{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:05:31Z","timestamp":1788779131918,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T00:00:00Z","timestamp":1612483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Children"],"abstract":"<jats:p>Background: Children with autism spectrum disorder (ASD) show certain characteristics in visual attention. These may generate differences with non-autistic children in the integration of relevant social information to set the basis of communication. Reliable and objective measurement of these characteristics in a language learning context could contribute to a more accurate early diagnosis of ASD. Gaze following and pupil dilation are being studied as possible reliable measures of visual attention for the early detection of ASD. The eye-tracking methodology allows objective measurement of these biomarkers. The aim of this study is to determine whether measurements of gaze following and pupillary dilation in a linguistic interaction task are potential objective biomarkers for the early diagnosis of ASD. Method: A group of 20 children between 17 and 24 months of age, made up of 10 neurotypical children (NT) and 10 children with an increased likelihood of developing ASD were paired together according to chronological age. A human face on a monitor pronounced pseudowords associated with pseudo-objects. Gaze following and pupil dilation were registered during the task These measurements were captured using eye-tracking methodology. Results: Significant statistical differences were found in the time of gaze fixation on the human face and on the object, as well as in the number of gazes. Children with an increased possibility of developing ASD showed a slightly higher pupil dilation than NT children. However, this difference was not statistically significant. Nevertheless, their pupil dilation was uniform throughout the different periods of the task while NT participants showed greater dilation on hearing the pseudoword. Conclusions: The fixing and the duration of gaze, objectively measured by a Tobii eye-tracking system, could be considered as potential biomarkers for early detection of ASD. Additionally, pupil dilation measurement could reflect differential activation patterns during word processing in possible ASD toddlers and NT toddlers.<\/jats:p>","DOI":"10.3390\/children8020113","type":"journal-article","created":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T08:33:48Z","timestamp":1612514028000},"page":"113","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Gaze Following and Pupil Dilation as Early Diagnostic Markers of Autism in Toddlers"],"prefix":"10.3390","volume":"8","author":[{"given":"Raquel","family":"Camero","sequence":"first","affiliation":[{"name":"Department of Psychology, University of Oviedo, 33003 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-4667","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Psychology, University of Oviedo, 33003 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Gallego","sequence":"additional","affiliation":[{"name":"Department of Experimental Psychology, Cognitive Processes and Speech Therapy, Complutense University of Madrid, 28223 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Karmiloff, K., and Karmiloff-Smith, A. (2001). Pathways to Language. From Fetus to Adolescent, Harvard University Press.","DOI":"10.4159\/9780674039322"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1073\/pnas.1114783109","article-title":"Infants deploy selective attention to the mouth of a talking face when learning speech","volume":"109","author":"Lewkowicz","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1017\/S0305000914000725","article-title":"Attention to the mouth and gaze following in infancy predict language development","volume":"42","author":"Tenenbaum","year":"2015","journal-title":"J. Child Lang."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1111\/j.1467-7687.2009.00833.x","article-title":"Gaze behavior and affect at 6 months: Predicting clinical outcomes and language development in typically developing infants and infants at risk for autism","volume":"12","author":"Young","year":"2009","journal-title":"Dev. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.biopsych.2013.07.009","article-title":"Speech disturbs face scanning in 6-month-olds who develop autism spectrum disorder","volume":"75","author":"Shic","year":"2014","journal-title":"Biol. Psychiatry"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"D\u2019Souza, D., D\u2019Souza, H., Johnson, M.H., and Karmiloff-Smith, A. (2015). Concurrent relations between face scanning and language: A cross syndrome infant study. