{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:32:47Z","timestamp":1785295967045,"version":"3.55.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005668","name":"FAPDF","doi-asserted-by":"publisher","award":["193.001.668\/2017"],"award-info":[{"award-number":["193.001.668\/2017"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"FAPDF","doi-asserted-by":"publisher","award":["CNPq-PAPES VI 421923\/2017-2"],"award-info":[{"award-number":["CNPq-PAPES VI 421923\/2017-2"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fiocruz\/CNPq","award":["193.001.668\/2017"],"award-info":[{"award-number":["193.001.668\/2017"]}]},{"name":"Fiocruz\/CNPq","award":["CNPq-PAPES VI 421923\/2017-2"],"award-info":[{"award-number":["CNPq-PAPES VI 421923\/2017-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Mycobacterium bovis BCG is the only vaccine against tuberculosis. The variable forms of cultivation throughout the years, before seed-lots were developed, allowed in vitro evolution of the original strain, generating a family of vaccines with different phenotypic and genotypic characteristics. Molecular studies revealed regions of difference (RDs) in the genomes of the various BCG strains. This work aims to characterize the gene pair rv3407-rv3408 (vapB47-vapC47), coding for a toxin\u2013antitoxin system of the VapBC family, and to evaluate possible transcriptional effects due to the adjacent BCG Moreau-specific genomic deletion RD16. We show that these genes are co-transcribed in BCG strains Moreau and Pasteur, and that the inactivation of an upstream transcriptional repressor (Rv3405c) due to RD16 has a polar effect, leading to increased vapBC47 expression. Furthermore, we detect VapB47 DNA binding in vitro, dependent on a 5\u2032 vapB47 sequence that contributes to a palindrome, spanning the promoter and coding region. Our data shed light on the regulation of VapBC systems and on the impact of the BCG Moreau RD16 deletion in the expression of adjacent genes, contributing to a better understanding of BCG Moreau physiology.<\/jats:p>","DOI":"10.3390\/cimb45080412","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T12:45:53Z","timestamp":1691498753000},"page":"6538-6549","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Genomic Deletion RD16 on the Expression of the Mycobacterium bovis BCG Moreau VapBC47 Toxin-Antitoxin System"],"prefix":"10.3390","volume":"45","author":[{"given":"Talita Duarte","family":"Pagani","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma Rezende","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane","family":"Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1721-0608","authenticated-orcid":false,"given":"Leonardo Henrique Ferreira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6858-0860","authenticated-orcid":false,"given":"Marcos Gustavo Araujo","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teca Calcagno","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bacteriologia, Centro de Refer\u00eancia Prof. H\u00e9lio Fraga, Escola Nacional de Sa\u00fade P\u00fablica Sergio Arouca, Fiocruz, Rio de Janeiro 21041-210, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim Maurits","family":"Degrave","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6251-7342","authenticated-orcid":false,"given":"Napole\u00e3o Fonseca","family":"Valadares","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular, Universidade de Bras\u00edlia, Bras\u00edlia 70904-970, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-5083","authenticated-orcid":false,"given":"Leila","family":"Mendon\u00e7a-Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00f4mica Funcional e Bioinform\u00e1tica, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref_1","unstructured":"(2021). 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