{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:53:59Z","timestamp":1780408439494,"version":"3.54.1"},"reference-count":77,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education and Science of the Russian Federation","award":["075-15-2021-933"],"award-info":[{"award-number":["075-15-2021-933"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying low-abundance proteins in human chorionic villi using the proposed \u201c1DE-gel concentration\u201d method. This involves the removal of SDS in a short electrophoresis run in a stacking gel without protein separation. Following the in-gel digestion of the obtained holistic single protein band, we used the peptide mixture for further LC\u2013MS\/MS analysis. Statistically significant results were derived from six datasets, containing three treatments, each from two tissue sources (elective or missed abortions). The 1DE-gel concentration increased the coverage of the chorionic villus proteome. Our approach allowed the identification of 15 low-abundance proteins, of which some had not been previously detected via the mass spectrometry of trophoblasts. In the post hoc data analysis, we found a dubious or uncertain protein (PSG7) encoded on human chromosome 19 according to neXtProt. A proteomic sample preparation workflow with the 1DE-gel concentration can be used as a prospective tool for uncovering the low-abundance part of the human proteome.<\/jats:p>","DOI":"10.3390\/cimb44050140","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T21:23:27Z","timestamp":1652131407000},"page":"2069-2088","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi"],"prefix":"10.3390","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4448-7629","authenticated-orcid":false,"given":"Timur","family":"Shkrigunov","sequence":"first","affiliation":[{"name":"Center of Scientific and Practical Education, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1843-7668","authenticated-orcid":false,"given":"Pavel","family":"Pogodin","sequence":"additional","affiliation":[{"name":"Laboratory of Structure-Function Based Drug Design, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4532-4274","authenticated-orcid":false,"given":"Victor","family":"Zgoda","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Biology, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olesya","family":"Larina","sequence":"additional","affiliation":[{"name":"Laboratory of Microsomal Oxidation, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia","family":"Kisrieva","sequence":"additional","affiliation":[{"name":"Laboratory of Microsomal Oxidation, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Klimenko","sequence":"additional","affiliation":[{"name":"Center for Family Planning and Reproduction, Moscow Department of Health, 117209 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg","family":"Latyshkevich","sequence":"additional","affiliation":[{"name":"Center for Family Planning and Reproduction, Moscow Department of Health, 117209 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Klimenko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Lisitsa","sequence":"additional","affiliation":[{"name":"Center of Scientific and Practical Education, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-4607","authenticated-orcid":false,"given":"Natalia","family":"Petushkova","sequence":"additional","affiliation":[{"name":"Laboratory of Microsomal Oxidation, Institute of Biomedical Chemistry, 119121 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/prca.201000144","article-title":"Utility of formalin-fixed, paraffin-embedded liver biopsy specimens for global proteomic analysis in nonalcoholic steatohepatitis","volume":"5","author":"Bell","year":"2011","journal-title":"Proteom. Clin. Appl."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.drudis.2017.11.011","article-title":"Understanding missing proteins: A functional perspective","volume":"23","author":"Zhou","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1089\/omi.2015.0035","article-title":"The quest of the human proteome and the missing proteins: Digging deeper","volume":"19","author":"Reddy","year":"2015","journal-title":"Omics J. Integr. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jprot.2016.08.005","article-title":"Why are they missing?: Bioinformatics characterization of missing human proteins","volume":"149","author":"Elguoshy","year":"2016","journal-title":"J. Proteom."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1038\/labinvest.3780334","article-title":"Characterization of human villous and extravillous trophoblasts isolated from first trimester placenta","volume":"81","author":"Tarrade","year":"2001","journal-title":"Lab. Investig."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1146\/annurev.publhealth.26.021304.144351","article-title":"Abortion in the United States","volume":"26","author":"Harper","year":"2005","journal-title":"Annu. Rev. Public Health"},{"key":"ref_7","unstructured":"(2022, May 05). The Human Protein Atlas: Tissue Atlas, Placenta-Specific Proteome. Available online: https:\/\/www.proteinatlas.org\/humanproteome\/tissue\/placenta."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1095\/biolreprod.106.056127","article-title":"The cytotrophoblast layer of human chorionic villi becomes thinner but maintains its structural integrity during gestation","volume":"76","author":"Mori","year":"2007","journal-title":"Biol. Reprod."