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0139319"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Howard, P.L., Zhang, L., and Benson, V. (2019). What can eye movements tell us about subtle cognitive processing differences in autism?. Vision, 3.","DOI":"10.3390\/vision3020022"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.biopsych.2012.11.022","article-title":"Decreased spontaneous attention to social scenes in 6-month-old infants later diagnosed with autism spectrum disorders","volume":"74","author":"Chawarska","year":"2013","journal-title":"Biol. Psychiatry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1002\/aur.1894","article-title":"Validation of eye\u2014Tracking measures of social attention as a potential biomarker for autism clinical trials","volume":"11","author":"Murias","year":"2018","journal-title":"Autism Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.jaac.2018.06.023","article-title":"Development and validation of objective and quantitative eye tracking-based measures of autism risk and symptom levels","volume":"57","author":"Frazier","year":"2018","journal-title":"J. Am. Acad. Child Adolesc. Psychiatry"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"De Vries, L., Fouquaet, I., Boets, B., Naulaers, G., and Steyaert, J. (2020). Autism spectrum disorder and pupillometry: A systematic review and meta-analysis. Neurosci. Biobehav. Rev.","DOI":"10.1016\/j.neubiorev.2020.09.032"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1038\/s41467-018-03985-4","article-title":"Enhanced pupillary light reflex in infancy is associated with autism diagnosis in toddlerhood","volume":"9","author":"Gliga","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nn1421","article-title":"Gaze fixation and the neural circuitry of face processing in autism","volume":"8","author":"Dalton","year":"2005","journal-title":"Nat. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1001\/archpsyc.59.9.809","article-title":"Visual fixation patterns during viewing of naturalistic social situations as predictors of social competence in individuals with autism","volume":"59","author":"Klin","year":"2002","journal-title":"Arch. Gen. Psychiatry"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.rasd.2008.01.004","article-title":"Young children with Autism Spectrum Disorder look differently at positive versus negative emotional faces","volume":"2","year":"2008","journal-title":"Res. Autism Spectr. Disord."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep08284","article-title":"Shorter spontaneous fixation durations in infants with later emerging autism","volume":"5","author":"Wass","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1007\/s10803-015-2495-5","article-title":"Eye-tracking measurements of language processing: Developmental differences in children at high risk for ASD","volume":"45","author":"Arunachalam","year":"2015","journal-title":"J. Autism Dev. Disord."},{"key":"ref_18","unstructured":"Bauman, M.L., and Kemper, T.L. (2005). The Vagus: A mediator of behavioral and physiological features associated with autism. The Neurobiology of Autism, The Johns Hopkins University Press. [2nd ed.]."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.biopsycho.2019.05.004","article-title":"Autonomic response in autism spectrum disorder: Relationship to social and cognitive functioning","volume":"145","author":"Patriquin","year":"2019","journal-title":"Biol. Psychol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1007\/s10803-013-1971-z","article-title":"A review of cardiac autonomic measures: Considerations for examination of physiological response in children with autism spectrum disorder","volume":"45","author":"Benevides","year":"2015","journal-title":"J. Autism Dev. Disord."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1111\/desc.12085","article-title":"From outcome prediction to action selection: Developmental change in the role of action-effect bindings","volume":"16","author":"Verschoor","year":"2013","journal-title":"Dev. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1080\/13803390500376790","article-title":"Visual scanning and pupillary responses in young children with Autism Spectrum Disorder","volume":"28","author":"Anderson","year":"2006","journal-title":"J. Clin. Exp. Neuropsychol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1002\/dev.20352","article-title":"Larger tonic pupil size in young children with autism spectrum disorder","volume":"51","author":"Anderson","year":"2009","journal-title":"Dev. Psychobiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.mehy.2017.01.015","article-title":"Infant acetylcholine, dopamine, and melatonin dysregulation: Neonatal biomarkers and causal factors for ASD and ADHD phenotypes","volume":"100","author":"Hellmer","year":"2017","journal-title":"Med. Hypotheses."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.jpsychires.2011.01.008","article-title":"Can pupil size and pupil responses during visual scanning contribute to the diagnosis of autism spectrum disorder in children?","volume":"45","author":"Martineau","year":"2011","journal-title":"J. Psychiatr. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"398","DOI":"10.3389\/fpsyg.2016.00398","article-title":"The timing and effort of lexical access in natural and degraded speech","volume":"7","author":"Wagner","year":"2016","journal-title":"Front. Psychol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1037\/dev0001090","article-title":"A pupillometry study of multisensory social and linguistic processing in autism and typical development","volume":"56","author":"Segers","year":"2020","journal-title":"Dev. Psychol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Moore, M., Evans, V., Hanvey, G., and Johnson, C. (2017). Assessment of sleep in children with Autism Spectrum Disorder. Children, 4.","DOI":"10.3390\/children4080072"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1007\/s10803-018-3581-2","article-title":"Prevalence and characteristics of autism spectrum disorder among Spanish school-age children","volume":"48","author":"Voltas","year":"2018","journal-title":"J. Autism Dev. Disord."