{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T22:25:06Z","timestamp":1784586306158,"version":"3.55.0"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2022-301"],"award-info":[{"award-number":["075-15-2022-301"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Adult-onset Still\u2019s disease (AOSD) is a complex systemic inflammatory disorder, categorized as an \u2018IL-1 driven\u2019 inflammasomapathy. Despite this, the interaction between T and B cells remains poorly understood. We conducted a study, enrolling 7 patients with relapsing AOSD and 15 healthy control subjects, utilizing deep flow cytometry analysis to examine peripheral blood T- and B-cell subsets. T-cell and B-cell subsets were significantly altered in patients with AOSD. Within CD4+ T cells, Th2 cells were decreased. Additionally, Th17 cell and follicular Th cell subsets were altered within CD45RA\u2013CD62L+ and CD45RA\u2013CD62L\u2013 Th cells in patients with AOSD compared to healthy controls. We identified changes in CD8+ T cell maturation and \u2018polarization\u2019 in AOSD patients, with an elevated presence of the TEMRA CD8+ T cell subset. Furthermore, the percentage of Tc1 cells was decreased, while the frequency of CCR6\u2013CXCR3\u2013 Tc2 cells was elevated. Finally, we determined that the frequency of CD5+CD27\u2013 B cells was dramatically decreased in patients with AOSD compared to healthy controls. Further investigations on a large group of patients with AOSD are required to evaluate these adaptive immunity cells in the disease pathogenesis.<\/jats:p>","DOI":"10.3390\/cimb46020075","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T11:25:55Z","timestamp":1706786755000},"page":"1177-1191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Deep Immunophenotyping of Circulating T and B Cells in Relapsing Adult-Onset Still\u2019s Disease"],"prefix":"10.3390","volume":"46","author":[{"given":"Valentina","family":"Myachikova","sequence":"first","affiliation":[{"name":"Rheumatology and Immunopathology Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"},{"name":"Autoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Kudryavtsev","sequence":"additional","affiliation":[{"name":"Autoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"},{"name":"Laboratory of Cellular Immunology, Institute of Experimental Medicine, 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8493-5211","authenticated-orcid":false,"given":"Artem","family":"Rubinstein","sequence":"additional","affiliation":[{"name":"Autoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"},{"name":"Laboratory of Cellular Immunology, Institute of Experimental Medicine, 197376 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-7184","authenticated-orcid":false,"given":"Arthur","family":"Aquino","sequence":"additional","affiliation":[{"name":"Autoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Isakov","sequence":"additional","affiliation":[{"name":"Department of Immunology, First St. Petersburg State Medical University, 197022 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7577-628X","authenticated-orcid":false,"given":"Alexey","family":"Golovkin","sequence":"additional","affiliation":[{"name":"Autoimmune and Autoinflammatory Diseases Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Maslyanskiy","sequence":"additional","affiliation":[{"name":"Rheumatology and Immunopathology Research Laboratory, Federal State Budgetary Institution \u201cAlmazov National Medical Research Centre\u201d of the Ministry of Health of the Russian Federation, 197341 St. Petersburg, Russia"},{"name":"Scientific, Clinical and Educational Centre of Gastroenterology and Hepatology, Saint Petersburg State University, 199034 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1136\/adc.16.87.156","article-title":"On a Form of Chronic Joint Disease in Children","volume":"16","author":"Still","year":"1941","journal-title":"Arch. Dis. Child."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1136\/ard.30.2.121","article-title":"Still\u2019s Disease in the Adult","volume":"30","author":"Bywaters","year":"1971","journal-title":"Ann. Rheum. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/s12969-015-0047-3","article-title":"Gene-expression Analysis of Adult-Onset Still\u2019s Disease and Systemic Juvenile Idiopathic Arthritis is Consistent with a Continuum of a Single disease Entity","volume":"13","author":"Nirmala","year":"2015","journal-title":"Pediatr. Rheumatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.autrev.2014.01.058","article-title":"Adult-Onset Still\u2019s Disease","volume":"13","author":"Jamilloux","year":"2014","journal-title":"Autoimmun. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Jia, J., Wang, M., Ma, Y., Meng, J., Zhu, D., Chen, X., Shi, H., Sun, Y., Liu, H., and Cheng, X. (2023). Neutrophil Extracellular Trap-Induced Intermediate Monocytes Trigger Macrophage Activation Syndrome in Adult-Onset Still\u2019s Disease. BMC Med., 21.","DOI":"10.1186\/s12916-023-03231-9"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.clim.2016.05.010","article-title":"Cytokine Profile in Adult-Onset Still\u2019s Disease: Comparison with Systemic Juvenile Idiopathic Arthritis","volume":"169","author":"Inoue","year":"2016","journal-title":"Clin. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ruscitti, P., Cantarini, L., Nigrovic, P.A., McGonagle, D., and Giacomelli, R. (2024). Recent Advances and Evolving Concepts in Still\u2019s Disease. Nat. Rev. Rheumatol., ahead of print.","DOI":"10.1038\/s41584-023-01065-6"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/s41584-018-0081-x","article-title":"Mechanisms, Biomarkers and Targets for Adult-Onset Still\u2019s Disease","volume":"14","author":"Feist","year":"2018","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.3899\/jrheum.161354","article-title":"Elevated Expression of the NLRP3 Inflammasome and Its Correlation with Disease Activity in Adult-Onset Still Disease","volume":"44","author":"Hsieh","year":"2017","journal-title":"J. Rheumatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1111\/nep.12785","article-title":"The NLRP3 Inflammasome in Kidney Disease and Autoimmunity","volume":"21","author":"Hutton","year":"2016","journal-title":"Nephrol. Carlton Vic."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1101\/sqb.2013.78.021246","article-title":"IL-1\u03b2 Strikingly Enhances Antigen-Driven CD4 and CD8 T-Cell Responses. Cold Spring Harb","volume":"78","author":"Wang","year":"2013","journal-title":"Symp. Quant. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bent, R., Moll, L., Grabbe, S., and Bros, M. (2018). Interleukin-1 Beta-A Friend or Foe in Malignancies?. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082155"},{"key":"ref_13","first-page":"424","article-title":"Preliminary Criteria for Classification of Adult Still\u2019s Disease","volume":"19","author":"Yamaguchi","year":"1992","journal-title":"J. Rheumatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.3899\/jrheum.100247","article-title":"Clinical Manifestations but Not Cytokine Profiles Differentiate Adult-Onset Still\u2019s Disease and Sepsis","volume":"37","author":"Rau","year":"2010","journal-title":"J. Rheumatol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Golovkin, A., Kalinina, O., Bezrukikh, V., Aquino, A., Zaikova, E., Karonova, T., Melnik, O., Vasilieva, E., and Kudryavtsev, I. (2021). Imbalanced Immune Response of T-Cell and B-Cell Subsets in Patients with Moderate and Severe COVID-19. Viruses, 13.","DOI":"10.3390\/v13101966"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kudryavtsev, I.V., Arsentieva, N.A., Korobova, Z.R., Isakov, D.V., Rubinstein, A.A., Batsunov, O.K., Khamitova, I.V., Kuznetsova, R.N., Savin, T.V., and Akisheva, T.V. (2022). Heterogenous CD8+ T Cell Maturation and \u201cPolarization\u201d in Acute and Convalescent COVID-19 Patients. Viruses, 14.","DOI":"10.3390\/v14091906"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s13075-021-02532-9","article-title":"The Heterogeneous Human Memory CCR6+ T Helper-17 Populations Differ in T-Bet and Cytokine Expression but All Activate Synovial Fibroblasts in an IFN\u03b3-Independent Manner","volume":"23","author":"Dankers","year":"2021","journal-title":"Arthritis Res. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kotake, S., Yago, T., Kobashigawa, T., and Nanke, Y. (2017). The Plasticity of Th17 Cells in the Pathogenesis of Rheumatoid Arthritis. J. Clin. Med., 6.","DOI":"10.3390\/jcm6070067"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.immuni.2010.12.012","article-title":"Human Blood CXCR5(+)CD4(+) T Cells Are Counterparts of T Follicular Cells and Contain Specific Subsets That Differentially Support Antibody Secretion","volume":"34","author":"Morita","year":"2011","journal-title":"Immunity"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.3389\/fimmu.2018.01707","article-title":"T Follicular Helper-Like Cells in Inflamed Non-Lymphoid Tissues","volume":"9","author":"Hutloff","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"6357","DOI":"10.1038\/s41467-020-19002-6","article-title":"SLAMF7 and IL-6R Define Distinct Cytotoxic