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.pbiomolbio.2013.11.007","article-title":"An integrative view on the physiology of human early placental villi","volume":"114","author":"Huppertz","year":"2014","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/978-1-4939-2694-7_8","article-title":"Solubilization of proteins: The importance of lysis buffer choice","volume":"1312","author":"Peach","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_11","first-page":"111","article-title":"Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis","volume":"355","author":"Rabilloud","year":"2007","journal-title":"Methods Mol. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wang, F., Wang, L., Xu, Z., and Liang, G. (2013). Identification and analysis of multi-protein complexes in placenta. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062988"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.placenta.2017.11.001","article-title":"Proteomics and bioinformatics analysis of altered protein expression in the placental villous tissue from early recurrent miscarriage patients","volume":"61","author":"Pan","year":"2018","journal-title":"Placenta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.placenta.2020.09.013","article-title":"Optimization of label-free nano LC-MS\/MS analysis of the placental proteome","volume":"101","author":"Luyten","year":"2020","journal-title":"Placenta"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12014-021-09324-y","article-title":"Proteome profiling of human placenta reveals developmental stage-dependent alterations in protein signature","volume":"18","author":"Shojaeian","year":"2021","journal-title":"Clin. Proteom."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1194\/jlr.R900005-JLR200","article-title":"Thematic review series: Proteomics. Proteomic analysis of lipid-protein complexes","volume":"50","author":"Vaisar","year":"2009","journal-title":"J. Lipid Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12953-016-0098-5","article-title":"Removal of SDS from biological protein digests for proteomic analysis by mass spectrometry","volume":"14","author":"Ilavenil","year":"2016","journal-title":"Proteome Sci."},{"key":"ref_18","unstructured":"Meena, S., and Naik, M. (2019). Chapter 4\u2014Plant proteomics: A guide to improve the proteome coverage. Advances in Biological Science Research, Academic Press."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ymeth.2011.05.005","article-title":"The importance of the digest: Proteolysis and absolute quantification in proteomics","volume":"54","author":"Brownridge","year":"2011","journal-title":"Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1159\/000366371","article-title":"Comparative proteomic profile of the human placenta in normal and fetal growth restriction subjects","volume":"34","author":"Miao","year":"2014","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1700011","DOI":"10.1002\/pmic.201700011","article-title":"Sample Clean-up Strategies for ESI Mass Spectrometry Applications in Bottom-up Proteomics: Trends from 2012 to 2016","volume":"17","author":"Tubaon","year":"2017","journal-title":"Proteomics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"272","DOI":"10.12688\/f1000research.2-272.v1","article-title":"The beauty of being (label)-free: Sample preparation methods for SWATH-MS and next-generation targeted proteomics","volume":"2","author":"Vowinckel","year":"2013","journal-title":"F1000Research"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1021\/acs.jproteome.6b00199","article-title":"Automated SDS Depletion for Mass Spectrometry of Intact Membrane Proteins though Transmembrane Electrophoresis","volume":"15","author":"Kachuk","year":"2016","journal-title":"J. Proteome Res."},{"key":"ref_24","first-page":"17","article-title":"SDS polyacrylamide gel electrophoresis for N-terminal protein sequencing","volume":"64","author":"Smith","year":"1997","journal-title":"Methods Mol. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e93","DOI":"10.1002\/cpps.93","article-title":"Proteome Analysis Using Gel-LC-MS\/MS","volume":"96","author":"Goldman","year":"2019","journal-title":"Curr. Protoc. Protein Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Gomis-Cebolla, J., Scaramal Ricietto, A., and Ferr\u00e9, J. (2018). A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal. Toxins, 10.","DOI":"10.3390\/toxins10050193"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"695","DOI":"10.2144\/000112020","article-title":"Concentration and isolation of DNA from biological fluids by agarose gel isotachophoresis","volume":"39","author":"Kondratova","year":"2005","journal-title":"BioTechniques"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4324987","DOI":"10.1155\/2016\/4324987","article-title":"Optimization of Urea Based Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue for Shotgun Proteomics","volume":"2016","author":"Luebker","year":"2016","journal-title":"Int. J. Proteom."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"770","DOI":"10.3346\/jkms.2015.30.6.770","article-title":"The Proteomic Analysis of Human Placenta with Pre-eclampsia and Normal Pregnancy","volume":"30","author":"Yang","year":"2015","journal-title":"J. Korean Med. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"458748","DOI":"10.1155\/2010\/458748","article-title":"Differential proteome analysis of the preeclamptic placenta using optimized protein extraction","volume":"2010","author":"Centlow","year":"2010","journal-title":"J. Biotechnol. Biomed."