{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T09:51:24Z","timestamp":1782294684315,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries, Korea","doi-asserted-by":"publisher","award":["20210656"],"award-info":[{"award-number":["20210656"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries, Korea","doi-asserted-by":"publisher","award":["RS-2023-00251456"],"award-info":[{"award-number":["RS-2023-00251456"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation, Korea","doi-asserted-by":"publisher","award":["20210656"],"award-info":[{"award-number":["20210656"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation, Korea","doi-asserted-by":"publisher","award":["RS-2023-00251456"],"award-info":[{"award-number":["RS-2023-00251456"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Focal cerebral ischemia (fCI) can result in brain injury and sensorimotor deficits. Brown algae are currently garnering scientific attention as potential therapeutic candidates for fCI. This study investigated the therapeutic effects of the hot water extract of Petalonia binghamiae (wPB), a brown alga, in in vitro and in vivo models of fCI. The neuroprotective efficacy of wPB was evaluated in an in vitro excitotoxicity model established using HT-22 cells challenged with glutamate. Afterward, C57\/BL6 mice were administered wPB for 7 days (10 or 100 mg\/kg, intragastric) and subjected to middle cerebral artery occlusion and reperfusion (MCAO\/R) operation, which was used as an in vivo fCI model. wPB co-incubation significantly inhibited cell death, oxidative stress, and apoptosis, as well as stimulated the expression of heme oxygenase-1 (HO-1), an antioxidant enzyme, and the nuclear translocation of its upstream regulator, nuclear factor erythroid 2-related factor 2 (Nrf2) in HT-22 cells challenged with glutamate-induced excitotoxicity. Pretreatment with either dose of wPB significantly attenuated infarction volume, neuronal death, and sensorimotor deficits in an in vivo fCI model. Furthermore, the attenuation of oxidative stress and apoptosis in the ischemic lesion accompanied the wPB-associated protection. This study suggests that wPB can counteract fCI via an antioxidative effect, upregulating the Nrf2\/HO-1 pathway.<\/jats:p>","DOI":"10.3390\/cimb45100531","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T10:43:10Z","timestamp":1697539390000},"page":"8427-8443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Neuroprotective Effects of Water Extract from Brown Algae Petalonia binghamiae in an Experimental Model of Focal Cerebral Ischemia In Vitro and In Vivo"],"prefix":"10.3390","volume":"45","author":[{"given":"Sun Ho","family":"Eom","sequence":"first","affiliation":[{"name":"Healinnols Inc., Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6680-5379","authenticated-orcid":false,"given":"Geum-Lan","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Bae","family":"Kang","sequence":"additional","affiliation":[{"name":"Healinnols Inc., Daejeon 34054, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nam-Seob","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6655-7061","authenticated-orcid":false,"given":"Do Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Gil","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung Choon","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bong Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Technology, Hanbat National University, Daejeon 34158, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6355-5738","authenticated-orcid":false,"given":"Ju-Young","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Histology & Institute of Veterinary Science, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6843-0360","authenticated-orcid":false,"given":"Dong-Sub","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Natural Product Research, Korea Prime Pharmacy Co., Ltd., Gwangju 61473, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7055-6341","authenticated-orcid":false,"given":"Seung Yun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/JNN.0b013e3181ce5c70","article-title":"Gender and stroke symptoms: A review of the current literature","volume":"42","author":"Beal","year":"2010","journal-title":"J. Neurosci. Nurs."},{"key":"ref_2","first-page":"1","article-title":"Deaths: Leading causes for 2004","volume":"56","author":"Heron","year":"2007","journal-title":"Natl. Vital. Stat. