{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:15:57Z","timestamp":1790961357607,"version":"4.1.0"},"reference-count":77,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education (MOE)","award":["2022RIS-005"],"award-info":[{"award-number":["2022RIS-005"]}]},{"name":"Ministry of Education (MOE)","award":["2021R1G1A1091690"],"award-info":[{"award-number":["2021R1G1A1091690"]}]},{"name":"Korean Government (MIST)","award":["2022RIS-005"],"award-info":[{"award-number":["2022RIS-005"]}]},{"name":"Korean Government (MIST)","award":["2021R1G1A1091690"],"award-info":[{"award-number":["2021R1G1A1091690"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Melatonin, a hormone secreted by the pineal gland of vertebrates, regulates sleep, blood pressure, and circadian and seasonal rhythms, and acts as an antioxidant and anti-inflammatory agent. We investigated the protective effects of melatonin against markers of D-galactose (D-Gal)-induced hepatocellular aging, including liver inflammation, hepatocyte structural damage, and non-alcoholic fatty liver. Mice were divided into four groups: phosphate-buffered saline (PBS, control), D-Gal (200 mg\/kg\/day), melatonin (20 mg\/kg), and D-Gal (200 mg\/kg) and melatonin (20 mg) cotreatment. The treatments were administered once daily for eight consecutive weeks. Melatonin treatment alleviated D-Gal-induced hepatocyte impairment. The AST level was significantly increased in the D-Gal-treated groups compared to that in the control group, while the ALT level was decreased compared to the melatonin and D-Gal cotreated group. Inflammatory genes, such as IL1-\u03b2, NF-\u03baB, IL-6, TNF\u03b1, and iNOS, were significantly increased in the D-Gal aging model, whereas the expression levels of these genes were low in the D-Gal and melatonin cotreated group. Interestingly, the expression levels of hepatic steatosis-related genes, such as LXR\u03b1, C\/EBP\u03b1, PPAR\u03b1, ACC, ACOX1, and CPT-1, were markedly decreased in the D-Gal and melatonin cotreated group. These results suggest that melatonin suppresses hepatic steatosis and inflammation in a mouse model of D-Gal-induced aging.<\/jats:p>","DOI":"10.3390\/cimb45100530","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T08:25:09Z","timestamp":1697531109000},"page":"8412-8426","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effects of Melatonin on Liver of D-Galactose-Induced Aged Mouse Model"],"prefix":"10.3390","volume":"45","author":[{"given":"Ran","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Republic of Korea"},{"name":"Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju-si 26339, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Yong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4589-2052","authenticated-orcid":false,"given":"Hyun-Jung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju-si 26339, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1038\/s41392-022-01251-0","article-title":"Aging and aging-related diseases: From molecular mechanisms to interventions and treatments","volume":"7","author":"Guo","year":"2022","journal-title":"Signal Transduct. 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