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We also explored whether changes in leukocyte TL between ages 4 and 8 were associated with outcomes. Methods: Data were obtained from a population-based birth cohort and included 647 children with TL at age 4 and emotional and behavioral assessments at age 8, 673 with TL and outcomes at age 8, and 315 with TL measured at both ages. TL was determined using quantitative PCR on blood samples and converted into z-scores for analysis. Emotional and behavioral problems\u2014including internalizing, externalizing, and total difficulties\u2014were assessed using the Strengths and Difficulties Questionnaire. Regression models were conducted using zero-inflated and negative binomial, adjusting for sociodemographic and lifestyle covariates. Results: No statistically significant associations were observed between leukocyte TL at ages 4 or 8, or TL changes over this period, and emotional and behavioral outcomes at age 8. Conclusions: Although no significant associations were found, further longitudinal research is warranted to clarify the role of TL as a potential psychobiomarker of emotional and behavioral disorders in childhood.<\/jats:p>","DOI":"10.3390\/children12070875","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T09:08:44Z","timestamp":1751447324000},"page":"875","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Telomere Length and Emotional and Behavioral Problems in Children from the Prospective Birth Cohort INfancia y Medio Ambiente (INMA) Study"],"prefix":"10.3390","volume":"12","author":[{"given":"Irene","family":"Campos-S\u00e1nchez","sequence":"first","affiliation":[{"name":"InTeO Research Group, Miguel Hernandez University, 03550 San Juan de Alicante, 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