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.tips.2008.02.003","article-title":"Beyond NMDA and AMPA glutamate receptors: Emerging mechanisms for ionic imbalance and cell death in stroke","volume":"29","author":"Besancon","year":"2008","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_4","unstructured":"Ramon, R., and Julio C\u00e9sar, G. (2016). Ischemic Stroke, IntechOpen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4253","DOI":"10.1523\/JNEUROSCI.0211-07.2007","article-title":"Selective dysfunction of hippocampal CA1 astrocytes contributes to delayed neuronal damage after transient forebrain ischemia","volume":"27","author":"Ouyang","year":"2007","journal-title":"J. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1002\/glia.20985","article-title":"Astrocyte targeted overexpression of Hsp72 or SOD2 reduces neuronal vulnerability to forebrain ischemia","volume":"58","author":"Xu","year":"2010","journal-title":"Glia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.1212\/WNL.0b013e3182697e70","article-title":"Blood-brain barrier, reperfusion injury, and hemorrhagic transformation in acute ischemic stroke","volume":"79","author":"Khatri","year":"2012","journal-title":"Neurology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10277","DOI":"10.1523\/JNEUROSCI.18-24-10277.1998","article-title":"Mitochondrial control of acute glutamate excitotoxicity in cultured cerebellar granule cells","volume":"18","author":"Castilho","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4246","DOI":"10.2174\/1381612826666200708133912","article-title":"Pathophysiology of Ischemic Stroke: Role of Oxidative Stress","volume":"26","author":"Rojas","year":"2020","journal-title":"Curr. Pharm. Des."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1007\/s10072-017-2938-1","article-title":"Pathogenic mechanisms following ischemic stroke","volume":"38","author":"Khoshnam","year":"2017","journal-title":"Neurol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4356386","DOI":"10.1155\/2020\/4356386","article-title":"Oxidative Stress-Mediated Blood-Brain Barrier (BBB) Disruption in Neurological Diseases","volume":"2020","author":"Song","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.neuroscience.2008.07.007","article-title":"NF-kappaB signaling in cerebral ischemia","volume":"158","author":"Ridder","year":"2009","journal-title":"Neuroscience"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"10262","DOI":"10.1523\/JNEUROSCI.2818-05.2005","article-title":"Apoptosis-inducing factor triggered by poly(ADP-ribose) polymerase and Bid mediates neuronal cell death after oxygen-glucose deprivation and focal cerebral ischemia","volume":"25","author":"Culmsee","year":"2005","journal-title":"J. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100124","DOI":"10.1016\/j.mtbio.2021.100124","article-title":"Reactive-oxygen-species-scavenging nanomaterials for resolving inflammation","volume":"11","author":"Huang","year":"2021","journal-title":"Mater. Today Bio."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"30657","DOI":"10.1021\/acsomega.2c02675","article-title":"Emerging ROS-Modulating Technologies for Augmentation of the Wound Healing Process","volume":"7","author":"Polaka","year":"2022","journal-title":"ACS Omega"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1124\/pr.120.019422","article-title":"On the Clinical Pharmacology of Reactive Oxygen Species","volume":"72","author":"Casas","year":"2020","journal-title":"Pharmacol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"956792","DOI":"10.1155\/2013\/956792","article-title":"Achieving the balance between ROS and antioxidants: When to use the synthetic antioxidants","volume":"2013","author":"Poljsak","year":"2013","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_18","first-page":"426","article-title":"Can antioxidants be effective therapeutics? Curr Opin Investig Drugs","volume":"11","author":"Murphy","year":"2010","journal-title":"Curr. Opin. Investig. Drugs"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"832957","DOI":"10.3389\/fmars.2022.832957","article-title":"Novel Bioactive Compounds from Marine Sources as a Tool for Functional Food Development","volume":"9","author":"Ghosh","year":"2022","journal-title":"Front. Mar. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"667764","DOI":"10.3389\/fmars.2021.667764","article-title":"Marine Organisms as a Rich Source of Biologically Active Peptides","volume":"8","author":"Macedo","year":"2021","journal-title":"Front. Mar. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Catanesi, M., Caioni, G., Castelli, V., Benedetti, E., Angelo, M., and Cimini, A. (2021). Benefits under the Sea: The Role of Marine Compounds in Neurodegenerative Disorders. Mar. Drugs, 19.","DOI":"10.3390\/md19010024"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4530","DOI":"10.1128\/AEM.02479-07","article-title":"Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by \"helper\" heterotrophic bacteria","volume":"74","author":"Morris","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.copbio.2010.01.015","article-title":"Approaches for novel enzyme discovery from marine environments","volume":"21","author":"Lee","year":"2010","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.ijbiomac.2018.04.110","article-title":"Antibacterial macro molecules from marine organisms","volume":"115","author":"Nalini","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Khalifa, S.A.M., Elias, N., Farag, M.A., Chen, L., Saeed, A., Hegazy, M.E., Moustafa, M., Washed, A.A., Mousawi, S.M., and Musharraf, S. (2019). Marine Natural Products: A Source of Novel Anticancer Drugs. Mar. Drugs, 17.","DOI":"10.3390\/md17090491"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.3390\/md8102619","article-title":"Antiviral lead compounds from marine sponges","volume":"8","author":"Sagar","year":"2010","journal-title":"Mar. Drugs"},{"key":"ref_27","unstructured":"Buchanan, J. (2007). The Crustose Brown Algae of New Zealand: A Taxonomic Study. [Master\u2019s Thesis, Victoria University of Wellington]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.bbrc.2008.06.015","article-title":"Petalonia improves glucose homeostasis in streptozotocin-induced diabetic mice","volume":"373","author":"Kang","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_29","first-page":"754","article-title":"Anti-inflammatory Effect of Petalonia binghamiae in LPS- Induced Macrophages is Mediated by Suppression of iNOS and COX-2","volume":"12","author":"Yang","year":"2010","journal-title":"Int. J. Agric. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.3390\/md13052666","article-title":"The Cytoprotective Effect of Petalonia binghamiae Methanol Extract against Oxidative Stress in C2C12 Myoblasts: Mediation by Upregulation of Heme Oxygenase-1 and Nuclear Factor-Erythroid 2 Related Factor 2","volume":"13","author":"Kang","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3390\/cimb43010030","article-title":"Water Extract of Mixed Mushroom Mycelia Grown on a Solid Barley Medium Is Protective against Experimental Focal Cerebral Ischemia","volume":"43","author":"Jeong","year":"2021","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_32","first-page":"H1262","article-title":"Mechanisms of cerebral vasodilation by superoxide, hydrogen peroxide, and peroxynitrite","volume":"271","author":"Wei","year":"1996","journal-title":"Am. J. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1747-4949.2009.00387.x","article-title":"Oxidative stress and its role in the pathogenesis of ischaemic stroke","volume":"4","author":"Allen","year":"2009","journal-title":"Int. J. Stroke"},{"key":"ref_34","unstructured":"Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Institute for Laboratory Animal Research, and Division on Earth and Life Studies (2011). Guide for the Care and Use of Laboratory Animals, The National Academies Press."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Lee, J., Jeong, J., Jeong, Y.-G., Kim, D.-K., Lee, N.S., Na, C.S., Doh, E.S., and Han, S.Y. (2019). Platycarya strobilacea leaf extract protects mice brain with focal cerebral ischemia by antioxidative property. Anat. Cell Biol., 52.","DOI":"10.5115\/acb.19.141"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.freeradbiomed.2005.05.003","article-title":"Antioxidant strategies in the treatment of stroke","volume":"39","author":"Margaill","year":"2005","journal-title":"Free Radic. Biol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1007\/s12035-016-9707-7","article-title":"Nrf2 Weaves an Elaborate Network of Neuroprotection Against Stroke","volume":"54","author":"Jiang","year":"2017","journal-title":"Mol. Neurobiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10495-021-01661-5","article-title":"New insights into targeting mitochondria in ischemic injury","volume":"26","author":"Jia","year":"2021","journal-title":"Apoptosis"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0304-3770(03)00033-0","article-title":"Morphology and life history studies of Endarachne binghamiae (Scytosiphonaceae, Phaeophyta) from the Azores","volume":"76","author":"Parente","year":"2003","journal-title":"Aquat. Bot."