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"647143","DOI":"10.1155\/2014\/647143","article-title":"Quantitative proteomics analysis of altered protein expression in the placental villous tissue of early pregnancy loss using isobaric tandem mass tags","volume":"2014","author":"Ni","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of Protein Using Bicinchoninic Acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels","volume":"68","author":"Shevchenko","year":"1996","journal-title":"Anal. Chem."},{"key":"ref_35","first-page":"40","article-title":"Optimization of liquid chromatography with mass spectrometric detection method for the qualitative and semi-quantitative proteomic analysis","volume":"3","author":"Rodchenkova","year":"2013","journal-title":"Analitika"},{"key":"ref_36","unstructured":"(2022, May 05). ProteoWizard Home Page. Available online: https:\/\/proteowizard.sourceforge.io."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1002\/pmic.201000595","article-title":"SearchGUI: An open-source graphical user interface for simultaneous OMSSA and X!Tandem searches","volume":"11","author":"Vaudel","year":"2011","journal-title":"Proteomics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/nbt.3109","article-title":"PeptideShaker enables reanalysis of MS-derived proteomics data sets","volume":"33","author":"Vaudel","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_39","unstructured":"(2022, May 05). The Contaminant Repository for Affinity Purification (CRAPome) Database. Available online: https:\/\/reprint-apms.org\/?q=chooseworkflow."},{"key":"ref_40","unstructured":"(2022, May 05). The R Project for Statistical Computing. Available online: https:\/\/www.r-project.org."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.21105\/joss.01686","article-title":"Welcome to the Tidyverse","volume":"4","author":"Wickham","year":"2019","journal-title":"J. Open Source Softw."},{"key":"ref_42","unstructured":"(2022, May 05). Ggupset: Combination Matrix Axis for \u2018ggplot2\u2019 to Create \u2018UpSet\u2019 Plots. Available online: https:\/\/CRAN.R-project.org\/package=ggupset."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3718","DOI":"10.1093\/bioinformatics\/btv428","article-title":"dendextend: An R package for visualizing, adjusting and comparing trees of hierarchical clustering","volume":"31","author":"Galili","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1007\/s43032-020-00330-3","article-title":"Gross morphologic appearance of chorionic villi and the risk of chromosomal abnormalities","volume":"28","author":"Sobhani","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_45","unstructured":"Sadler, T. (2012). Langman\u2019s Medical Embryology, Lippincott Williams and Wilkins. [12th ed.]."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1021\/pr900262z","article-title":"Application of slicing of one-dimensional gels with subsequent slice-by-slice mass spectrometry for the proteomic profiling of human liver cytochromes P450","volume":"9","author":"Lisitsa","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1038\/s41598-017-02599-y","article-title":"Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research","volume":"7","author":"Vergauwen","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_48","unstructured":"(2022, May 05). Spinning Around: Tips and Tricks for Using Centrifugal Filters. Available online: https:\/\/bitesizebio.com\/44738\/spinning-around-tips-and-tricks-for-using-centrifugal-filters\/."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/978-1-62703-360-2_17","article-title":"Label-free quantitative shotgun proteomics using normalized spectral abundance factors","volume":"1002","author":"Neilson","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1002\/rcm.7829","article-title":"Comparative evaluation of label-free quantification methods for shotgun proteomics","volume":"31","author":"Bubis","year":"2017","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1074\/mcp.RA119.001714","article-title":"Reproducibility, Specificity and Accuracy of Relative Quantification Using Spectral Library-based Data-independent Acquisition","volume":"19","author":"Barkovits","year":"2020","journal-title":"Mol. Cell Proteom."},{"key":"ref_52","unstructured":"(2022, May 05). Female Pregnancy GO Annotations (GO:0007565). Available online: https:\/\/www.ebi.ac.uk\/QuickGO\/annotations?agoUsage=descendants&goUsageRelationships=is_a,part_of,occurs_in&goId=GO:0007565."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1109\/TVCG.2014.2346248","article-title":"UpSet: Visualization of Intersecting Sets","volume":"20","author":"Lex","year":"2014","journal-title":"IEEE Trans. Vis. Comput. Graph."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"113","DOI":"10.2307\/3614533","article-title":"A note on the historical development of logic diagrams: Leibniz, Euler and Venn","volume":"53","author":"Baron","year":"1969","journal-title":"Math. Gaz."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"bbab108","DOI":"10.1093\/bib\/bbab108","article-title":"Venn diagrams in bioinformatics","volume":"22","author":"Jia","year":"2021","journal-title":"Brief. Bioinform."