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.3389\/fpsyg.2018.02248","article-title":"Pupillary measures of the cognitive effort in auditory novel word processing and short-term retention","volume":"9","author":"Karousou","year":"2018","journal-title":"Front. Psychol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"252","DOI":"10.3758\/CABN.10.2.252","article-title":"Pupil diameter tracks changes in control state predicted by the adaptive gain theory of locus coeruleus function","volume":"10","author":"Gilzenrat","year":"2010","journal-title":"Cognit. Affect. Behav. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1469-8986.2004.00147.x","article-title":"Attention allocation in the dual-task paradigm as measured through behavioral and psychophysiological responses","volume":"41","author":"Karatekin","year":"2004","journal-title":"Psychophysiology"},{"key":"ref_33","unstructured":"Robins, D.L., Fein, D., and Barton, M. (2009). The Modified Checklist for Autism in Toddlers, Revised, with Follow-Up (M-CHAT-R\/F), Self-Published."},{"key":"ref_34","unstructured":"Josse, D., and Pereda, S. (1997). Brunet L\u00e9zine Revisado: Escala de Desarrollo Psicomotor de la Primera Infancia (Brunet L\u00e9zine Reviewed: A Psychomothor Development Scale for Infants), Symtec."},{"key":"ref_35","unstructured":"Lord, C., Rutter, M., DiLavore, P.C., Risi, S., Gotham, K., and Bishop, S.L. (2012). Autism Diagnostic Observation Schedule, Second Edition (ADOS-2) Manual (Part I): Modules 1\u20134, Western Psychological Services."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"53","DOI":"10.5209\/rlog.58684","article-title":"La imitaci\u00f3n como herramienta para investigar y evaluar el desarrollo ling\u00fc\u00edstico temprano: Un estudio piloto de repetici\u00f3n de palabras y pseudopalabras (Imitation as a tool for research and assessment of early language development: A pilot study of word and non-word repetition)","volume":"3","author":"Mariscal","year":"2013","journal-title":"Rev. Investig. Logop."},{"key":"ref_37","unstructured":"Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences, Lawrence Earlbaum Associates. [2nd ed.]."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1017\/S1366728916000651","article-title":"Pupil dilation is sensitive to the cognate status of words: Further evidence for non-selectivity in bilingual lexical access","volume":"20","author":"Guasch","year":"2017","journal-title":"Bilingualism"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.biopsych.2019.05.006","article-title":"Joint attention in infancy and the emergence of autism","volume":"86","author":"Thorup","year":"2019","journal-title":"Biol. Psychiatry"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4012","DOI":"10.1016\/j.neuropsychologia.2010.10.015","article-title":"Sound before meaning: Word learning in autistic disorders","volume":"48","author":"Norbury","year":"2010","journal-title":"Neuropsychologia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1007\/s10803-016-2716-6","article-title":"Factors affecting age at ASD diagnosis in UK: No evidence that diagnosis age has decreased between 2004 and 2014","volume":"46","author":"Brett","year":"2016","journal-title":"J. Autism Dev. Disord."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1467-8624.2011.01692.x","article-title":"Individual differences in lexical processing at 18 months predict vocabulary growth in typically developing and late-talking toddlers","volume":"83","author":"Fernald","year":"2012","journal-title":"Child Dev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4412","DOI":"10.1007\/s10803-020-04493-5","article-title":"Exploring social biomarkers in high-functioning adults with autism and Asperger\u2019s versus healthy controls: A cross-sectional analysis","volume":"50","author":"Hollander","year":"2020","journal-title":"J. Autism Dev. Disord."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1007\/s10803-009-0767-7","article-title":"Abnormal transient pupillary light reflex in individuals with autism spectrum disorders","volume":"39","author":"Fan","year":"2009","journal-title":"J. Autism Dev. Disord."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1002\/aur.1745","article-title":"Atypical pupillary light reflex in 2\u20136-year-old children with autism spectrum disorders","volume":"10","author":"Dinalankara","year":"2017","journal-title":"Autism Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/s41598-020-58254-6","article-title":"A longitudinal study of pupillary light reflex in 6- to 24-month children","volume":"10","author":"Kercher","year":"2020","journal-title":"Sci. Rep."}],"container-title":["Children"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2227-9067\/8\/2\/113\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:19:59Z","timestamp":1760159999000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2227-9067\/8\/2\/113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,5]]},"references-count":46,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["children8020113"],"URL":"https:\/\/doi.org\/10.3390\/children8020113","relation":{"has-preprint":[{"id-type":"doi","id":"10.20944\/preprints202012.0388.v1","asserted-by":"object"}]},"ISSN":["2227-9067"],"issn-type":[{"value":"2227-9067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,5]]}}}