{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:48:41Z","timestamp":1762609721599,"version":"build-2065373602"},"reference-count":80,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Centre \u201cKurchatov Institute\u201d","award":["19-14-00268"],"award-info":[{"award-number":["19-14-00268"]}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation (RSF)","doi-asserted-by":"publisher","award":["19-14-00268"],"award-info":[{"award-number":["19-14-00268"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CIMB"],"abstract":"<jats:p>Stroke remains the second leading cause of death worldwide. The development of new therapeutic agents focused on restoring vascular function and neuroprotection of viable tissues is required. In this study the neuroprotective activity of melanocortin-like ACTH(4\u20137)PGP and ACTH(6\u20139)PGP peptides was investigated in rat brain at 24 h after transient middle cerebral artery occlusion (tMCAO). The severity of ischemic damage, changes in the proliferative activity of neuroglial cells and vascularization of rat brain tissue were analyzed. The administration of peptides resulted in a significant increase in the volume density of neurons in the perifocal zone of infarction compared to rats subjected to ischemia and receiving saline. Immunohistochemical analysis of the proliferative activity of neuroglia cells using PCNA antibodies showed a significant increase in the number of proliferating cells in the penumbra and in the intact cerebral cortex of rats receiving peptide treatment. The effect of peptides on vascularization was examined using CD31 antibodies under tMCAO conditions, revealing a significant increase in the volume density of vessels and their sizes in the penumbra after administration of ACTH(4\u20137)PGP and ACTH(6\u20139)PGP. These findings confirm the neuroprotective effect of peptides due to the activation of neuroglia proliferation and the enhancement of collateral blood flow.<\/jats:p>","DOI":"10.3390\/cimb46030133","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T11:26:59Z","timestamp":1709638019000},"page":"2071-2092","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Melanocortin Derivatives Induced Vascularization and Neuroglial Proliferation in the Rat Brain under Conditions of Cerebral Ischemia"],"prefix":"10.3390","volume":"46","author":[{"given":"Vasily V.","family":"Stavchansky","sequence":"first","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadim V.","family":"Yuzhakov","sequence":"additional","affiliation":[{"name":"A. Tsyb Medical Radiological Research Center\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larisa E.","family":"Sevan\u2019kaeva","sequence":"additional","affiliation":[{"name":"A. Tsyb Medical Radiological Research Center\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia K.","family":"Fomina","sequence":"additional","affiliation":[{"name":"A. Tsyb Medical Radiological Research Center\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia E.","family":"Koretskaya","sequence":"additional","affiliation":[{"name":"A. Tsyb Medical Radiological Research Center\u2014Branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva Str. 4B, Obninsk 249036, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina E.","family":"Denisova","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3284-1249","authenticated-orcid":false,"given":"Ivan V.","family":"Mozgovoy","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonid V.","family":"Gubsky","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6964-3405","authenticated-orcid":false,"given":"Ivan B.","family":"Filippenkov","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolay F.","family":"Myasoedov","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana A.","family":"Limborska","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyudmila V.","family":"Dergunova","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1161\/01.STR.0000064841.47697.22","article-title":"Guidelines for the early management of patients with ischemic stroke: A scientific statement from the Stroke Council of the American Stroke Association","volume":"34","author":"Adams","year":"2003","journal-title":"Stroke"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/cphy.c160006","article-title":"Ischemia\/Reperfusion","volume":"7","author":"Kalogeris","year":"2016","journal-title":"Compr. 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