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Finocchi, I., and Petreschi, R. (2001, January 5\u20136). Hierarchical Clustering of Trees: Algorithms and Experiments. Proceedings of the Third Intranational Workshop Conference: Algorithm Engineering and Experimentation, Washington, DC, USA.","DOI":"10.1007\/3-540-44808-X_9"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1093\/molbev\/msy196","article-title":"Tanglegrams Are Misleading for Visual Evaluation of Tree Congruence","volume":"36","year":"2019","journal-title":"Mol. Biol. Evol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/jee\/39.2.269","article-title":"Individual comparisons of grouped data by ranking methods","volume":"39","author":"Wilcoxon","year":"1946","journal-title":"J. Econ. Entomol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1021\/pr401144x","article-title":"Metrics for the Human Proteome Project 2013\u20132014 and strategies for finding missing proteins","volume":"13","author":"Lane","year":"2014","journal-title":"J. Proteome Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Fagerberg","year":"2015","journal-title":"Science"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/rda.13747","article-title":"Activity of selected glycosidases and availability of their substrates in bovine placenta during pregnancy and parturition with and without retained foetal membranes","volume":"55","author":"Wawrzykowski","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1095\/biolreprod.114.124248","article-title":"Dynamic proteomic profiles of in vivo- and in vitro-produced mouse postimplantation extraembryonic tissues and placentas","volume":"91","author":"Sui","year":"2014","journal-title":"Biol. Reprod."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/11056","article-title":"Cooperation between mDia1 and ROCK in Rho-induced actin reorganization","volume":"1","author":"Watanabe","year":"1999","journal-title":"Nat. Cell Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.ydbio.2007.07.041","article-title":"Cartilage link protein 1 (Crtl1), an extracellular matrix component playing an important role in heart development","volume":"310","author":"Wirrig","year":"2007","journal-title":"Dev. Biol."},{"key":"ref_65","unstructured":"(2022, May 05). An Online Catalog of Human Genes and Genetic Disorders: Pregnancy-Specific Beta-1-Glycoprotein 11; PSG11. Available online: https:\/\/omim.org\/entry\/176401."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1006\/scbi.1998.0119","article-title":"The carcinoembryonic antigen (CEA) family: Structures, suggested functions and expression in normal and malignant tissues","volume":"9","year":"1999","journal-title":"Semin. Cancer Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0140-6736(77)91135-7","article-title":"Concentrations of pregnancy-specific beta 1-glycoprotein in maternal blood in normal pregnancy and in intrauterine growth retardation","volume":"1","author":"Gordon","year":"1977","journal-title":"Lancet"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1515\/jpme.1981.9.2.67","article-title":"Radioimmunoassay of SP1 (pregnancy-specific beta1-glycoprotein) in maternal blood and in amniotic fluid normal and pathologic pregnancies","volume":"9","author":"Geiger","year":"1981","journal-title":"J. Perinat. Med."},{"key":"ref_69","unstructured":"(2022, May 05). The Carcinoembryonic Antigen Homepage: Human CEA Family. Available online: http:\/\/carcinoembryonic-antigen.de\/human."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Rattila, S., Dunk, C., Im, M., Grichenko, O., Zhou, Y., Yanez-Mo, M., Blois, S., Yamada, K., Erez, O., and Gomez-Lopez, N. (2019). Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin A5\u03b21 Is a Modulator of Extravillous Trophoblast Functions. Cells, 8.","DOI":"10.3390\/cells8111369"},{"key":"ref_71","unstructured":"(2022, May 05). neXtProt: A Knowledge Platform for Human Proteins, Putative Pregnancy-Specific Beta-1-Glycoprotein 7 (PSBG-7). Available online: https:\/\/www.nextprot.org\/entry\/NX_Q13046\/."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.jprot.2016.08.002","article-title":"In-depth analysis of protein inference algorithms using multiple search engines and well-defined metrics","volume":"150","author":"Audain","year":"2017","journal-title":"J. Proteom."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1021\/acs.jproteome.7b00640","article-title":"IdentiPy: An Extensible Search Engine for Protein Identification in Shotgun Proteomics","volume":"17","author":"Levitsky","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1134\/S0026893319010096","article-title":"Comparative Analysis of the Performance of Mascot and IdentiPy Algorithms on a Benchmark Dataset Obtained by Tandem Mass Spectrometry Analysis of Testicular Biopsies","volume":"53","author":"Lisitsa","year":"2019","journal-title":"Mol. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4959","DOI":"10.1021\/acs.jproteome.5b00578","article-title":"Combination of Multiple Spectral Libraries Improves the Current Search Methods Used to Identify Missing Proteins in the Chromosome-Centric Human Proteome Project","volume":"14","author":"Cho","year":"2015","journal-title":"J. Proteome Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3389\/fgene.2020.00027","article-title":"Identification of the Prognosis-Related lncRNAs and Genes in Gastric Cancer","volume":"11","author":"Su","year":"2020","journal-title":"Front. Genet."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/0006-291X(90)91696-P","article-title":"Identification of a new carcinoembryonic antigen (CEA) family member in human fetal liver\u2014cloning and sequence determination of pregnancy-specific glycoprotein 7","volume":"168","author":"Khan","year":"1990","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/44\/5\/140\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:08:01Z","timestamp":1760137681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/44\/5\/140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,9]]},"references-count":77,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["cimb44050140"],"URL":"https:\/\/doi.org\/10.3390\/cimb44050140","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,9]]}}}