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s40495-014-0013-7","article-title":"MicroRNAs: New players in cancer prevention targeting Nrf2, oxidative stress and inflammatory pathways","volume":"1","author":"Zhang","year":"2015","journal-title":"Curr. Pharmacol. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1196\/annals.1427.036","article-title":"The Nrf2-ARE pathway: An indicator and modulator of oxidative stress in neurodegeneration","volume":"1147","author":"Johnson","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1089\/ars.2010.3573","article-title":"Regulation of neuronal oxidative and nitrosative stress by endogenous protective pathways and disease processes","volume":"14","author":"Hardingham","year":"2011","journal-title":"Antioxid. Redox Signal."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.freeradbiomed.2009.07.035","article-title":"Nrf2:INrf2 (Keap1) signaling in oxidative stress","volume":"47","author":"Kaspar","year":"2009","journal-title":"Free Radic. Biol. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"116","DOI":"10.4103\/0973-1296.96553","article-title":"Hepatoprotective and antioxidant activity of standardized herbal extracts","volume":"8","author":"Hiraganahalli","year":"2012","journal-title":"Pharmacogn. Mag."},{"key":"ref_45","unstructured":"Ambreen Bano, A.H., and Swati Sharma Snober, S. (2023). Mir. Exploring Poisonous Plants Medicinal Values, Toxicity Responses, and Therapeutic Uses, CRC Press."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"115271","DOI":"10.1016\/j.jep.2022.115271","article-title":"A review of the ethnomedicinal uses, chemistry, and pharmacological properties of the genus Acanthus (Acanthaceae)","volume":"293","author":"Matos","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4037","DOI":"10.2174\/0929867323666160627104453","article-title":"Synthesis and Bioactivity of (R)-Ricinoleic Acid Derivatives: A Review","volume":"23","author":"Pabis","year":"2016","journal-title":"Curr. Med. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s002100100427","article-title":"Pro- and anti-inflammatory actions of ricinoleic acid: Similarities and differences with capsaicin","volume":"364","author":"Vieira","year":"2001","journal-title":"Naunyn Schmiedebergs Arch. Pharmacol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1007\/s10753-016-0416-1","article-title":"Protective Role of Liriodendrin in Sepsis-Induced Acute Lung Injury","volume":"39","author":"Yang","year":"2016","journal-title":"Inflammation"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1139\/cjpp-2015-0120","article-title":"Fucoidan reduces inflammatory response in a rat model of hepatic ischemia-reperfusion injury","volume":"93","author":"Li","year":"2015","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"100250","DOI":"10.1016\/j.phyplu.2022.100250","article-title":"Fucoidan alleviates the mitochondria and endoplasmic reticulum stresses in ischemic rat livers","volume":"2","author":"Slim","year":"2022","journal-title":"Phytomedicine Plus"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Park, J.H., Ahn, J.H., Lee, T.K., Par, C.W., Kim, B., Lee, J.-C., Kim, D.W., Shin, M.G., Cho, J.H., and Lee, C.-H. (2020). Laminarin Pretreatment Provides Neuroprotection against Forebrain Ischemia\/Reperfusion Injury by Reducing Oxidative Stress and Neuroinflammation in Aged Gerbils. Mar. Drugs, 18.","DOI":"10.3390\/md18040213"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.arr.2016.09.006","article-title":"Dysfunction of the neurovascular unit in ischemic stroke and neurodegenerative diseases: An aging effect","volume":"34","author":"Cai","year":"2017","journal-title":"Ageing Res. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kugler, E.C., Greenwood, J., and MacDonald, R.B. (2021). The \u201cNeuro-Glial-Vascular\u201d Unit: The Role of Glia in Neurovascular Unit Formation and Dysfunction. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.732820"}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/45\/10\/531\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:08:39Z","timestamp":1760130519000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/45\/10\/531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,17]]},"references-count":54,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["cimb45100531"],"URL":"https:\/\/doi.org\/10.3390\/cimb45100531","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,17]]}}}