versus Helper Memory CD8+ T Cells","volume":"11","author":"Loyal","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8604","DOI":"10.1038\/s41598-021-88013-0","article-title":"Analysis of Peripheral Inflammatory T Cell Subsets and Their Effector Function in Patients with Birdshot Retinochoroiditis","volume":"11","author":"Trombke","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3610","DOI":"10.4049\/jimmunol.167.7.3610","article-title":"Bm1-Bm5 Classification of Peripheral Blood B Cells Reveals Circulating Germinal Center Founder Cells in Healthy Individuals and Disturbance in the B Cell Subpopulations in Patients with Primary Sj\u00f6gren\u2019s Syndrome","volume":"167","author":"Bohnhorst","year":"2001","journal-title":"J. Immunol."},{"key":"ref_24","first-page":"2189","article-title":"Proinflammatory Cytokine Profiles in Sera and Pathological Tissues of Patients with Active Untreated Adult Onset Still\u2019s Disease","volume":"31","author":"Chen","year":"2004","journal-title":"J. Rheumatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/s12016-019-08747-8","article-title":"Autoinflammatory Pathogenesis and Targeted Therapy for Adult-Onset Still\u2019s Disease","volume":"58","author":"Li","year":"2020","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1007\/s40265-018-0956-9","article-title":"Adult-Onset Still\u2019s Disease: Molecular Pathophysiology and Therapeutic Advances","volume":"78","author":"Sfriso","year":"2018","journal-title":"Drugs"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1093\/rheumatology\/keq284","article-title":"Potential Role of Th17 Cells in the Pathogenesis of Adult-Onset Still\u2019s Disease","volume":"49","author":"Chen","year":"2010","journal-title":"Rheumatol. Oxf. Engl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1186\/s13075-015-0773-4","article-title":"Association of CXCL10 and CXCL13 Levels with Disease Activity and Cutaneous Manifestation in Active Adult-Onset Still\u2019s Disease","volume":"17","author":"Han","year":"2015","journal-title":"Arthritis Res. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"46652","DOI":"10.1038\/srep46652","article-title":"Elevated Circulating Levels of the Interferon-\u03b3-Induced Chemokines Are Associated with Disease Activity and Cutaneous Manifestations in Adult-Onset Still\u2019s Disease","volume":"7","author":"Han","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.clim.2004.03.023","article-title":"Adult Still\u2019s Disease Reflects a Th2 Rather than a Th1 Cytokine Profile","volume":"112","author":"Saiki","year":"2004","journal-title":"Clin. Immunol. Orlando Fla."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1136\/ard.2003.013680","article-title":"Predominance of Th1 Cytokine in Peripheral Blood and Pathological Tissues of Patients with Active Untreated Adult Onset Still\u2019s Disease","volume":"63","author":"Chen","year":"2004","journal-title":"Ann. Rheum. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1093\/rheumatology\/keac439","article-title":"AOSD Endotypes Based on Immune Cell Profiles: Patient Stratification with Hierarchical Clustering Analysis","volume":"62","author":"Guo","year":"2023","journal-title":"Rheumatol. Oxf. Engl."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2058738418791284","DOI":"10.1177\/2058738418791284","article-title":"Characteristic Patterns of HLA Presentation and T Cell Differentiation in Adult-Onset Still\u2019s Disease","volume":"32","author":"Jung","year":"2018","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e787","DOI":"10.1097\/MD.0000000000000787","article-title":"The Pathologic Findings of Skin, Lymph Node, Liver, and Bone Marrow in Patients with Adult-Onset Still Disease","volume":"94","author":"Kim","year":"2015","journal-title":"Medicine"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chi, H., Hong, X., Dai, N., Chen, L., Zhang, H., Liu, H., Cheng, X., Ye, J., Shi, H., and Hu, Q. (2023). The Landscape of Innate and Adaptive Immune Cell Subsets in Patients with Adult-Onset Still\u2019s Disease. Rheumatol. Oxf. Engl., kead507.","DOI":"10.1093\/rheumatology\/kead507"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13000-021-01159-3","article-title":"Adult-Onset Still\u2019s Disease with Multiple Lymphadenopathy: A Case Report and Literature Review","volume":"16","author":"Huang","year":"2021","journal-title":"Diagn. