{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T21:33:51Z","timestamp":1791754431447,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Children"],"abstract":"<jats:p>Background: Thrombospondin-1 (TSP-1) is an extracellular glycoprotein that mediates the differentiation of pulmonary endothelial cells and specialized stem cells into alveolar epithelial lineage-specific cells during the repair phase after lung injury. Since bronchopulmonary dysplasia (BPD) involves the inhibition of lung development with altered lung structure and vasculature, differential expression of the THBS-1 gene may impact lung development and pulmonary endothelial cell repair and have an important role in BPD. Methods: This prospective single-center cohort study included ELBW infants with and without BPD. DNA from buccal swabs underwent RT-PCR with TaqMan probes, and TSP-1 protein was measured in tracheal aspirates. Statistical analyses used Chi-square tests, Fisher\u2019s exact tests, Wilcoxon Rank Sum tests, and t-tests (p &lt; 0.05). Results: ELBW infants with BPD had significantly lower gestational ages and birth weights compared to those without BPD [25 (24,26) and 27 (25,28) weeks; median (IQR); p = 0.008] and [712 (155) and 820 (153) grams; mean (SD); p = 0.002], respectively. There were significant differences in the haplotype distributions of THBS1 variants rs2664139\/rs1478604 (p = 0.006) and THBS1 variants rs1478605\/rs1478604 (p = 0.008) between no-BPD and BPD groups. There were also significant differences in airway TSP-1 protein levels between moderate and severe BPD patients [(p = 0.02) (no BPD: 527 (114\u20131755); moderate BPD: 312 (262\u2013641); and severe BPD 211: (117\u2013352) ng\/dL; median (IQR)]. Conclusions: Although no individual variants differed, two THBS1 haplotypes and early TSP-1 airway expression varied by BPD severity, suggesting a role for TSP-1 in lung development and BPD pathogenesis in ELBW infants.<\/jats:p>","DOI":"10.3390\/children12040424","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T06:06:35Z","timestamp":1743141995000},"page":"424","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Thrombospondin-1 Airway Expression and Thrombospondin-1 Gene Variants Are Associated with Bronchopulmonary Dysplasia in Extremely Low-Birth-Weight Infants: A Pilot Study"],"prefix":"10.3390","volume":"12","author":[{"given":"Parvathy","family":"Krishnan","sequence":"first","affiliation":[{"name":"Division of Newborn Medicine, The Regional Neonatal Intensive Care Unit, Maria Fareri Children\u2019s Hospital at Westchester Medical Center, Valhalla, NY 10595, USA"},{"name":"Tufts Medicine Pediatrics, Division of Newborn Medicine, Boston, MA 02111, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Sampath","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Children\u2019s Hospital of Michigan, Detroit Medical Center, Detroit, MI 48201, USA"},{"name":"Division of Neonatology, Hutzel Women\u2019s Hospital, Detroit Medical Center, Detroit, MI 48201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van","family":"Trinh","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, New York Medical College, Valhalla, NY 10595, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance","family":"Parton","sequence":"additional","affiliation":[{"name":"Division of Newborn Medicine, The Regional Neonatal Intensive Care Unit, Maria Fareri Children\u2019s Hospital at Westchester Medical Center, Valhalla, NY 10595, USA"},{"name":"Department of Pediatrics, New York Medical College, Valhalla, NY 10595, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1038\/s41572-019-0127-7","article-title":"Bronchopulmonary dysplasia","volume":"5","author":"Goss","year":"2019","journal-title":"Nat. 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