title":"Alpha4 integrins regulate the proliferation\/differentiation balance of multilineage hematopoietic progenitors in vivo","volume":"11","author":"Arroyo","year":"1999","journal-title":"Immunity"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"9647","DOI":"10.1073\/pnas.92.21.9647","article-title":"The VLA4\/VCAM-1 adhesion pathway defines contrasting mechanisms of lodgement of transplanted murine hemopoietic progenitors between bone marrow and spleen","volume":"92","author":"Papayannopoulou","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1182\/blood-2003-12-4157","article-title":"Integrin alpha4beta7 and its counterreceptor MAdCAM-1 contribute to hematopoietic progenitor recruitment into bone marrow following transplantation","volume":"104","author":"Katayama","year":"2004","journal-title":"Blood"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"9349","DOI":"10.1128\/MCB.23.24.9349-9360.2003","article-title":"Deletion of alpha4 integrins from adult hematopoietic cells reveals roles in homeostasis, regeneration, and homing","volume":"23","author":"Scott","year":"2003","journal-title":"Mol. Cell Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nm1703","article-title":"Ex vivo glycan engineering of CD44 programs human multipotent mesenchymal stromal cell trafficking to bone","volume":"14","author":"Sackstein","year":"2008","journal-title":"Nat. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/978-1-61779-145-1_13","article-title":"Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors","volume":"750","author":"Cancelas","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"9374","DOI":"10.1073\/pnas.90.20.9374","article-title":"Peripheralization of hemopoietic progenitors in primates treated with anti-VLA4 integrin","volume":"90","author":"Papayannopoulou","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"13500","DOI":"10.1038\/ncomms13500","article-title":"Outside-in integrin signalling regulates haematopoietic stem cell function via Periostin-Itgav axis","volume":"7","author":"Khurana","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.4049\/jimmunol.1501231","article-title":"Cutting edge: Hematopoietic stem cell expansion and common lymphoid progenitor depletion require hematopoietic-derived, cell-autonomous TLR4 in a model of chronic endotoxin","volume":"195","author":"Liu","year":"2015","journal-title":"J. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1182\/blood-2006-05-026039","article-title":"Cytokine and integrin stimulation synergize to promote higher levels of GATA-2, c-myb, and CD34 protein in primary human hematopoietic progenitors from bone marrow","volume":"109","author":"Dao","year":"2007","journal-title":"Blood"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.stem.2017.07.004","article-title":"Long-Term Engraftment of Primary Bone Marrow Stromal Cells Repairs Niche Damage and Improves Hematopoietic Stem Cell Transplantation","volume":"21","author":"Abbuehl","year":"2017","journal-title":"Cell Stem Cell"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4612","DOI":"10.1182\/blood.V92.12.4612.424k04_4612_4621","article-title":"Adhesion to fibronectin maintains regenerative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells","volume":"92","author":"Dao","year":"1998","journal-title":"Blood"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S1074-7613(02)00281-9","article-title":"Beta1 integrin is not essential for hematopoiesis but is necessary for the T cell-dependent IgM antibody response","volume":"16","author":"Brakebusch","year":"2002","journal-title":"Immunity"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00277-015-2550-5","article-title":"LPS-stimulated human bone marrow stroma cells support myeloid cell development and progenitor cell maintenance","volume":"95","author":"Ziegler","year":"2016","journal-title":"Ann. Hematol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5824","DOI":"10.4049\/jimmunol.1103253","article-title":"Cutting edge: LPS-induced emergency myelopoiesis depends on TLR4-expressing nonhematopoietic cells","volume":"188","author":"Boettcher","year":"2012","journal-title":"J. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1182\/blood-2014-04-570762","article-title":"Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis","volume":"124","author":"Boettcher","year":"2014","journal-title":"Blood"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1038\/leu.2014.68","article-title":"G-CSF regulates hematopoietic stem cell activity, in part, through activation of Toll-like receptor signaling","volume":"28","author":"Schuettpelz","year":"2014","journal-title":"Leukemia"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1084\/jem.20181074","article-title":"EDA fibronectin-TLR4 axis sustains megakaryocyte expansion and inflammation in bone marrow fibrosis","volume":"216","author":"Malara","year":"2019","journal-title":"J. Exp. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/ncb3346","article-title":"Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal","volume":"18","author":"Pietras","year":"2016","journal-title":"Nat. Cell Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1016\/S0301-472X(99)00084-3","article-title":"Role of abnormal integrin-cytoskeletal interactions in impaired beta1 integrin function in chronic myelogenous leukemia hematopoietic progenitors","volume":"27","author":"Bhatia","year":"1999","journal-title":"Exp. Hematol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"4667","DOI":"10.4049\/jimmunol.165.8.4667","article-title":"In vivo roles of integrins during leukocyte development and traffic: Insights from the analysis of mice chimeric for alpha 5, alpha v, and alpha 4 integrins","volume":"165","author":"Arroyo","year":"2000","journal-title":"J. Immunol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.stem.2014.01.007","article-title":"Conversion of danger signals into cytokine signals by hematopoietic stem and progenitor cells for regulation of stress-induced hematopoiesis","volume":"14","author":"Zhao","year":"2014","journal-title":"Cell Stem Cell"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"585","DOI":"10.3324\/haematol.2018.197210","article-title":"Pro-inflammatory cytokine blockade attenuates myeloid expansion in a murine model of rheumatoid arthritis","volume":"105","author":"Hernandez","year":"2020","journal-title":"Haematologica"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1182\/blood.V97.7.1975","article-title":"Cross-talk between