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jdermsci.2010.05.004","article-title":"Granzyme B Is a Novel Interleukin-18 Converting Enzyme","volume":"59","author":"Omoto","year":"2010","journal-title":"J. Dermatol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s00403-013-1382-1","article-title":"CD8+ T Cell Granzyme B Activates Keratinocyte Endogenous IL-18","volume":"306","author":"Akeda","year":"2014","journal-title":"Arch. Dermatol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.4049\/jimmunol.130.6.2708","article-title":"The Role of Interleukin 1 in Human B Cell Activation: Inhibition of B Cell Proliferation and the Generation of Immunoglobulin-Secreting Cells by an Antibody against Human Leukocytic Pyrogen","volume":"130","author":"Lipsky","year":"1983","journal-title":"J. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s13075-023-03070-2","article-title":"B Cell Subsets in Adult-Onset Still\u2019s Disease: Potential Candidates for Disease Pathogenesis and Immunophenotyping","volume":"25","author":"Fang","year":"2023","journal-title":"Arthritis Res. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1084\/jem.168.6.1979","article-title":"Monoreactive High Affinity and Polyreactive Low Affinity Rheumatoid Factors Are Produced by CD5+ B Cells from Patients with Rheumatoid Arthritis","volume":"168","author":"Burastero","year":"1988","journal-title":"J. Exp. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1002\/art.1780310509","article-title":"B Cells Expressing CD5 Are Increased in Sj\u00f6gren\u2019s Syndrome","volume":"31","author":"Tovar","year":"1988","journal-title":"Arthritis Rheum."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1146\/annurev.iy.11.040193.002441","article-title":"Origin of Murine B Cell Lineages","volume":"11","author":"Kantor","year":"1993","journal-title":"Annu. Rev. Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1038\/ni833","article-title":"B Cells Regulate Autoimmunity by Provision of IL-10","volume":"3","author":"Fillatreau","year":"2002","journal-title":"Nat. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4390","DOI":"10.1182\/blood-2004-11-4284","article-title":"Identification and Characterization of Circulating Human Transitional B Cells","volume":"105","author":"Sims","year":"2005","journal-title":"Blood"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/j.jaci.2015.09.014","article-title":"In-Depth Characterization of CD24(High)CD38(High) Transitional Human B Cells Reveals Different Regulatory Profiles","volume":"137","author":"Simon","year":"2016","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1136\/annrheumdis-2016-210021","article-title":"A New Autoinflammatory and Autoimmune Syndrome Associated with NLRP1 Mutations: NAIAD (NLRP1-Associated Autoinflammation with Arthritis and Dyskeratosis)","volume":"76","author":"Grandemange","year":"2017","journal-title":"Ann. Rheum. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13075-016-1212-x","article-title":"Early Changes in Gene Expression and Inflammatory Proteins in Systemic Juvenile Idiopathic Arthritis Patients on Canakinumab Therapy","volume":"19","author":"Brachat","year":"2017","journal-title":"Arthritis Res. Ther."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Ghannam, K., Zernicke, J., Kedor, C., Listing, J., Burmester, G.R., Foell, D., and Feist, E. (2021). Distinct Effects of Interleukin-1\u03b2 Inhibition upon Cytokine Profile in Patients with Adult-Onset Still\u2019s Disease and Active Articular Manifestation Responding to Canakinumab. J. Clin. Med., 10.","DOI":"10.3390\/jcm10194400"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1002\/art.21061","article-title":"Rapid Responses to Anakinra in Patients with Refractory Adult-Onset Still\u2019s Disease","volume":"52","author":"Fitzgerald","year":"2005","journal-title":"Arthritis Rheum."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.semarthrit.2007.04.002","article-title":"Anakinra in Patients with Treatment-Resistant Adult-Onset Still\u2019s Disease: Four Case Reports with Serial Cytokine Measurements and a Review of the Literature","volume":"37","author":"Wacker","year":"2007","journal-title":"Semin. Arthritis Rheum."}],"container-title":["Current Issues in Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1467-3045\/46\/2\/75\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:52:51Z","timestamp":1760104371000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1467-3045\/46\/2\/75"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,1]]},"references-count":51,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["cimb46020075"],"URL":"https:\/\/doi.org\/10.3390\/cimb46020075","relation":{},"ISSN":["1467-3045"],"issn-type":[{"value":"1467-3045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,1]]}}}