{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:07:10Z","timestamp":1767841630954,"version":"3.49.0"},"reference-count":23,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korean Nephrology Research Foundation","award":["FMC research fund 2023"],"award-info":[{"award-number":["FMC research fund 2023"]}]},{"name":"Korean Nephrology Research Foundation","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}]},{"name":"Korean Nephrology Research Foundation","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}]},{"name":"Korean Nephrology Research Foundation","award":["RS-2023-00274788"],"award-info":[{"award-number":["RS-2023-00274788"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["FMC research fund 2023"],"award-info":[{"award-number":["FMC research fund 2023"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["RS-2023-00274788"],"award-info":[{"award-number":["RS-2023-00274788"]}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["FMC research fund 2023"],"award-info":[{"award-number":["FMC research fund 2023"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["RS-2023-00274788"],"award-info":[{"award-number":["RS-2023-00274788"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["FMC research fund 2023"],"award-info":[{"award-number":["FMC research fund 2023"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["HR22C1832"],"award-info":[{"award-number":["HR22C1832"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2021R1I1A3047973"],"award-info":[{"award-number":["2021R1I1A3047973"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS-2023-00274788"],"award-info":[{"award-number":["RS-2023-00274788"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Dual immunoglobulin domain-containing cell adhesion molecule (DICAM) is a type I transmembrane protein that presents in various cells including renal tubular cells. This study evaluated the expression and protective role of DICAM in renal tubular cell injury. HK-2 cells were incubated and treated with lipopolysaccharide (LPS, 30 \u03bcg\/mL) or hydrogen peroxide (H2O2, 100 \u03bcM) for 24 h. To investigate the effect of the gene silencing of DICAM, small interfering RNA of DICAM was used. Additionally, to explain its role in cellular response to injury, DICAM was overexpressed using an adenoviral vector. DICAM protein expression levels significantly increased following treatment with LPS or H2O2 in HK-2 cells. In response to oxidative stress, DICAM showed an earlier increase (2\u20134 h following treatment) than neutrophil gelatinase-associated lipocalin (NGAL) (24 h following treatment). DICAM gene silencing increased the protein expression of inflammation-related markers, including IL-1\u03b2, TNF-\u03b1, NOX4, integrin \u03b21, and integrin \u03b23, in H2O2-induced HK-2 cell injury. Likewise, in the LPS-induced HK-2 cell injury, DICAM knockdown led to a decrease in occludin levels and an increase in integrin \u03b23, IL-1\u03b2, and IL-6 levels. Furthermore, DICAM overexpression followed by LPS-induced HK-2 cell injury resulted in an increase in occludin levels and a decrease in integrin \u03b21, integrin \u03b23, TNF-\u03b1, IL-1\u03b2, and IL-6 levels, suggesting an alleviating effect on inflammatory responses. DICAM was elevated in the early stage of regular tubular cell injury and may protect against renal tubular injury through its anti-inflammatory properties. DICAM has a potential as an early diagnostic marker and therapeutic target for renal cell injury.<\/jats:p>","DOI":"10.3390\/cimb46030115","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T11:31:21Z","timestamp":1708947081000},"page":"1757-1767","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dual Immunoglobulin Domain-Containing Cell Adhesion Molecule Increases Early in Renal Tubular Cell Injury and Plays Anti-Inflammatory Role"],"prefix":"10.3390","volume":"46","author":[{"given":"Jin","family":"Han","sequence":"first","affiliation":[{"name":"Laboratory for Arthritis and Cartilage Biology, Research Institute of Aging and Metabolism, Kyungpook National University, Daegu 41404, Republic of Korea"},{"name":"Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju-Min","family":"Yook","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se-Hyun","family":"Oh","sequence":"additional","affiliation":[{"name":"Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu Kyung","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee-Yeon","family":"Jung","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-3665","authenticated-orcid":false,"given":"Ji-Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7031-5214","authenticated-orcid":false,"given":"Jang-Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0953-3343","authenticated-orcid":false,"given":"Sun-Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4648-0324","authenticated-orcid":false,"given":"Chan-Duck","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-3455","authenticated-orcid":false,"given":"Yong-Lim","family":"Kim","sequence":"additional","affiliation":[{"name":"Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1614-7635","authenticated-orcid":false,"given":"Seungwoo","family":"Han","sequence":"additional","affiliation":[{"name":"Laboratory for Arthritis and Cartilage Biology, Research Institute of Aging and Metabolism, Kyungpook National University, Daegu 41404, Republic of Korea"},{"name":"Division of Rheumatology, Department of Internal Medicine, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu 41944, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5517-9886","authenticated-orcid":false,"given":"Jeong-Hoon","family":"Lim","sequence":"additional","affiliation":[{"name":"Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea"},{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, 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