{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T05:48:32Z","timestamp":1768456112076,"version":"3.49.0"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T00:00:00Z","timestamp":1641686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>A retrospective case control study was undertaken at the molecular biology department of a private center for reproductive medicine in order to determine whether any correlation exists between mitochondrial DNA (mtDNA) content of cleavage-stage preimplantation embryos and their developmental potential. A total of 69 couples underwent IVF treatment (averaged women age: 36.5, SD 4.9) and produced a total of 314 embryos. A single blastomere was biopsied from each embryo at the cleavage stage (day-3 post-fertilization) subjected to low-pass next generation sequencing (NGS), for the purpose of detecting aneuploidy. For each sample, the number of mtDNA reads obtained after analysis using NGS was divided by the number of reads attributable to the nuclear genome. The mtDNA copy number amount was found to be higher in aneuploid embryos than in those that were euploid (mean mtDNA ratio \u00b1 SD: 6.3 \u00b1 7.5 versus 7.1 \u00b1 5.8, p &lt; 0.004; U Mann\u2013Whitney test), whereas no statistically significant differences in mtDNA content were seen in relation to embryo morphology (6.6 \u00b1 4.8 vs. 8.5 \u00b1 13.6, p 0.09), sex (6.6 \u00b1 4.1 vs. 6.2 \u00b1 6.8, p 0.16), maternal age (6.9 \u00b1 7.8 vs. 6.7 \u00b1 4.5, p 0.14) or its ability to implant (7.4 \u00b1 6.6 vs. 5.1 \u00b1 4.6, p 0.18). The mtDNA content cannot serve as a useful biomarker at this point in development. However, further studies investigating both quantitative and qualitative aspects of mtDNA are still required to fully evaluate the relationship between mitochondrial DNA and human reproduction.<\/jats:p>","DOI":"10.3390\/cimb44010020","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T20:29:26Z","timestamp":1641760166000},"page":"273-287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos\u2014Impact on Infertility Outcome"],"prefix":"10.3390","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1942-5522","authenticated-orcid":false,"given":"Amira","family":"Podolak","sequence":"first","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9199-5212","authenticated-orcid":false,"given":"Joanna","family":"Liss","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"},{"name":"Department of Medical Biology and Genetics, University of Gdansk, 80-308 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2738-8952","authenticated-orcid":false,"given":"Jolanta","family":"Kiewisz","sequence":"additional","affiliation":[{"name":"Department of Human Histology and Embryology, Medical Faculty, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Pukszta","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celina","family":"Cybulska","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Rychlowski","sequence":"additional","affiliation":[{"name":"Laboratory of Virus Molecular Biology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aron","family":"Lukaszuk","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"},{"name":"The Center of Postgraduate Medical Education, 1st Department of Obstetrics and Gynecology, University of Gdansk, 01-004 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6094-2273","authenticated-orcid":false,"given":"Grzegorz","family":"Jakiel","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"},{"name":"The Center of Postgraduate Medical Education, 1st Department of Obstetrics and Gynecology, University of Gdansk, 01-004 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Lukaszuk","sequence":"additional","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"},{"name":"Department of Obstetrics and Gynecology Nursing, Medical University of Gdansk, 80-210 Gdansk, Poland"},{"name":"iYoni App by LifeBite, 10-763 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,9]]},"reference":[{"key":"ref_1","unstructured":"(2021, October 26). 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