alpha(4)beta(1)\/alpha(5)beta(1) and c-Kit results in opposing effect on growth and survival of hematopoietic cells via the activation of focal adhesion kinase, mitogen-activated protein kinase, and Akt signaling pathways","volume":"97","author":"Kapur","year":"2001","journal-title":"Blood"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1083\/jcb.200702080","article-title":"Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: Integrins in red cell development","volume":"177","author":"Eshghi","year":"2007","journal-title":"J. Cell Biol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.exphem.2014.01.007","article-title":"Stage-specific functional roles of integrins in murine erythropoiesis","volume":"42","author":"Ulyanova","year":"2014","journal-title":"Exp. Hematol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1182\/blood.2019004230","article-title":"Adhesion to fibronectin via alpha5beta1 integrin supports expansion of the megakaryocyte lineage in primary myelofibrosis","volume":"135","author":"Matsuura","year":"2020","journal-title":"Blood"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"24690","DOI":"10.1074\/jbc.M412979200","article-title":"Cellular fibronectin binds to lysyl oxidase with high affinity and is critical for its proteolytic activation","volume":"280","author":"Fogelgren","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"660","DOI":"10.3324\/haematol.2015.141283","article-title":"Bone marrow fibrosis in myelofibrosis: Pathogenesis, prognosis and targeted strategies","volume":"101","author":"Zahr","year":"2016","journal-title":"Haematologica"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/j.1365-2141.1989.tb04310.x","article-title":"Transforming growth factor-beta regulates growth as well as collagen and fibronectin synthesis of human marrow fibroblasts","volume":"72","author":"Kimura","year":"1989","journal-title":"Br. J. Haematol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1172\/JCI26674","article-title":"The molecular mechanisms that control thrombopoiesis","volume":"115","author":"Kaushansky","year":"2005","journal-title":"J. Clin. Investig."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/S0092-8674(00)81301-X","article-title":"Differential requirements for alpha4 integrins during fetal and adult hematopoiesis","volume":"85","author":"Arroyo","year":"1996","journal-title":"Cell"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1182\/blood-2008-04-151506","article-title":"Lymphoid precursors are directed to produce dendritic cells as a result of TLR9 ligation during herpes infection","volume":"112","author":"Welner","year":"2008","journal-title":"Blood"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"7006","DOI":"10.1074\/jbc.M109.027581","article-title":"Apoptotic death of hematopoietic tumor cells through potentiated and sustained adhesion to fibronectin via VLA-4","volume":"285","author":"Saito","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.exphem.2004.10.002","article-title":"Engagement of integrin alpha4beta1 enhances thrombopoietin-induced megakaryopoiesis","volume":"33","author":"Fox","year":"2005","journal-title":"Exp. Hematol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1002\/ajh.25230","article-title":"Primary myelofibrosis: 2019 update on diagnosis, risk-stratification and management","volume":"93","author":"Tefferi","year":"2018","journal-title":"Am. J. Hematol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Manshouri, T., Verstovsek, S., Harris, D.M., Veletic, I., Zhang, X., Post, S.M., Bueso-Ramos, C.E., and Estrov, Z. (2019). Primary myelofibrosis marrow-derived CD14+\/CD34\u2212 monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytes. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222912"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s13045-014-0092-2","article-title":"Activated fibronectin-secretory phenotype of mesenchymal stromal cells in pre-fibrotic myeloproliferative neoplasms","volume":"7","author":"Schneider","year":"2014","journal-title":"J. Hematol. Oncol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"a011247","DOI":"10.1101\/cshperspect.a011247","article-title":"Toll-like receptor signaling","volume":"5","author":"Lim","year":"2013","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.exphem.2010.09.011","article-title":"MicroRNA-146a disrupts hematopoietic differentiation and survival","volume":"39","author":"Starczynowski","year":"2011","journal-title":"Exp. Hematol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/cmi.2012.61","article-title":"TLR signaling in B-cell development and activation","volume":"10","author":"Hua","year":"2013","journal-title":"Cell Mol. Immunol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"836485","DOI":"10.1155\/2012\/836485","article-title":"The effects of TLR activation on T-cell development and differentiation","volume":"2012","author":"Jin","year":"2012","journal-title":"Clin. Dev. Immunol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1016\/j.vaccine.2012.01.081","article-title":"The extradomain A of fibronectin (EDA) combined with poly(I:C) enhances the immune response to HIV-1 p24 protein and the protection against recombinant Listeria monocytogenes-Gag infection in the mouse model","volume":"30","author":"Munoz","year":"2012","journal-title":"Vaccine"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1038\/nature04186","article-title":"VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche","volume":"438","author":"Kaplan","year":"2005","journal-title":"Nature"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1002\/stem.2381","article-title":"Brief Report: Alternative Splicing of Extra Domain A (EIIIA) of Fibronectin Plays a Tissue-Specific Role in Hematopoietic Homeostasis","volume":"34","author":"Malara","year":"2016","journal-title":"Stem Cells"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ydbio.2007.07.005","article-title":"Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin","volume":"311","author":"Astrof","year":"2007","journal-title":"Dev. Biol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/12\/2717\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:47:01Z","timestamp":1760179621000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/9\/12\/2717"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,18]]},"references-count":97,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["cells9122717"],"URL":"https:\/\/doi.org\/10.3390\/cells9122717","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,18]]}}}